Prospects for the use of ultrasonic disintegrate C. xerosis for the growth of microbial cells of bacterial


  • Helen Isaenko Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine , Ukraine
  • Evgen Babych Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine , Ukraine
  • Volodymir Bilozersky Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine , Ukraine



Introduction. Modern comprehensive use of ultrasound remains relevant. Promising its use is known in medicine, pharmacy, industrial production for the manufacture of therapeutic and prophylactic, immunocorrective and other pharmacological agents, as well as in research and development, experimental studies to obtain disintegrates and derivatives of microorganisms. In the available literature, the use of ultrasonic disintegrates of corynebacteria for the growth of bacterial microbial cells, we have not met. The aim of this work was to study the effect of disintegrates of corynebacterial on individual members of the human normobiocenosis to determine the criteria for predicting the development of normal microflora, which helps prevent diseases caused by the violation of biocenoses. Materials and methods. Ultrasonic disintegrate was obtained by irradiating a suspension of Corynebacterium xerosis with a concentration of 10,0 McFarland units using a generator G3–109 (frequency 40.0 kHz, excitation amplitude U = 15 V, load 5 W, power 0,25 W). It was then concentrated (at 56 ± 1 ° C for 1 hour), kept at 80 ± 1 ° C for 1 hour and used to grow Lactobacillus rhamnosus or Enterococcus faecium. For this purpose, bacterial suspensions with an optical density of 1,0 units on the McF scale were added to the ultrasonic disintegrate of C. xerosis (experiment), nutrient broth with 1% glucose solution (positive control) and 0,9 % sodium chloride solution (negative control), cultured at a temperature of 37 ± 1 ° C. The study of viable cells of microorganisms before and after the growth of microbial mass of representatives (L. rhamnosus or E. faecium) in ultrasonic disintegrates of corynebacteria was performed quantitatively by determining the number of colony-forming units (CFU) per unit volume of test material (lg CFU / ml).
Results & discussion. As a result of growing microbial cells of L. rhamnosus in ultrasonic disintegrates of C. xerosis there was an increase in the bacterial mass of selected bacteria from lg 7,3 ± 0,4 CFU / ml to lg 8,6 ± 0,3 CFU / ml (p = 0,01). When culturing E. faecium in disintegrates of C. xerosis, there was also an increase in enterococcal biomass from lg ͠7,0 CFU / ml to lg 9,5 CFU / ml (p < 0,05). When comparing the studied media, in particular nutrient broth with 1% glucose solution and ultrasonic disintegrate of C. xerosis, the increase in biomass of enterococcal microbial cells, a probable difference between them was not found (p = 0,05), indicating their equal suitability for growing investigated strain of Enterococcus. Instead, more favorable conditions for culturing Lactobacillus in nutrient broth with 1% glucose solution than in disintegrate of C. xerosis (p = 0,03) were found.
Conclusion. The experimental study proved the successful interaction of derivatives of some bacteria with other microorganisms, as well as the importance and prospects of the presented data on the possibility of further determining the criteria for predicting the development of certain representatives of the normobiocenosis and predicting human pathology, including prevention of diseases caused by biocenoses.
Keywords: derivatives of bacteria, biocenosis, normal microflora, disease warning, disintegrates.


Lukavenko IM, Andrushchenko VV, Yazykov OV. The influence of ultrasonic radiation on biological tissues: physical foundations and technological principles. Journal of nano- and electronic physics. 2019. 11. 3. Р. 03008-1–03008-4. URL:

Durnikin DA, Silantyeva MM, Ereshchenko OV. Ultrasound-enhanced cell production of lactic and propionic acid bacteria under submerged cultivation for industrial purposes // Biological Bulletin of Bogdan Chmelnitskiy Melitopol State Pedagogical University. 2016. 6. 2. Р. 287-293.

Akopyan VB, Ershov Yu.A. Fundamentals of the interaction of ultrasound with biological objects (2nd ed.). M. URAIT. 2016. 223 p.

Elapov AA, Kuznetsov NN, Marakhova AI. The use of ultrasound in the extraction of biologically active compounds from plant raw materials, used or promising for use in medicine // Drug development & registration. 2021. 10. 4. Р. 96–116. URL:

Dzah CS. [et al.] The effects of ultrasound assisted extraction on yield, antioxidant, anticancer and antimicrobial activity of polyphenol extracts. Food Bioscience. 2020. 35. Р.100547. URL:

Lavilla I, Bendicho C. Fundamentals of ultrasound-assisted extraction. Extraction of bioactive compounds. 2017. P. 291-316. URL:

Bomko TV, Martynov AV, Nosalskaya TM. Perspective of tinalized microorganisms in the development of safe probiotics. Annals of Mechnikov Institute. 2021. 1. Р. 6-14. URL:

Gao S. et al. Inactivation of bacteria and yeast using high-frequency ultrasound treatment. Water Research. 2014. 60. Р. 93–104. URL:https://doi. 10.1016/j.watres.2014.04.038

Wordon BA, Mortimer B, McMaster LD. Comparative real-time analysis of Saccharomyces cerevisiae cell viability, injury and death induced by ultrasound (20 kHz) and heat for the application of hurdle technology.Food Research International. 2011. 47. 2. Р. 134–139. URL: https://doi. 10.1016/j.foodres.2011.04.038

Osama DM. [et al.] Antimicrobial, antibiofilm and immunomodulatory activity of Lactobacillus rhamnosus and Lactobacillus gasseri against some bacterial pathogens // International Journal of Biotechnology for Wellness Industries. 2017. 6. 1. Р. 12–21. URL:

The prokaryotes: a handbook on the biology of bacteria: in 7 v. / M. Dworkin (editor-in-chief) [et al.]. – 3rd ed. – USA: Springer. 2006. Vol. 4 Bacteria: Firmicutes, Cyanobacteria. 1140 p.

Protocol of the pharmacist (pharmacist) when dispensing over-the-counter drugs. Symptomatic treatment of diarrhea: Order of the Ministry of Health of Ukraine № 875 of October 11, 2013.

Canganella F. [et al.] A microbiological investigation on probiotic pharmaceutical products used for human health // Microbiological Research. 1997. Vol. 152. 2. P. 171–179.

Patent. N. 123122. Sposib oderzhannia metabolitiv probiotychnykh shtamiv bakterii: Isaienko OYu, Knysh OV, Babych YeM, Kivva FV, Horbach TV, Balak OK. 2018. URL:

Isayenko O, Knysh O, Kotsar O, Ryzhkova T, Dyukareva G. Evaluation of anti-microbial activity of filtrates of Lactobacillus rhamnosus and Saccharomyces boulardii against antibiotic-resistant gram-negative bacteria // Regulatory Mechanisms in Biosystems. 2019. 10. 2. Р. 246–251. doi: 10.15421/021937

Atlas RM. Handbook of Microbiological Media. 4th edition. Boca Raton. CRC Press. 2010. 217 p.

Isaienko OYu, Kotsar OV, Ryzhkova TM, Diukareva HI. Producing metabolic complexes of probiotic microorganisms with significant antimicrobial properties. Zaporozhye medical journal. 2021. 23. 1 (124). С. 120–125. URL:

Knysh OV, Isaenko OY, Babych EM,et al. Antimicrobial Activity of Bifidobacteria Derivatives After Storage in a Frozen State. Problems of Cryobiology and Cryomedicine. 2018. 28. 3. Р. 237–248. URL:

Bukharin OV, Semenov AV, Tcherkasov SV. Antagonistic activity of probiotic bacteria. Сlinical microbiology and antimicrobial chemotherapy. 2010. 4. Р. 347–352. URL: ISSN: 1684-4386eISSN: 2686-9586

Badaoui Najjar M, Kashtanov D, Chikindas ML. Natural antimicrobials ε-poly-l-lysine and nisin a for control of oral microflora. Probiotics and antimicrobial proteins. 2009. 1. 2. Р. 143. doi:10.1007/s12602-009-9020-0

Balabekyan TR. et al. Antimicrobial activity of preparations after combined cultivation of lactic acid bacteria and yeast strains.J Anim Physiol and Anim Nutr. 2018. 102. 4. Р. 933–938. URL:

Abdollahi S. et al. Listeria monocytogenes and Salmonella enterica affect the expression of nisin gene and its production by Lactococcus lactis. Microb Pathog. 2018. 123. Р. 28–35. doi: 10.1016/j.micpath.2018.06.024

Moens F. et al. Lactobacillus rhamnosus GG and Saccharomyces cerevisiae boulardii exert synergistic antipathogenic activity in vitro against enterotoxigenic Escherichia coli. Beneficial Microbes. 2019. 10. 8. P. 923–935. URL:



How to Cite

Isaenko, H., Babych, E., & Bilozersky, V. (2022). Prospects for the use of ultrasonic disintegrate C. xerosis for the growth of microbial cells of bacterial . Annals of Mechnikov’s Institute, (3), 62–67.