DOI: https://doi.org/10.26641/2307-0404.2013.2.15949

Genotype of cytochrome-450 2C9 in patients with tuberculosis

V. V. Filyuk, V. I. Kresyun, P. B. Antonenko, K. K. Rogach, Yu. M. Danylenko, G. V. Mozolevych

Abstract


The aim of present work was to investigate CYP2С9 polymorphism in the south of Ukraine in TB-patients and comparing with the same data of control group by the example of Odessa region. Gene CYP2С9 polymorphism was studied with the help of PCR (polymerase chain reaction) and endonuclease analysis. The blood samples were obtained from patients with new cases of pulmonary TB from Odessa regional antituberculous dispensary and healthy donors in Odessa district station of blood transfusion in the year 2010-12. According to genotype of CYP2C9 of 55 TB-patients, 67,3% persons were the carriers of  homozygote wild gene CYP2C9*1/*1, 21,8 and 3,6% - heterozygote genes CYP2C9*1/*2 and CYP2C9*1/*3. Only 7,2% individuals belonged to carriers of slow metabolizers genotype - CYP2C9*2/*2, *2/*3, *3/*3. In general, the mutated alleles CYP2C9*2 and CYP2C9*3 in TB-males were observed by 1,8 times more frequently than among healthy donors of the same gender. TB-patients older than 30 years more frequently had mutated allele CYP2C9*2, almost by 3 times than individuals from control group.

Keywords


CYP2C9 gene; polymorphism; genotype; tubercuosis

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