Perspectives of treatment of anemias with cells of fetal liver, immobilized in macroporous alginate-gelatin carriers.
DOI:
https://doi.org/10.26641/2307-0404.2014.2.27781Keywords:
fetal liver cells, macroporous alginate-gelatin scaffold, immune-isolation, transplantation, anemia, erythropoiesisAbstract
Aim of the work was to study possibility of erythropoiesis stimulation by transplantation of fetal liver cells, seeded into macroporous carriers to the rats with post-hemorrhargic anemia, induced by 70% hepatectomy. Fetal liver cells (FLC) were isolated from fetuses of rats with 15 days’ gestation and were cryopreserved. Decryopreserved FLC were seeded into macroporous spongy alginate-gelatin scaffolds, which were covered by alginate capsule and implanted into omentum of rats with modeled liver insufficiency. It was shown that fetal liver cells, immobilized in macroporous scaffolds after implantation have positive effect on red blood count and hemoglobin content, indicating that this approach is promising for the development of new methods of anemia treatment.
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