Experimental study of modifying influence of nanoaquachelate gold citrate on embryotoxicity of lead acetate in rats.
DOI:
https://doi.org/10.26641/2307-0404.2014.2.27780Keywords:
nanometals, gold citrate, lead acetate, embryogenesisAbstract
Among modern investigations in nanobiotechnology, only a small part of them is devoted to the influence of nanometals on reproductive organs and embryogenesis. Thus, if functions of the separate trace metals are studied well enough, the physiological role of such ultramicroelements as gold is still poorly studied especially in the field of its interaction with other metals. The aim of the research was an experimental study of peculiarities of gold aquananocitrate and lead acetate interaction and their impact on rats’ embryogenesis under condition of combined administration. There were used solutions of lead acetate and citrate gold in experimental models obtained by aquananotehnology. Solutions of metals and nanometals were injected perorally once a day during 19 days of pregnancy: solution of lead acetate in the dose of 0.05 mg/kg and gold aquananocitrate solution in the dose of 1.5 mg/kg. 3 group - control group. Influence of tested substances was assessed by integral and specific indicators using physiological, morphological and quantitative analysis methods. It was established that lead acetate in the dose of 0.05 mg/kg is characterized by severe embryotoxic effect manifested by total embryonic mortality, and reducing number of live fetuses in the offspring. Combined introduction of gold aquananocitrate and lead acetate weakens embryotoxic effect of lead, manifested by increase of corpora lutea number by 30.4%, increase of offspring number by 53.3% and decrease of fetal mortality by 2.3 times.
References
Біопротекторна дія цинку в макро- і нано¬аква¬хелатній формі на ембріогенез щурів за умови свин¬цевої інтоксикації / Е.М. Білецька, І.С. Чекман, Н.М. Онул [та ін.] // Медичні перспективи. – 2013. – №2. – С. 114-119.
Дослідження впливу нанометалів на стан реп¬ро¬дуктивної функції в ембріогенезі / В.Ф. Шаторна, В.І. Гарець, О.О. Савенкова, І.І. Колосова // Таврич. мед.- биол. вестник. – 2013. – Т. 16, № 1 (61), ч.1 – С. 246-251.
Наноматеріали в біології. Основи нановетери¬нарії / за ред. проф. В.Б. Борисевича, проф. В.Г. Кап¬лу¬нен¬ко. – К.: ВД «Авіцена», 2010. – 416 с.
Нанонаука і нанотехнології: технічний, медич¬ний та соціальний аспекти / Б.П. Патон, В.Ф. Моска¬ленко, І.С. Чекман, Б.О. Мовчан // Вісник НАН Ук¬раїни. – 2009. – № 6. – С. 18–26.
Нанотехнології мікронутрієнтів: проблеми, перспективи та шляхи ліквідації дефіциту макро- та мікроелементів / А.М Сердюк, М.П. Гуліч, В.Г. Ка¬п¬лу¬ненко, М.В. Косінов // Журнал АМН України. – 2010. – Т. 16, № 3. – С. 467-471.
Новинюк Л.В. Цитраты – безопасные нутриен¬ты / Л.В. Новинюк // Пищевые ингредиенты. Сырье и добавки. – 2009. – №3 – С. 70-71.
Онул Н.М. Експериментальна оцінка ембріо¬ток¬сичності свинцю як фактору малої інтенсивності / Н.М.Онул // Таврич. мед.-биол. вестник. – 2013. - Т. 16, № 1 (61), ч.1. – С. 165-168.
Скальный А.В. Биоэлементы и показатели эмбриональной смертности лабораторних крыс / А.В. Скальный, С.В. Залавина, С.В. Ефимов // Вест¬ник ОГУ. – 2006. - №2. – С. 78-81.
Способы введения наночастиц золота в биоло¬гическую ткань / В.Е. Родимин, А.В. Гегенава, Н.А. Дер¬жа¬вина, З.М. Гасанова // Вестник оторинола¬ринго¬логии. – 2006. – № 1. – С. 27-31.
