Peculiarities of tropane alkaloids determination in Datura Stramonium L. leaves

Authors

  • Володимир Анатолійович Міщенко National University of Pharmacy 53 Pushkinska str., Kharkiv, Ukraine, 61002, Ukraine
  • Юлія Сергіївна Прокопенко National University of Pharmacy 53 Pushkinska str., Kharkiv, Ukraine, 61002, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.56940

Keywords:

standardization, monograph, natural extracts, Datura stramonium, tropane group of alkaloids

Abstract

The questions of the search, pharmacological activity and standardization of natural extracts are appropriate and important for national pharmaceutical science and practice. Since 2000, one of the key points of the Action Plan to ensure the integration of Ukraine into the European Union is development of the State Pharmacopoeia of Ukraine harmonized with the European Pharmacopoeia (PhEur).

Aim: A comparative analysis of herbal material according to the methods for tropane group alkaloids determination, as described in the PhEur and in the 11th Edition of USSR Pharmacopoeia Monographs «Stramonium leaf».

Methods: Datura leaves samples have been harvested during 2010-2012 in different regions of Ukraine for experimental research. Identification of tropane group alkaloids was carried out by the method of thin-layer chromatography (TLC) and by Vitali-Morin colour reaction. According to the PhEur method, firstly Dragendorff reagent and then Sodium nitrite solutions were used for the TLC plates spraying. Alkaloids assay was carried out by alkalimetry method with indicator determination of equivalence point.

Results: Datura leaves qualitative features, defined by PhEur and the 11th Edition of USSR Pharmacopoeia Monographs «Stramonium leaf», as well as their rationing have been analyzed. Certain differences concerning regulated quality parameters of herbal material have been determined. A comparative analysis of the natural extracts by described in the given normative documents methods for determination of tropane group alkaloids content has been done. After spraying the TLC plates with Sodium nitrite solution, red-brown zones corresponding Hyoscyamine were determined. Hyoscine zones were characterized by less intense color. As a result of quantitative determination it was determined that the alkaloids content in analyzed Datura leaves samples was within the limits regulated by the PhEur and the 11th Edition of USSR Pharmacopoeia – more than 0,25% calculated as atropine.

Conclusion: Having analyzed the assay methods, described in the PhEur and the 11th Edition of USSR Pharmacopoeia Monographs «Stramonium leaf», applied for the determination of tropane group alkaloids in Datura leaves, certain differences in tests preparation were determined. Nevertheless, both of the methods allow determination of tropane group alkaloids content in Datura leaves samples. The obtained experimental data are relevant and were considered when developing the State Pharmacopoeia of Ukraine Monograph «Stramonium leaf»

Author Biographies

Володимир Анатолійович Міщенко, National University of Pharmacy 53 Pushkinska str., Kharkiv, Ukraine, 61002

PhD, Senior lecturer

Department of Quality, Standardization and Certification of Medicines

Юлія Сергіївна Прокопенко, National University of Pharmacy 53 Pushkinska str., Kharkiv, Ukraine, 61002

PhD, associate professor

Department of Quality, Standardization and Certification of Medicines

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Published

2015-12-29

Issue

Section

Pharmaceutical Sciences