The prevalence of cryptosporidiosis in children with diarrhea in Donetsk region, Ukraine

Authors

  • D.V. Yakovenko MHCI “Children’s Territorial Medical Association”, Kramatorsk, Donetsk region, Ukraine,
  • S.I. Pokhil I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine,
  • O.M. Tymchenko I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine,
  • I.I. Torianyk I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine,
  • N.A. Chigirinska I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine,
  • I.A. Kostyria I. Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Kharkiv, Ukraine,

DOI:

https://doi.org/10.22141/2312-413x.7.4.2019.178881

Keywords:

children, cryptosporidiosis, oocyst excretion, specific immunoglobulins

Abstract

An urgent task is to reduce the social and medical burden of protozoal intestinal diseases caused by apicomplexan parasites of the genus Cryptosporidium. This justifies the need to study their prevalence among high-risk populations, which also include children. To determine the prevalence of cryptosporidiosis in Donetsk region (Ukraine), a targeted survey was conducted in 295 children (under the age of 17) with acute intestinal infection, which was clinically manifested by watery diarrhea. According to the results of microscopy of fecal smears (n = 295), concentrated (with formalin-ethyl acetate sedimentation in the fecal concentrator Mini Parasep® SF) and stained (by a modified Ziehl-Neelsen method), the prevalence of cryptosporidium oocyst excretion (OEP) was 4.2 %. All detected oocysts had characteristic morphological and tinctorial properties and were identified by polymerase chain reaction as C.parvum. The presence frequency and titers of specific Ig∑, IgG, IgM and IgA in the blood serum of children (n = 78) were determined in the indirect immune fluorescence reaction with POAgCrypto diagnosticum (purified oocysts of the regional strain C.parvum LNLEID 3/2018, fixed on the surface of the slides). The seroprevalence index of cryptosporidiosis in the cohort of children surveyed reached 21.8 % (with a threshold titer of specific IgG 1 : 16) and exceeded the OEP found in them (p ≤ 0.05). Taking into account the indicators of OEP and seroprevalence index, Donetsk region should be considered as area with relatively low prevalence of cryptosporidiosis in children.

References

Chalmers RM, Davies AP. Minireview: clinical cryptosporidiosis. Exp Parasitol. 2010;124(1):138-146. DOI: 10.1016/j.exppara.2009.02.003.

Wang RJ, Li JQ, Chen YC, Zhang LX, Xiao LH. Widespread occurrence of Cryptosporidium infections in patients with HIV/AIDS: Epidemiology, clinical feature, diagnosis, and therapy. Acta Trop. 2018;187:1617-1625. DOI: 10,1016/j.actatropica.2018.08.018.

Pisarski K. The global burden of disease of zoonotic parasi­tic diseases: Top 5 Contenders for priority consideration. Trop Med Infect Dis. 2019;4(1)44. DOI: 10.3390/tropicalmed4010044.

Bamaiyi PH, Redhuan EM. Prevalence and risk factors for cryptosporidiosis: a global, emerging, neglected zoonosis. Asian Biomed. 2017;10(4):309-325. DOI: 10.5372/1905-7415.1004.493.

Cacciò SM, Chalmers RM. Human cryptosporidiosis in Europe. Clin Microbiol Infect. 2016;22(6):471-480. DOI: 10.1016/j.cmi.2016.04.021.

Putignani L, Menichella D. Global distribution, public health and clinical impact of the protozoan pathogen Cryptosporidium. Interdisciplinary Perspectives Infect Dis. 2010;2010(ID 753512):39. DOI: 10.1155/2010/753512.

Cacciò SM, Putignani L. Epidemiology of human cryptosporidiosis. In Cryptosporidium: Parasite and Disease. Springer-Verlag Wien. 2014:43-79. DOI: 10.1007/978-3-7091-1562-6_2.

Sánchez A, Prats-van der Ham M, Tatay-Dualde J et al. Zoonoses in Veterinary Students: A Systematic Review of the Li­terature. PLOS One. 2017;12(1):e0169534. DOI: 10.1371/journal.pone.0169534.

Khalil IA, Troeger C, Rao PC et al. Morbidity, mortality, and long-term consequences associated with diarrhoea from Cryptosporidium infection in children younger than 5 years: a meta-analyses study. Lancet Global Health. 2018;6(7):e758-e768. DOI: 10.1016/S2214-109X(18)30283-3.

Korpe PS, Valencia C, Haque R et al. Epidemiology and Risk Factors for Cryptosporidiosis in Children From 8 Low-income Sites: Results From the MAL-ED Study. Clin Infect Dis. 2018;67(11):1660-1669. DOI: 10.1093/cid/ciy355.

Kotloff KL, Nataro JP, Blackwelder WC et al. Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study. Lancet. 2013;382(9888):209-222. DOI: 10.1016/S0140-6736(13)60844-2.

Exum NG, Pisanic N, Granger DA et al. Use of Pathogen-Specific Antibody Biomarkers to Estimate Waterborne Infections in Population-Based Settings. Curr Environ Health Rep. 2016;3(3):322-334. DOI: 10.1007/s40572-016-0096-x.

Becker DJ, Oloya J, Ezeamama AE. Household socioeconomic and demographic correlates of Cryptosporidium seropositi­vity in the United States. PLoS Negl Trop Dis. 2015;9(9):e0004080. DOI: 10.1371/journal.pntd.0004080.

Elwin K, Chalmers RM, Hadfield SJ et al. Serological responses to Cryptosporidium in human populations living in areas reporting high and low incidences of symptomatic cryptosporidiosis. Clin Microbiol Infect. 2007;13(12):1179-1185. DOI: 10.1111/j.1469-0691.2007.01823.x.

Casemore D. Towards a US national estimate of the risk of endemic waterborne disease — sero-epidemiologic studies. J Water Health. 2006;4(2):121-163. DOI: 10.2166/wh.2006.021.

Elshahawy I. A serological survey on cryptosporidiosis in Najran region, Southwestern Saudi Arabia with reference to some epidemiological features of the infection. Int J Adv Med. 2017;4(4):887-893. DOI: 10.18203/2349-3933.ijam20172606.

Khan A, Shams S, Khan S, Khan MI, Khan S, Ali A. Evaluation of prevalence and risk factors associated with Cryptosporidium infection in rural population of district Buner, Pakistan. PLoS ONE. 2019;14(1):e0209188. DOI: https://doi.org/10.1371/journal.pone. 0209188.

Pokhil SI, Tymchenko OM, Yakovenko DV, Chigirinskaya NA, Kostyria IA, Nesterenko AM. Practical experlence in using the Mini Parasep faecal parasite concentrator for Cryptosporidium spp. oocysts detection in stool. Annals of Mechnikov Institute. 2018;3:33-40. DOI: 10.5281/zenodo.1456534.

Yakovenko DV, Pokhil SI, Tymchenko OM, Chigirinskaya N.A, Kostyria IA. Evaluation of differential efficiency of staining methods for Cryptosporidium oocysts. Bulletin of Problems Biology and Medicine. 2018;4(146):198-213. DOI: 10.29254/2077-4214-2018-4-1-146-198-203 (in Ukrainian).

Braz LM, Amato VN, Ferrari CI et al. Human cryptosporidiosis: detection of specific antibodies in the serum by an indirect immunofluorescence. Rev Saude Publica. 1996;30(5):395-402. DOI: 10.1590/s0034-89101996000500001.

Lakin GF. Biometrics: guide. 4th ed., revised and updated. Moscow: Higher school, 1990. 352 p. ISBN 5-06-000471-6 (in Russian).

Order of the Ministry of Health of Ukraine No. 905 dated December 28, 2015 «On approval of the criteria for determining the cases of infectious and parasitic diseases that are subject to registration». www.moz.gov.ua/ua/portal/dn_20151228_0905.html (in Ukrainian).

Checkley WA, White AC, Jaganath D et al. A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium. Lancet Infect Dis. 2015;15(1):85-94. DOI: 10.1016/S1473-3099(14)70772-8.

Ghazy AA, Abdel-Shafy S, Shaapan RM. Cryptosporidiosis in animals and man: 2. Diagnosis Asian J Epidemiol. 2015;8(4):84-103. DOI: 10.3923/aje.2015.84.103.

Chalmers RM, Katzer F. Looking for Cryptosporidium: the application of advances in detection and diagnosis. Trends Parasitol. 2013;29(5):237-251. DOI: 10.1016/j.pt.2013.03.001.

Ryan U, Fayer R, Xiao L. Cryptosporidium species in humans and animals: current understanding and research needs. Para­sitol. 2014;141:1667-1685. DOI: 10.1017/S0031182014001085.

Painter JE, Hlavsa MC, Collier SA, Xiao L, Yoder JS. Centers for Disease Control and Prevention. Cryptosporidiosis Surveillance — United States, 2011-2012. MMWR Surveillance Summaries. 2015;64(3):1-14. Available from: https://www.cdc.gov/mmwr/pdf/ss/ss6403.pdf.

Lee J-K, Han E-T, Huh S, Park W-Y, Yu J-R. A Hospital-Based Serological Survey of Cryptosporidiosis in the Republic of Korea. Korean J Parasitol. 2009;47(3):219-225. DOI: 10.3347/kjp.2009.47.3.219.

Farkas K, Plutzer J, Moltchanova E et al. Serological responses to Cryptosporidium antigens in inhabitants of Hungary using conventionally filtered surface water and riverbank filtered drinking water. Epidemiol Infect. 2015;143(13):2743-2743. DOI: 10.1017/S0950268814003859.

Priest JW, Bern C, Xiao L, et al. Longitudinal analysis of cryptosporidium species-specific immunoglobulin G antibody responses in Peruvian children. Clin Vaccine Immunol. 2006;13(1):123-131. DOI: 10.1128/CVI.13.1.123-131.2006.

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Published

2019-08-01

Issue

Vol. 7 No. 4 (2019)

Section

Original Researches

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