Терещенко В.П. Медико-биологические эф¬фек¬ты наночастиц: реалии и прогнозы / В.П. Тере¬щен¬ко, Н.Т. Картель. – К.: Наукова думка, 2010. – 240 с.
Тяжелые металлы внешней среды и их влия¬ние на репродуктивную функцию женщин / А.М. Сер¬дюк, Э.Н. Белицкая, Н.М. Паранько, Г.Г. Шматков. – Днепропетровск: АРТ-ПРЕСС, 2004. – 148 с.
Physicochemical Properties and Cellular Toxicity of Nanocrystal Quantum Dots Depend on their Surface Modification / [A. Hoshno, K. Fujioka, T. Oku, [et al.] // Nano Letters. – 2004. – Vol. 4, N 11. – P. 2163-2169.
Principles for Characterizing the Potential Human Helth Effects From Exposure to Nanomaterials: Elements of a Screening Strategy, Particle, Fibre Toxicology / G. Ober¬dorster, A. Maynard, K. Donaldson [et al.] // Nature Nanotechnology. – 2005. – Vol. 2, N 8. – Р. – 235-246.
Bіlets'ka EM, Chekman ІS, Onul NM. [Biopro¬tective effect of zinc in macro-and nanoaquahelat form in rats embryogenesis under lead intoxication]. Medichnі perspektivi. 2013;2:114-9. Ukrainian.
Shatorna VF, Garets' VІ, Savenkova OO, Kolo¬sova ІІ. [Research of nanometal impact on reproductive function in embryogenesis]. Tavricheskiy mediko-biolo¬gicheskiy vestnik. 2013;16(1)part 1(61):246-51. Ukrai¬nian.
Borisevicha VB, Kaplunenko VG. [Nanomaterials in biology. Bases of nano-veterinary]. K.: VD «Avіt¬sena». 2010;416. Ukrainian.
Paton BP, Moskalenko VF, Chekman ІS, Mov¬chan BO. [Nanoscience and nanotechnologies : technical, medical and social aspects]. Vіsnik natsіonal'noї akademії nauk Ukraїni. 2009;6:18–26. Ukrainian.
Serdyuk AM, Gulіch MP, Kaplunenko VG, Kosіnov MV. [Nanotechnology micronutrients: problems, perspectives and ways to eliminate the deficit of macro-and microelements]. Zhurnal Akademії medichnikh nauk Ukraїni. 2010;16(3):467-71. Ukrainian.
Нovinyuk LV. [Citrates - Safe Nutrients]. Pi¬shche¬vye ingredienty. Syr'e i dobavki. 2009;3:70-71. Russian.
Onul NM. [Experimental estimation of embrio¬toxity of lead as a factor of small intensity]. Tavricheskiy mediko-biologicheskiy vestnik. 2013;16(1)part 1(61):165-8. Ukrainian.
Skal'nyy AV, Zalavina SV, Efimov SV. [Bio¬elements and indicators of embryonic mortality of laboratory rats]. Vestnik OGU. 2006;2:78-81. Russian.
Rodimin VE, Gegenava AV, Derzhavina NA, Gasanova ZM. [Methods for introduction of gold nano¬particles in the biological tissue]. Vestnik otorinola¬ringologii. 2006;1:27-31. Russian.
Tereshchenko VP, Kartel' NT. [Medico-biological effects of nanoparticles: realities and forecasts]. K.: Nau¬kova dumka. 2010;240. Russian.
Serdyuk AM, Belitskaya EN, Paran'ko NM, Shmat¬kov GG. [Heavy metals in the external environment and their impact on the reproductive function of women]. Dnepropetrovsk: ART-PRESS. 2004;148. Russian.
Hoshno A, Fujioka K, Oku T. Physicochemical Properties and Cellular Toxicity of Nanocrystal Quantum Dots Depend on their Surface Modification. Nano Letters. 2004;4(11):2163-9.
Oberdorster G, Maynard A, Donaldson K. Prin¬ciples for Characterizing the Potential Human Helth Ef¬fects From Exposure to Nanomaterials: Elements of a Screening Strategy, Particle, Fibre Toxicology. Nature Nanotechnology. 2005;2(8):235-46.
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