Изображение профиля

Roberto Fernandez-Lafuente

Roberto Fernandez-Lafuente
Consejo Superior de Investigaciones Científicas, Испания

Scopus Profile: http://www.scopus.com/authid/detail.url?authorId=35552449900

ID ORCID: http://orcid.org/0000-0003-4976-7096

Professional (scientific) interests

- biocatalysis
- enzyme immobilization
- enzyme stabilization
- enzyme purification
- bioprocess dedign
- biosensor design

Qualifications:

PhD, UAM Sciences
Degree in Biology, UCM

 Publications (Selected):

  1. Álvaro, G., Fernández-Lafuente, R., Blanco, R.M. & Guisán, J.M. 1990. "Immobilization-stabilization of penicillin G acylase from Escherichia coli". Appl. Biochem. Biotechnol. 26, 181-195.
  2. Guisán. J.M., Álvaro. G. & Fernández-Lafuente, R. 1990. "Immobilisation-stabilisation of penicillin G acylase. An integrated approach" Ann. N.Y. Acad. Sc. 613, 552-559
  3. Álvaro, G., Fernández-Lafuente, R., Blanco, R.M. & Guisán, J.M. 1991. "Stabilizing effect of penicillin G sulfoxide, a competitive inhibitor of penicillin G acylase: its practical applications" Enzyme Microb. Technol. 13, 1-5.
  4. Fernández-Lafuente, R., Álvaro. G., Blanco, R.M. & Guisán. J.M. 1991. "Equillibrium controlled synthesis of cephalotin in monophasic water-organic cosolvents systems catalysed by stabilised derivatives of penicillin G acylase" Appl. Biochem. Biotechnol. 27, 277-280.
  5. Guisán, J.M., Bastida, A, Cuesta, C., Fernández-Lafuente, R. & Rosell, C.M. 1991. "Immobilisation-stabilisation of chymotrypsin by covalent attachment to aldehyde agarose gels" Biotechnol. Bioeng. 39, 1144-1152.
  6. Fernández-Lafuente, R., Rosell, C.M. & Guisán, J.M. 1991. "Enzyme reaction engineering: design of the synthesis of antibiotics catalysed by stabilised penicillin G acylase in the presence of organic solvents" Enzyme Microb. Technol. 13, 898-905.
  7. Fernández-Lafuente, R., Rosell, C.M., Álvaro, G. & Guisán, J.M. 1992. "Additional stabilisation of penicillin G acylase by controlled chemical modification of immobilised/stabilised  derivatives" Enzyme Microb. Technol. 14, 489-495.
  8. Guisán. J.M., Blanco, R.M., Bastida. A., Fernández-Lafuente, R., Cuesta, C. & Halling, P.J. 1992. "Fully dispersed and covalent attached chymotrypsin derivatives as industrial catalyst in biphasic systems: activity, stability and reactivation" Ann. NY Acad. Sci. 672, 158-166.
  9. Álvaro. G., Fernández-Lafuente. R., Rosell, C.M., Blanco, R.M., García-López, J.L. & Guisán. J.M. 1992. "Penicillin G acylase from Kluyvera citrophila. New choice as industrial enzyme" Biotechnol. Lett. 12, 285-290.
  10. Fernández-Lafuente, R., Rosell. C.M., Rodríguez, V., Santana, C., Soler, G., Bastida, A. & Guisán, J.M. 1993. "Preparation of activated supports containing low pK amino groups. A new tool for protein immobilisation via the carboxyl coupling method" Enzyme Microb. Technol. 15, 546-550.
  11. Rosell, C.M., Fernández-Lafuente, R. & Guisán, J.M. 1993. "Resolution of racemic mixtures by means of synthesis reactions catalysed by immobilised derivatives of the enzyme penicillin G acylase" J. Mol. Catal. 84, 365-371.
  12. Guisán, J.M., Rodríguez, V., Soler, G., Santana, C., Fernández-Lafuente, R., Bastida. A., Rosell, C.M., López, R., Fernández-Mayoralas, A. & Martín-Lomas, M. 1993. "Synthesis of oligosaccharides of pharmaceutical  interest catalysed by immobilised-stabilised derivatives of different ß-galactosidases" J. Mol. Catal. 84, 373-379.
  13. Guisán. J.M., Álvaro, G., Fernández-Lafuente, R., Rosell, C.M., García-López, J.L. & Tagliatti, A. 1993. "Stabilization of an heterodimeric  enzyme by multipoint covalent immobilisation: Penicillin G acylase from Kluyvera citrophila" Biotechnol. Bioeng. 42, 455-464.
  14. Calvete, J.J., Mann, K., Schafer, W., Fernández-Lafuente, R. & Guisán, J.M. 1994 "Proteolytic degradation of the RGD-binding and RGD non-binding conformers of human platelet integring glycoprotein IIb/IIa: clues for identification of regions involved in the receptor's activation" Biochem. J. 298, 1-7.
  15. Bes, T., Fernández-Lafuente, R., Rosell, C.M., Gómez-Moreno, C. & Guisán, J.M. 1994. "Selective oxidations by using oxygen as oxidizing agent: immobilisation and stabilisation of FNR, a NADP+ regenerating enzyme". STUDIES IN SURFACE SCIENCE AND CATALYSIS 82: 685-692