Synthesizing t-2 toxin conjugates with proteins for the use in immunosensitive test systems

Authors

  • Оксана Станіславівна Гойстер Institute of biochemistry of O. V. Palladin Str. Leontovicha, 9, Kiev, Ukraine, 01601, Ukraine
  • Володимир Євдокимович Кривенчук AP Science Poison Center. L. I. Medvedya MoH Ukraine 6 Heroes of Defense str., Kiev, Ukraine, 03680, Ukraine
  • Григорій Олександрович Хмельницький National University of Life and Environmental NAAS of Ukraine 15 Heroes of Defense str., Kiev, Ukraine, 04034, Ukraine

DOI:

https://doi.org/10.15587/1729-4061.2015.51055

Keywords:

T-2 toxin conjugates, antibody, bovine serum albumin, ovalbumin, hemocyanin, gelatin, thin-layer chromatography

Abstract

We have devised the methods for synthesizing conjugates of mycotoxin T-2 with the proteins of bovine serum albumin, ovalbumin, hemocyanin and gelatin for producing antigens, subsequent immunizing animals, and obtaining specific antibodies. We have proved that T-2 toxin conjugates with bovine serum albumin, ovalbumin and hemocyanin are insoluble in water and are suitable only for the immunization of animals, but cannot be used in immunochemical reactions. Only a T-2 toxin-gelatin conjugate is soluble in water and can be used in immunochemical reactions. Availability of a T-2 toxin-bovine serum albumin conjugate is proved by the method of a thin-layer chromatography.

We have obtained antibodies to T-2 toxin after immunization of rabbits with a T-2 toxin-bovine serum albumin conjugate. A T-2 toxin-gelatin conjugate is used for immobilization on a solid surface of an immunosensor with the effect of a surface plasmon resonance. Antisera with an antibody titer of 1:5000 were selected to calculate the concentration of T-2 toxin by the specified method on the basis of competition. The proven methods for obtaining conjugates, producing immune antisera and identifying antibodies can be the basis for domestic biosensor immunodiagnostic test systems.

Author Biographies

Оксана Станіславівна Гойстер, Institute of biochemistry of O. V. Palladin Str. Leontovicha, 9, Kiev, Ukraine, 01601

Candidate of Biological Sciences, the leading engineer

Department of molecular biology

Володимир Євдокимович Кривенчук, AP Science Poison Center. L. I. Medvedya MoH Ukraine 6 Heroes of Defense str., Kiev, Ukraine, 03680

Doctor of Pharmaceutical Sciences, Senior Research Fellow,

Department of Hygiene polymers and toxic waste.

Григорій Олександрович Хмельницький, National University of Life and Environmental NAAS of Ukraine 15 Heroes of Defense str., Kiev, Ukraine, 04034

Doctor of Veterinary Science, Professor, Academician of NAAS of Ukraine,

Department of Pharmacology and Toxicology

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Published

2015-10-16

How to Cite

Гойстер, О. С., Кривенчук, В. Є., & Хмельницький, Г. О. (2015). Synthesizing t-2 toxin conjugates with proteins for the use in immunosensitive test systems. Eastern-European Journal of Enterprise Technologies, 5(6(77), 4–9. https://doi.org/10.15587/1729-4061.2015.51055

Issue

Vol. 5 No. 6(77) (2015): Technology organic and inorganic substances

Section

Technology organic and inorganic substances

License

Copyright (c) 2015 Оксана Станіславівна Гойстер, Володимир Євдокимович Кривенчук, Григорій Олександрович Хмельницький

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