The effect of nitrogen oxide on the formation of chromosomal instability in human lymphocytes under the co-mutagenic modification
DOI:
https://doi.org/10.15587/2313-8416.2015.37451Keywords:
nitrogen oxide, co-mutagen, ascorbic acid, blood lymphocytes, chromosome aberration, proliferative potentialAbstract
Aim. Identify the features of formation of chromosomal instability in lymphocytes of nominally healthy individuals under conditions of combined action of nitrogen oxides (NO) and ascorbic acid (AA), depending on their concentration (study in vitro).
Material and methods. Culture of human peripheral blood lymphocytes (PBL) with metaphase analysis of chromosome aberrations. Nitrosated glutathione as the main transport form of NO injected into PBL culture in the concentration range 0,25-0,5-1,0 mmol / ml of blood. As co-mutagen used AA in the concentration range 20,0-40,0-80,0 mg / ml of blood, corresponding therapeutic drug concentrations, as well as 2 and 4 times exceeding its value.
Results. Discovered co-mutagenic effect of AA manifested in increasing the overall frequency of chromosomal aberrations in 1.7 and 1.4 times respectively the concentration of NO. The spectrum induced damage presents a whole by chromatid type aberrations that occur mainly by single-strand DNA breaks and kept stable in a number of cell generations. In all experimental observation points an additional impact of AA (regardless of concentration) strengthened depressing effect of NO in concentration of 1.0 mmol / ml of blood on proliferative potential of cells.
Conclusions. By the action of chemical mutagens NO on blood lymphocytes of nominally healthy persons an additional impact of AA in high concentration (80 mg / ml of blood) complicates chromosome instability of cells, increasing the level chromatid aberrations in 2 times, and inhibits their proliferative potential
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Copyright (c) 2015 Емілія Анатоліївна Дьоміна, Віктор Михайлович Михайленко, Олена Петрівна Пилипчук
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