The correlation between individual biochemical parameters of functional state of the liver in expe¬rimental obstructive jaundice and course administration of L-lysine escinate and glutargine.

Authors

  • V. I. Desjateryk
  • D. V. Mamchur
  • O. V. Kotov
  • S. M. Dronov
  • V. I. Zhyliuk

DOI:

https://doi.org/10.26641/2307-0404.2016.4.89895

Keywords:

experimental obstructive jaundice, superoxide dismutase, malonic dialdehyde, oxidative modification of proteins, bilirubin, hepatic transaminases, correlation

Abstract

The aim of this study was to determine the correlations between markers of oxidant-antioxidant stress in the blood of experimental animals, as well as the content of bilirubin and activity of enzymes in experimental obstructive jaundice. Studies were conducted on 84 adult male Wistar rats (180-230 g). Animals were divided into 4 groups: intact (n = 12), control (n = 24), L-lysine escinate (0,5 mg/kg, n = 24) and glutargin (40 mg/kg, n = 24). The isolation and ligation of the common bile duct below confluence was carried out under general anesthesia. The test preparations were administered 1 time per day, intraperitoneally. Evaluation of the treatment efficacy was carried out on 4th and 10th day of the study. The content of malonic dialdehyde (MDA), aldehyde- (APH) and ketone-phenylhydrazones (CPH), bilirubin and the activity of superoxide dismutase (SOD), catalase, aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were determined using conventional techniques. The correlation links between common markers (bilirubin, AST, ALT) and indicators characterizing oxidant-antioxidant stress (MDA, APH, CPH, SOD, catalase) were determined in the study. High rate of correlation between them was established. The results allow to evaluate indirectly the intensity of oxidative metabolism processes in the liver, which can be used in clinical practice to assess the severity degree of the patients with progressive obstructive jaundice.

Author Biographies

V. I. Desjateryk

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

D. V. Mamchur

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

O. V. Kotov

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

S. M. Dronov

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

V. I. Zhyliuk

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

References

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Published

2016-12-22

How to Cite

1.
Desjateryk VI, Mamchur DV, Kotov OV, Dronov SM, Zhyliuk VI. The correlation between individual biochemical parameters of functional state of the liver in expe¬rimental obstructive jaundice and course administration of L-lysine escinate and glutargine. Med. perspekt. [Internet]. 2016Dec.22 [cited 2024Apr.18];21(4):8-15. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/89895

Issue

Vol. 21 No. 4 (2016)

Section

THEORETICAL MEDICINE

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