Effect of nitric oxide on cholinoreceptors of heart of animals at damage by adrenaline in terms of sex and activity of the gonads
Keywords:acetylcholine, bradycardia duration, bradycardia intensity, regulation, gender, adrenaline myocardial damage, gonadectomy, substitutive hormonotherapy, nitric oxide
Methods. Experiments are conducted on 216 white mature rats of both sexes (180–240 g), which is modeled adrenaline myocardial damage (AMD) by intramuscular epinephrine administration (1 mg/kg). Activity of synthesis of nitric oxide is changed by administration of L-arginine donator (600 mg/kg) and non-selective inhibitor L-NAME (25 mg/kg) in the abdomen for 15 minutes to AMD reproduction . Gonadectomy is performed surgically. Hormone replacement therapy after gonadectomy is conducted in females by administration of hexestrol (0.1 mg/kg) and progesterone (5 mg / rat), and in males – administration of testosterone (200 mg/100 g). [5, 6]. Research of heart sensitivity to exogenous acetylcholine animals is performed after administration of solution of acetylcholine chloride in the jugular vein. Chronotropic effect was assessed in intensity and duration of occurred bradycardia. Research of heart sensitivity to endogenous acetylcholine is performed by analyzing changes in heart rate at an electrical stimulation of the right vagus nerve . The effect was evaluated by the intensity of bradycardia.
Results. Against the background of the administration of L-arginine, especially in the development of necrotic damage, deficiency of sex hormones increases the sensitivity of myocardial cholinergic receptors in males (IBah increased by 39% in the control, 5.7 and 3.2 times at 1 and 24 hours of experience) and a completely different reaction in females - a decline of 26.4% (in control) 2.1 times to 1 hour and increased 2.1 times in 24 hours. Using L-NAME, change the background of hormones does not affect the sensitivity of cholinergic receptors, while the intensity of the release of acetylcholine in the synaptic cleft (the electrical stimulation n.Vagus) in females in a much greater extent than in males increase at gonadectomy, including development of adrenaline myocardial damage.
Conclusions. Males and females respond differently to change the sensitivity of cholinergic receptors and the intensity of the release of the neurotransmitter acetylcholine in the presynaptic cleft when used donator blocker system and nitric oxide. Gonadectomy showed a direct relationship of influence of nitric oxide system on the heart condition of cholinergic regulation of sex hormones, which showed excellent dynamics change. Substitutive hormonotherapy most approximates the laws of choline reactivity of the heart, especially in the development of necrotic myocardium in the process to natural (under conditions of normal sex hormones)
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