Prevalence of TLR-4 gene polymorphism in patients co-infected with HIV/HCV
Keywords:HIV|HCV co-infection, Toll-like receptors, metabolic disorders, cytokines, lipid metabolism, insulin-resistance
There was studied the prevalence of Asp299Gly polymorphism of TLR-4 gene in patients co-infected with HIV-HCV.
Methods. To define the frequency of Asp299Gly polymorphism of TLR-4 gene there were examined 47 patients co-infected with HIV\HCV and 30 healthy donors by the method of polymorphism of the long restrictive fragments (PLRF) – polymerase chain reaction (PCR). Among examined persons there were 21 (44,7 %) women, 26 (55,3 %) – men. The mean age was 36,2±2,4 years, from 21 to52 years old.
Results. The correlative analysis indicates the reverse dependency between Asp299Gly polymorphism of TLR-4 gene and Zn content (r=-0,34; p<0,05), relative numder of CD4+,% (r=-0,32; p<0,05) in blood serum of patients co-infected with HIV\HCV. At the same time in patients co-infected with HIV\HCV there was noticed the direct dependency between Asp299Gly polymorphism of TLR-4 gene and α tumor necrosis factor (r=0,32; p<0,05), С-reactive protein (r=0,34; p<0,05), absolute number of СD3+ Т-lymphocytes (r=0,34; p<0,05), TG level (r=0,39; p<0,02), absolute numder of CD45+ Т-lymphocyte (r=0,45; p<0,001), insulin level (r=0,66; p<0,001) and insulin-resistance (r=0,66; p<0,001).
Conclusions: In patients co-infected with HIV\HCV the Asp299Gly polymorphism of TLR-4 gene is reliably more often than the control group (χ2=4,5; р<0,05). The correlative analysis demonstrated the powerful direct dependency between Asp299Gly polymorphism of TLR-4 gene and clinical stage of disease in patients co-infected with HIV\HCV (r=0,43; p<0,001)
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