Mono- and combined antimicrobial agents efficiency in experimental wound infection
DOI:
https://doi.org/10.15587/2313-8416.2015.52300Keywords:
localized infection, Staphylococcus, necrosis, Ciprofloxacin, Decamethoxin, Hexamethylenetetramine, Phenyl salicylate, combined drugsAbstract
Modern problems of antibiotic therapy are shown by wide range of side effects, both on organism and microbiological levels: the spread of allergies, toxic for organ systems reactions, dysbiosis development, and resistant pathogens formation and dissemination. Therefore the necessity of search for new effective drugs with significant antimicrobial activity applied for the wounds treatment arises. Development of combined remedies on the background of different origin antimicrobial agents’ derivatives is one of the fight directions against infectious diseases in the skin pathology. Recently among the existing antimicrobial agents one should focus on antiseptic drugs, due to degenerative and dysfunctional effect on microbial cell.
Aim of research. The comparison of mono- and combined antimicrobial agents chemotherapeutic efficiency in the treatment of localized purulent infection under experimental conditions.
Metods. The study of chemotherapeutic efficiency was carried out on the model of localized purulent Staphylococcus infection on albino mice weighting 14 – 16 g. S.aureus ATCC 25923 strains were used as infectious agents. The contamination was performed subcutaneously to the right side of mice’s skin after depilation. The animals were randomly divided into 4 groups: the 1st group – infected mice without treatment (control); the 2nd group – infected mice treated with a ciprofloxacin; the 3rd group – infected mice treated with a Ciprofloxacin and Decamethoxin combination; the 4th group – infected mice treated with a combined drug on the base of mutual prodrugs (Hexamethylenetetramine and Phenyl salicylate).
Results. The efficiency of mono- and combined antimicrobial agents under experimental Staphylococcus wound infection conditions was studied. It was found that localized purulent staph center was formed more slowly in comparison with control and mono preparation use (2nd group of animals). The average index of skin lesions in comparison with control and 2nd groups’ data (33 and 24) amounted to 6,4 in the 3rd group and 6,2 in the 4th group per animal respectively. The use of combined remedies №1 and №2 accelerated the recovery periods at 2,2 and 2,8 times in comparison with control group.
Conclusion. It was found that the most effective treatment of animals with localized Staphylococcus infection was when used in Ciprofloxacin and Decamethoxin combination, and antiseptics with Salol-dependent principle of action combination.
It was proved that on the pre-clinical modeling of localized purulent infection level suggested combinations show satisfactory therapeutic and preventive properties
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