Experimental evaluation of the influence of gold nanoparticles and cerium dioxide on normal and malignant cells and tissues.

Authors

  • A. G. Reznikov
  • O. A. Falyush
  • N. D. Nosenko
  • O. V. Sachynska
  • L. I. Polyakova
  • A. A. Limareva
  • I. G. Perchyk

DOI:

https://doi.org/10.26641/2307-0404.2017.3.111856

Keywords:

gold nanoparticles, cerium dioxide nanoparticles, reproductive system, aging, prostate cancer

Abstract

The results of experimental studies of the effect of gold nanoparticles (AuNPs) on the organs of the reproductive system of male rats, cells and tissues of androgen-dependent prostate cancer (PCa) are presented, as well as the effect of cerium dioxide nanoparticles (CeO2NPs) on the testes and fertility of aging male rats. Addition of polydisperse colloidal solution of AuNPs (10-50 nm) to the culture medium in a final concentration of 10 μg/ml inhibited the growth of the LNCaP culture, PCa cells, while monodisperse solution of AuNPs (20 nm) caused no effect. The polydisperse colloidal solution of AuNPs arrested the growth of PCa xenografts in mice, when administered parenterally at a dose range of 0.64-6.4 μg/kg body weight. The selectivity of the drug effect on the malignant epithelium is confirmed by signs of its destruction and decrease in the epithelial stromal ratio on histological preparations of xenografts. There was no significant damaging effect of poly- and monodisperse AuNPs solutions on the organs of the reproductive system of male rats when administered for up to two weeks. The stimulating effect of a low dose of CeO2NPs (1 mg/kg b.w.) administered orally on hormonal function of testes and spermatogenesis, proliferative and secretory processes in the prostate of aging male rats was established. However, fertility of animals was reduced in comparison with the control group due to immaturity of the part of spermatozoa. Thus, metal nanoparticles and their salts can have both a stimulating and destructive effect on organs and tissues, which should be taken into consideration when studying their therapeutic potential and in toxicology.

Author Biographies

A. G. Reznikov

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

O. A. Falyush

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

N. D. Nosenko

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

O. V. Sachynska

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

L. I. Polyakova

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

A. A. Limareva

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

I. G. Perchyk

V.P. Komisarenko Institute of Endocrinology and Metabolism,
National Academy of Medical Sciences of Ukraine
Vyshgorodska str., 69, Kyiv, 04114, Ukraine

References

1. Zholobak NM, Olevinskya ZM, Spivak Nya. [Antiviral effect of cerium dioxide nanoparticles stabilized by low-molecular polyacrylic acid]. Microbio­logitcheskiy Zhurnal. 2010;72(3):42-7.). Russian.

2. Dementieva OV, Evdokimov YuM, Zahidov ST. [The influence of gold nanoparticles on the spermato­genesis of mice]. Izvestiya RAN. Ser Biol. 2012;3:279-87. Russian.

3. Salivonyk OA, Sachinskaya OV, Polyakova LI. [ Effect of gold nanoparticles on reproductive organs of male rats]. Klinichna ta Eksperimentalna Patologiya. 2015;14(2):176-9. Ukrainian.

4. Reznikov AG, Salivonyk OA, Sachinskaya OV. [Investigation of the effects of various doses of gold nanoparticles on prostate cancer xenografts].Urologiya. 2014;18(4):17-22. Ukrainian.

5. Falyush OA, Polyakova LI, Sachynska ОV [The changes of epithelial-stromal ratio in transplants of human prostate cancer under application of gold nanoparticles]. Farmakologiya i Likarska Toksykologiya. 2016;50(4-5):86-89. Ukrainian.

6. Maslyakova GN, Pakhomiy SS, Buchar AB. [Morphological changes in the organs of laboratory ani­mals with prolonged oral administration of gold nano­particles]. Saratovskiy Nauchnyi Meditsinskiy Zhurnal. 2013;9(2):208-13. Russian.

7. Nosenko ND, Zholobak NM, Polyakova LI. [Morphofunctional state of the reproductive system of rats in the aging period under the conditions of use of nanoscale]. Fiziologichnyi Zhurnal. 2014;60(1):11-17. Ukrainian.

8. Shcherbakov AB, Ivanov VK, Zholobak NM. [Na­nocrystaline ceria based materials – perspectives for biomedical application]. Biofizika. 2011;56(6):995- 1015. Russian.

9. Spivak NYa, Nosenko ND, Zholobak NM. [The nanocrystalline cerium dioxide raises the functional activity on genesial system of ageing males of rats]. Nanosistemy: Fizika, khimiya, matematika. 2013;4(1):72-77. Russian.

10. Paton BE, Moskalenko VF, Checkman IS. [Na­noscience and Nanotechnology: Technical, Medical and Social Aspects]. Visnyk Natsionalnoi Akademii Nauk Ukrainy. 2009;6:18-29. Ukrainian.

11. Dibkova CM, Reznichenko LS, Gruzina TG. [Evaluation of in vivo DNA-damaging action of gold nanoparticles of different sizes]. Biotekhnologiya. 2010;3(3):66-71. Ukrainian.

