The study of microspore morphogenesis activity In Vitro in anther culture of rye

Authors

  • Ірина Сергіївна Замбріборщ Plant Breeding & Genetics Institute National Center of Seed and Cultivar Investigation Ovidiopolskaya road 3, Odessa, 65036, Ukraine, Ukraine
  • Оксана Леонідівна Шестопал Plant Breeding & Genetics Institute National Center of Seed and Cultivar Investigation Ovidiopolskaya road 3, Odessa, 65036, Ukraine, Ukraine
  • Світлана Олександрівна Ігнатова Plant Breeding & Genetics Institute National Center of Seed and Cultivar Investigation Ovidiopolskaya road 3, Odessa, 65036, Ukraine, Ukraine
  • Зоя Олександрівна Мазур Vekhnyacha Research Breeding Station Verkhnyachka Town, Khrystynivka district, Cherkasy Radion, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.38752

Keywords:

rye, androgenesis in vitro, new growth, regeneration

Abstract

The morphogenetic capacity of rye microspores in anther culture was investigated. Testing the influence of 2 inductive nutrient medium (190-2 and W14) for passing the first stage of androgenesis in vitro at anther culture of 4 samples of rye were conducted. It is shown that both nutrient media suitable for formation of new growth in anther culture of rye of Ukrainian selection. The five albino regenerates were obtained

Author Biographies

Ірина Сергіївна Замбріборщ, Plant Breeding & Genetics Institute National Center of Seed and Cultivar Investigation Ovidiopolskaya road 3, Odessa, 65036, Ukraine

PhD

Tissue culture laboratory

Оксана Леонідівна Шестопал, Plant Breeding & Genetics Institute National Center of Seed and Cultivar Investigation Ovidiopolskaya road 3, Odessa, 65036, Ukraine

PhD

Tissue culture laboratory 

Світлана Олександрівна Ігнатова, Plant Breeding & Genetics Institute National Center of Seed and Cultivar Investigation Ovidiopolskaya road 3, Odessa, 65036, Ukraine

Professor

Tissue culture laboratory 

Зоя Олександрівна Мазур, Vekhnyacha Research Breeding Station Verkhnyachka Town, Khrystynivka district, Cherkasy Radion

PhD

Selection and Seed Department

References

Deimling, S., Jain, M. S., Sopory, S. K., Veilleux, R. E. Flehighaus-Roux, T. (1997). Haploidy in rye. Current Plant Science and Biotechnology in Agriculture, 181–204. doi: 10.1007/978-94-017-1862-2_10

Rakoczy-Trojanowska, M., Smiech, M., Malepszy, S. (1997). The influence of genotype and medium of rye (Secale cereale L.) anther culture. Plant Cell Tissue Organ Culture, 48, 15–21.

Immonen, S., Anttila, H. (2000). Media composition and anther plating for production of androgenic green plants from cultivated rye (Secale cereale L.). Plant Physiology, 156 (2), 204–210. doi: 10.1016/s0176-1617(00)80307-7

Bolibok, H., Rakoczy-Trojanowska, M., Bolibok, H. (2006). Genetic mapping of QTLs for tissue culture response in plants. Euphitica, 149 (1-2), 73–83. doi: 10.1007/s10681-005-9055-6

Zheng, M. Y. (2003). Microspore culture in wheat (Triticum aestivum) – doubled haploid production via induced embryogenesis. Plant Cell Tissue Organ Culture, 73, 213–230.

Agache, S., De Buyser, J., Henry, Y., Snape, J. (1988). Studies of the genetic relationship between anther culture response and somatic tissue culture abilities in wheat. Plant Breeding, 100 (1), 26–33. doi: 10.1111/j.1439-0523.1988.tb00213.x

Grobe, D., Deimling, S., Geiger, H. (1996). Mapping of genes for anther culture ability in rye by molecular markers. Vortr. Pflanzenzuchtg, 35, 282–283.

Logue, S.; Jain, M. S., Sopory, S. K., Veilleux, R. E. (Eds.) (1996). Genetic stability in microspore-derived doubled haploids. Current Plant Science and Biotechnology in Agriculture, 1–51. doi: 10.1007/978-94-017-0477-9_1

Ouyang, J., Jia, S., Zhang, C., Chen, X., Feng, G. (1989). A new synthetic medium (W14 medium) for wheat anther culture. Annual Report, Institute of Genetics, Academia Sinica, 91–92.

Wang, X., Hu, H. (1984). The effect of potato II medium for triticale anther culture. Plant Science Letters, 36 (3), 237–239. doi: 10.1016/0304-4211(84)90175-5

Pausheva, Z. P. (1988). Workshop on cytology plants: Textbook [for students of High school]. Moscow: Agropromizdat, 270.

Tenhola-Roininen, T. (2009). Rye doubled haploids. Finland, 93.

Lukyanyuk, S. F., Ignatova, S. A. (1980). Methods for tissue and organ culture in plant breeding. Methodical recommendations. Odessa, 21.

Published

2015-03-22

Issue

Section

Biological sciences