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Roberto Fernandez-Lafuente

Roberto Fernandez-Lafuente
Consejo Superior de Investigaciones Científicas, Spain

Scopus profile: link


Professional (scientific) interests

- biocatalysis
- enzyme immobilization
- enzyme stabilization
- enzyme purification
- bioprocess dedign
- biosensor design


PhD, UAM Sciences
Degree in Biology, UCM


Selected Publications:

1. Greco-Duarte, J., Collaço, A. C. A., Costa, A. M. M., Silva, L. O., Da Silva, J. A. C., Torres, A. G. et. al. (2019). Understanding the degree of estolide enzymatic polymerization and the effects on its lubricant properties. Fuel, 245, 286–293. doi: 

2. De Andrades, D., Graebin, N. G., Kadowaki, M. K., Ayub, M. A. Z., Fernandez-Lafuente, R., Rodrigues, R. C. (2019). Immobilization and stabilization of different β-glucosidases using the glutaraldehyde chemistry: Optimal protocol depends on the enzyme. International Journal of Biological Macromolecules, 129, 672–678. doi: 

3. Tacias-Pascacio, V. G., Torrestiana-Sánchez, B., Dal Magro, L., Virgen-Ortíz, J. J., Suárez-Ruíz, F. J., Rodrigues, R. C., Fernandez-Lafuente, R. (2019). Comparison of acid, basic and enzymatic catalysis on the production of biodiesel after RSM optimization. Renewable Energy, 135, 1–9. doi: 

4. Ávila, S. N. S., Gutarra, M. L. E., Fernandez-Lafuente, R., Cavalcanti, E. D. C., Freire, D. M. G. (2019). Multipurpose fixed-bed bioreactor to simplify lipase production by solid-state fermentation and application in biocatalysis. Biochemical Engineering Journal, 144, 1–7. doi: 

5. Andrades, D. de, Graebin, N. G., Ayub, M. A. Z., Fernandez-Lafuente, R., Rodrigues, R. C. (2019). Physico-chemical properties, kinetic parameters, and glucose inhibition of several beta-glucosidases for industrial applications. Process Biochemistry, 78, 82–90. doi: 

6. Rojas, M., Amaral-Fonseca, M., Zanin, G., Fernandez-Lafuente, R., Giordano, R., Tardioli, P. (2019). Preparation of Crosslinked Enzyme Aggregates of a Thermostable Cyclodextrin Glucosyltransferase from Thermoanaerobacter sp. Critical Effect of the Crosslinking Agent. Catalysts, 9 (2), 120. doi: 

7. Pinto, M. C. C., de Souza e Castro, N. L., Cipolatti, E. P., Fernandez-Lafuente, R., Manoel, E. A., Freire, D. M. G., Pinto, J. C. (2018). Effects of Reaction Operation Policies on Properties of Core-Shell Polymer Supports Used for Preparation of Highly Active Biocatalysts. Macromolecular Reaction Engineering, 13 (1), 1800055. doi: