Elisa development for detection of glyphosat resistant gm soybean

Authors

  • Владислав Геннадійович Спиридонов National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine http://orcid.org/0000-0003-1197-0507
  • Ярослав Васильович Хоменко National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine http://orcid.org/0000-0002-7109-6797
  • Вадим Дмитрович Іщенко National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine http://orcid.org/0000-0002-8837-022X
  • Василь Сергійович Гончаренко National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine http://orcid.org/0000-0001-6643-5021
  • Наталія Миколаївна Шимко National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine http://orcid.org/0000-0002-5332-4311
  • Дмитро Юрійович Рибальченко National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine http://orcid.org/0000-0002-7460-4654

DOI:

https://doi.org/10.15587/2313-8416.2015.53844

Keywords:

genetically modified plants, enzyme immunoassay, 5-enolpyruvylshikimate-3-phosphate synthase, yolk antibodies

Abstract

During research we have utilized recombinant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS), conferring resistance to glyphosate for GM soybean, for the hen immunization and obtaining specific yolk antibodies IgY. Stages of ELISA development that can detect at least 0,1 % of GM-soybean resistant to glyphosate were present

Author Biographies

Владислав Геннадійович Спиридонов, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Doctor of Agricultural Sciences, deputy director

Ukrainian Laboratory of Quality and Safety of Agricultural Products

Ярослав Васильович Хоменко, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Postgraduate, Key specialist

Ukrainian Laboratory of Quality and Safety of Agricultural Products

Вадим Дмитрович Іщенко, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Candidate of veterinary science, associated professor

Department of pharmacology and toxicology

Василь Сергійович Гончаренко, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Senior Researcher

Ukrainian Laboratory of Quality and Safety of Agricultural Products

Наталія Миколаївна Шимко, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Senior Researcher

Ukrainian Laboratory of Quality and Safety of Agricultural Products

Дмитро Юрійович Рибальченко, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Researcher

Ukrainian Laboratory of Quality and Safety of Agricultural Products

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Published

2015-11-27

Issue

Section

Biological sciences