DOI: https://doi.org/10.15587/2313-8416.2015.47684

Molecular ang genetic analysis of polymorphous variants val158met of the comt gene in children who need dental treatment under the general ahesthesia

Галина Мироновна Солонько, Ніна Іванівна Смоляр, Марта Ярославівна Тиркус, Галина Василівна Макух

Abstract


The study of an influence of polymorphism of the СОМТ 2 gene on forming the negative emotions caused by the dental intervention allows to explain an appearance of a fear in children.

Aim. An analysis of polymorphous variants Val158Met of the СОМТ gene in children who underwent the dental treatment under the general anesthesia.   

Materials and methods. There were studied the patterns of DNA taken from 31 children who underwent the dental treatment under anesthesia and from 30 children who had not fear of dentist and were treated in usual conditions.  

Results. As the result of the molecular and genetic research of polymorphous locus 472 G>A (Val158Met) of the COMT gene in the group of children with an unconquerable fear of dental treatment comparing with the control one of children who agreed to cooperation were detected the probabilistic differences in distribution of genotypes and alleles (р=0,02). There was established that the presence of Met/Met genotype of the locus 472 G>A (Val158Met) of the COMT gene is a genetic factor that increase the risk of the development of the fear of treatment that can be explained by the higher pain sensitivity in children with such genotype. So the genetic test of the 472 G>A (Val158Met) locus of the COMT gene can be recommended to detect the groups of children that dental treatment under the general anesthesia can be recommended for.


Keywords


Children; dental treatment; fear; general anesthesia; molecular and genetic analysis; COMT gene; allelic polymorphism.

References


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GOST Style Citations


1. Sheller, B. Reasons for repeat dental treatment under general anesthesia for the healthy child [Text] / B. Sheller, B. J. Williams, K. Hays, L. Mancl // Pediatr Dent. – 2003. – Vol. 25, Issue 6. – P. 546–552

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6. Mc Pherson, M. J. PCR: a Practical Approash [Text] / M. J. Mc Pherson, P. Quirke, G. R. Taylor. – New York : Oxford University press, 1993. – 253 p.

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8. Clerget-Darpoux, F. Introduction to the genetic epidemiology of multifactorial diseases [Text] / F. Clerget-Darpoux, S. Lyonnet, P. Broet // ESHG COURSE. – CHU du Kremlin Bicertre, Faculte de Medecine Paris–Sud, France, 2009.

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