12. Usatenko OV, Shcherbakov OB, Kushchevskaya NF, Push GV. [Patent87744 U UA, МПК А61К 31/79, А61К 33/00. A composition containing gold nano­par­ticles, and a method for its preparation. Applicant and Patent Owner Usatenko OV. N 2007200612]. Stated. November 14, 2007; Has published Aug 10, 2009, Bul. N 15. Ukrainian.

13. Petrovskaya YeG. [Application of nano­techno­logy in medicine and pharmacology]. Etyka nano­tekhnologiy ta nanobezpeka. Materialy mizhnarodnogo seminaru. 2014;3;32-34. Russian.

14. Pocheptsov AYa, Magnificent YuI, Filatov BN. [Effect of gold nanoparticles on the proliferative activity of rat germ cells]. Vestnik Volzhskogo GMU. 2012;42(2):47-50. Russian.

15. Filatov BN, Bocharova LI, Klauschek VV. [Production and application of nanomaterials (toxi­cological and hygienic problems)]. Available from: www.MEDLINE.RU. 2015;16:259-66. Russian.

16. Reznichenko LS. [Biochemical effects of the influence of gold nanoparticles on prokaryotic and eukaryotic cells]. PhD Dissert. Thes. 2010;148. Ukrainian.

17. Stavrovska AA. [Cellular Mechanisms of Mul­tiple Drug Resistance of Tumor Cells. Stavrovsk]. Biokhi­miya. 2000;65(1):112-26. Russian.

18. Falyush OA, Sachynska ОV, Polyakova LI. [Des­cription of the reproductive organs of male rats after application of a monodispersed solution of gold nano­particles]. Endokrinologia. 2016;21(3):213-9. Ukrainian.

19. Trahtenberg ІМ, Ulberg ZR, Chekman IS. [Sub­stantiation of expediency of creation and introduction of normative-methodical basis of safety evaluation of medi­cal nanoparticles in Ukraine]. Naukovyi Zhurnal Mini­sterstva Okhorony Zdorovya Ukrainy. 2014;2:20-26. Ukrainian

20. Chekman IS. [Nanopharmacology]. Kyiv, Zad­ruha. 2011;424. Ukrainian.

21. Saleh HM, Soliman OA, Elshazlyn MO. Acute hematologic, hepatologic, and nephrologic changes after intraperitoneal injections of 18 nm gold nanoparticles in hamsters. Int J Nanomed. 2016;11:2505-13.

22. Villiers C, Freitas H, Couderc R. Analysis of the toxicity of gold nanoparticles on the immune system: effect on dendritic cell functions. Nanopart Res. 2010;12(1):55-60.

23. Arnida, Malugin A, Ghandehari. Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres. J Appl. Toxicol. 2010;30(3):212-7.

24. Reznikov AG, Salivonyk OA, Sotkis AG. Asses­sment of gold nanoparticle effect on prostate cancer LNCaP cells. Exp Oncol. 2015;37(2):100-4.

25. Dykman LA, Khlebtsov NG. Gold Nanoparticles in Biology and Medicine: Recent Advances and Prospects. Acta Naturae. 2011;3(2):34-55.

26. Zhou W, Gao X, Liu D. Gold Nanoparticles for In Vitro Diagnostics. Chem Rev. 2015;115(16):10575-636.

27. Roiter Y, Ornatska M, Rammohan AR. Interac­tion of lipid membrane with nanostructured surfaces. Langmuir. 2009;25(11):6287-99.

28. Vandooren J, Berghmans N, Dillen C. Intra­de­r­mal air pouch leukocytosis as an in vivo test for nano­particles. Int J Nanomed. 2013;8(1):4745-56.

29. Li Y, Chen X, Gu N. Computational investigation of interaction between nanoparticles and membranes: hyd­rophobic/hydrophilic effect. J Phys Chem B. 2008;112(51):16647-53.

30. Boulaiz H, Alvarez PJ, Ramirez A. Nano­me­dicine: Application Areas and Development Prospects. Int. J. Mol. Sci. 2011;12(5):3303-21.

31. Giasuddin AM, Jhuma KA, Mujibul AM. Use of Gold Nanoparticles in Diagnostics, Surgery and Me­dicine: A Review. Bangladesh J Med Biochem. 2012;5(2):56-60.

32. Zakhidov ST. Effect of gold nanoparticles on mou­se spermatogenesis. Biologichnyiy Bull. 2012;39:229-36.

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How to Cite

1.
Reznikov AG, Falyush OA, Nosenko ND, Sachynska OV, Polyakova LI, Limareva AA, Perchyk IG. Experimental evaluation of the influence of gold nanoparticles and cerium dioxide on normal and malignant cells and tissues. Med. perspekt. [Internet]. 2017Oct.12 [cited 2024Apr.17];22(3):4-11. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/111856

Issue

Vol. 22 No. 3 (2017)

Section

THEORETICAL MEDICINE

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