Natural origin immunomodulators influence on cellular immunity indices in rabbits blood under stress
DOI:
https://doi.org/10.15587/2313-8416.2015.37406Keywords:
rabbits, pre-slaughter stress, spleen extracts, T- and B-lymphocytesAbstract
Aim. The article is devoted to the researching of T- and B-lymphocytes relative amount in the rabbits blood after using of plant and animal origin immunomodulators under stress.
Methods. The relative amount of T- and B-lymphocytes and its some populations in the reaction of spontaneous rоsetting with the ram erythrocytes in the blood was determined. Thus, the differentiated count of rоsetting lymphocyte with the different functional activity degree was conducted. The spleen extracts added to the rabbits feed by aerosol introduction method.
Results. T- and B-lymphocytes amount and functional activity T- and B-cell immunity was stimulated after additional supplementary to rabbits diet of experimental group natural origin immunomodulators. Spleen extract polyamines as the immunomodulators and antistressors most effectively influenced on some T- and B-cell immunity indices before slaughter: amount of T-(Тh-0) and B-(3–5)lymphocytes were decreased.
Conclusions. The results which obtained in experiment can to use in researches of cell immunity indices on farm animals for organism resistance increasing and correction their pre-slaughter stress.
References
Lapovecz, L. Ye., Lucyk, B. D., Lebed, G. B. (2014). Manual of Laboratory Immunology, Lviv, 292. [in Ukrainian]
Grabovskyi, S. S. (2014). Natural origin immunomodulators influence on cellular immunity indices and cortisol level in rats blood at pre-slaughter stress. Studia Biologica, 8 (1), 93–102. [in Ukrainian]
Chernushenko, E. F. (2007). Local immunity: diagnostics of its violations and possibility of correction. Art of treatment, 7(43), 61–67. [in Russian]
Groer, M., Wolfe, S. Park, C. R. (2013). Reduction of hair glucocorticoid levels in an animal model of post-traumatic stress disorder (PTSD) Brain, Behavior, and Immunity, 68 (32), 20. doi: 10.1016/j.bbi.2013.07.080
Kuzmenko, O. V. (2011). Daily rhythm of immune system indexes rats and radio sensitivity. Experimental and clinical medicine, 1 (50), 132–141. (in Ukrainian).
Kyseleva, N. M., Kuzmenko, L. H. Nkane Nkoza, M. M. (2012). Stress and lymphocytes, Paediatrics, 91(1), 137–143. [in Russian]
Marvar, P. J., Vinh, A., Thabet, S. et al. (2012). T lymphocytes and vascular inflammation contribute to stress-dependent hypertension. Biological psychiatry, 71 (9), 774–782. doi: 10.1016/j.biopsych.2012.01.017
Salyha, N. (2012). Т- and В-cells immunity at the condition introduction L-glutamic acid. Visnyk of Lviv Univ. Biology Series, 58, 80–84. [in Ukrainian]
Naduge, M., Didac, C-G., Frank, M. (2011). Polyamines in aging and disease. AGING, 3 (8), 1–16.
Reyes-Becerril, M., Ascencio-Valle, F., Tovar-Ramнrez, D. et al. (2010). Effects of polyamines on cellular innate immune response and the expression of immune-relevant genes in gilthead seabream leucocytes. Fish Shellfish Immunol., 30, 248–254. doi: 10.1016/j.fsi.2010.10.011
Nitta, T., Igarashi, K., Yamashita, A. et al. (2001). Involvement of Polyamines in B Cell Receptor-Mediated Apoptosis: Spermine Functions as a Negative Modulator. Experimental Cell Research, 265 (1), 174–183. doi: 10.1006/excr.2001.5177
Grabovskyi, S. S. (2014). Polyamines content and its correction in broiler chickens blood and tissues at pre-slaughter stress. The Animal Biology, 16 (2), 18–25. [in Ukrainian]
Grabovskyi S. S. (2013). Extracting of biologically active substances of spleen with the application an ultrasound. Proceedings SWorld. Ivanovo: Markova A. D., 4 (49), 3–6. [in Russian]
Official Journal of the European Union L276/33. Directive 2010/63/EU of the european parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010.
Vlizlo, V. V., Fedoruk, R. S., Ratych, I. B. et al. (2012). Laboratory methods of investigation in biology, stock-breeding and veterinary : Reference book ; Edited by V. V. Vlizlo. Lviv : SPOLOM, 764. [in Ukrainian]
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