Family foci of enterovirus infection (clinical cases)


  • N.V. Matsiyeuskaya MD, PhD, Associate Professor, Head of the Department of Infectious Diseases, Grodno State Medical University, Grodno, Republic of Belarus, e-mail:,
  • A.L. Sovkich Assistant Lecture at the Department of Infectious Diseases, Grodno State Medical University, Grodno, Republic of Belarus, e-mail:,
  • T.V. Amvroseva MD, PhD, Sc.D., Head of Laboratory of Natural Reservoir Infections, The Republican Research and Practical Center for Epidemiology and Microbiology, Minsk, Republic of Belarus, e-mail:,
  • Yu.A. Shilova Junior Research Scientist of Laboratory of Natural Reservoir Infections The Republican Research and Practical Center for Epidemiology & Microbiology, Minsk, Republic of Belarus, e-mail:,



enterovirus infection, non-polio enteroviruses, intrafamilial focus of infection, ECHO-3, ECHO-6, polymerase chain reaction


The article describes two intrafamilial foci of enterovirus infection (EVI), caused by ECHO-3 and ECHO-6 viruses. In the first group, 6 children aged from 3 months to 4 years were found (average age — 18.0 ± 4.3 months), 3 boys (50 %) and 3 girls (50 %) with various clinical manifestations of EVI associated between themselves epidemiologically (siblings of one fami­ly, who used water from one draw-well and had close household contact). ECHO-3 virus was detected in the clinical material of the examined children. The second outbreak involved two children from the same family, who simultaneously suffered from aseptic meningitis in 2017. Cerebrospinal fluid of both children was examined by reverse transcription polymerase chain reaction (RT-PCR) (Republican Research and Practical Center for Epidemiology and Microbiology, Minsk), RNA EV was detected in both samples. After RNA sequencing, ECHO 6 was detected in both samples. The verification in both cases was performed by various clinical and laboratory methods (general clinical methods, enzyme-linked immunosorbent assay with detection of antibo­dies to EV and antigens of EV in feces, RT-PCR with subsequent EV RNA sequencing and determination of virus serotype). In two cases of the disease, the diagnosis of EVI was made epidemiologically without laboratory detection of RNA EV. PCR with the determination of EV RNA in feces and cerebrospinal fluid showed the greatest effectiveness. In the described outbreaks, RNA of enteroviruses was not detected in the blood in any sample.


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Original Researches