Influence of preparation of BNO 1030 on pro-/antioxidant status of the blood in experimental type 1 diabetes mellitus

Abstract

Background. Among the mechanisms that lead to the development and deterioration of the diabetes mellitus type 1 (DM1) and its complications, an important role belongs to the intensification of oxidative stress. In the development of many late complications of DM1, in particular, micro- and macroangiopathies, nephropathy and others, oxidative stress is one of the main pathogenetic mechanisms. Objective. To evaluate the effect of phytopreparation BNO 1030 on pro/antioxidant status of the blood in experimental type 1 diabetes mellitus. Materials and methods. The studies were performed on intact male Wistar rats, in which experimental DM1 was modeled. The production of reactive oxygen species in the blood was measured using 2’,7’-dichlorofluorescein diacetate. The content of living, apoptotic and necrotic leukocytes was determined using annexin V conjugated with GFP (green fluorescent protein) and PI (propidium iodide). Results. Against the background of a decrease in body weight and an increase in the concentration of glucose in the blood of diabetic animals, an enhanced formation of reactive oxygen species was established in comparison with the control group of animals that indicates an intensification of the oxidative stress. Under these conditions, the activity of superoxide dismutase decreased, which indicates a violation of regulation between pro- and antioxidant processes. Conclusions. Administration of BNO 1030 to animals with experimental DM1 resulted in the slight intensification of the production of reactive oxygen species both in the control and in the diabetic group, but its effect increased the viability of immune cells, preventing their death. The use of BNO 1030 resulted in an increase of the activity of superoxide dismutase by 10 % compared to the group of diabetic animals.