Regulation of lactate dehydrogenase activity by ascorbic acid in organs of rats with hypoxia of closed space

Authors

  • Олександр Костянтинович Будняк Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine
  • Сергій Анатолійович Петров Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine
  • Сніжана Сергіївна Чернадчук Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine
  • Катерина Юріївна Ожерельєва Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine
  • Валерія Володимірівна Хмельницька Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine
  • Ілона Олегівна Кравчук Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine
  • Андрій Вікторович Сорокін Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.45970

Keywords:

hypoxia of closed space, ascorbic acid, lactate dehydrogenase

Abstract

The research of the effects of different doses of ascorbic acid (AA) on activity of lactate dehydrogenase (LDG) in the organs of white nonlinear rats with hypoxia of closed space (HCS) has been conducted. Injections of АА to rats for 30 minutes of HCS reduced the LDG activity in all organs of hypoxic animals

Author Biographies

Олександр Костянтинович Будняк, Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

Candidate of Biological Sciences, Associate Professor

Сергій Анатолійович Петров, Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

Doctor of Biological Sciences, Professor

Сніжана Сергіївна Чернадчук, Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

Candidate of Biological Sciences, Associate Professor

Катерина Юріївна Ожерельєва, Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

Graduate student

Валерія Володимірівна Хмельницька, Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

student

Ілона Олегівна Кравчук, Department of Biochemistry Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

bachelor

Андрій Вікторович Сорокін, Odessa I. I. Mechnikov National University 2 Dvoryanskaya str., Odessa, Ukraine, 65082

Candidate of Biological Sciences, Associate Professor

Department of  Biochemistry

References

Karpov, L. M. (1994). Realizacija specificheskoj aktivnosti funkcional'no svjazannyh vitaminov gruppy B, ih proizvodnyh i kompleksov pri razlichnyh sostojanijah organizma [Implementation of the specific activity of functionally related B vitamins, their derivatives and complexes in different states of the organism]. Odessa, 505.

Sorokіn, A. V. (2002). Vzaemodіja nіkotinovoi kisloti z іnshimi vіtamіnami v realіzacіi funkcіj za rіznih stanіv tvarin [Interaction of Nicotinic Acid with other B vitamins in the realization of the functions of the different states of animals]. Taurida National V. I. Vernadsky University. Simferopol, 19.

Karpov, L. M., Shevchenko, L. A., Poltavceva, N. V., Budnjak, O. K., Sorokіn, A. V., O. M. Yershova, O. M., Afra Mohamed Abdul Rashid, Apolinarija Mukarurangva (1998). Vpliv vіtamіnnih preparatіv na stіykіst tvarin do gіpoksіi [The effect of vitamin supplements on the resistance of animals to hypoxia]. Bulletin of the Odessa State University, 2, 136–140.

Rozanov, A. Ja., Treshhinskij, A. N., Hmelevskij, Ju. V. (1985). Fermentativnye processy i ih korrekcija pri jekstremal'nyh sostojanijah [Enzymatic processes and their correction in extreme conditions]. Kiev: Zdorov’ya, 208.

Lukyanova, L. D. (2000). Sovremennye problemy gipoksii [Modern problems of hypoxia]. Bulletin of RAMN, 9, 3–12.

Shilov, P. I., Jakovlev, F. M. (1974). Osnovy klinicheskoj vitaminologii [Fundamentals of Clinical vitaminology]. Leningrad, Russia: Medicine, 343.

Morozkina, T. S., Moiseenok, A. G. (2002). Vitaminy: Kratkoe ruk. dlja vrachej i studentov med., farmacevt. i biol. special'nostej [Vitaminy: Quick hands for doctors and med. students. pharmacist. and biol. specialties]. Minsk: Asar, 112.

Smirnov, M. I. (1974). Vitaminy [Vitamins]. Moscow: Medicine, 384–414.

Vinogradova, R. P. (1978). Molekuljarnye osnovy dejstvija fermentov [Molecular basis of enzyme activity]. Kiev: Vishha shkola, 280.

Chernadchuk, S. S., Petrov, S. A., Budnjak, O. K., Sorokіn, A. V., Jakimenko V. E., Kravchuk, І. O. (2015). Aktivnіst' amіnotransferaz v organah shhurіv pri gіpoksіi zamknenogo prostoru za dіeju tіamіnbromіdu [Aminotransferases activity in the organs of rats with hypoxia closed space by thiaminebromide action]. ScienceRise, 5/1 (10), 27–30. doi: 10.15587/2313-8416.2015.42749

Karkischenko, N. N. Rukovodstvo po laboratornym zhivotnym i al'ternativnym modeljam biomedicinskih tehnologij [Guide to laboratory animals and alternative models of biomedical technologies]. Moscow, 344.

Official Journal of the European Union L276 / 33. Directive 2010/63 / EU of the European parliament and of the council of 22 September 2010 on the protection of animals used for scientific purposes. 86/609 / EC.20.10.2010.

Prohorova, M. I. (1982). Metody biohimicheskih issledovanij (lipidnyj i jenergeticheskij obmen) [Metods of biochemical tests (lipid and energy metabolism)]. Leningrad State University Press, 272.

Glants, S. (1998). Mediko-biologicheskaja statistika [Biomedical Statistics]. Moscow: Practika, 459.

Normative-directive documents of Ministry of Public Health of Ukraine. Available at: http://mozdocs.kiev.ua/likiview.php?id=3119/

Kovalenko, V. N. (2014). Kompedium 2014 – lekarstvennye preparaty [Kompedium 2014 – drugs]. Kiev: Morion.

Published

2015-07-27

Issue

Section

Biological sciences