Comparative polymorphism of sterlet fertilizers (Acipenser Ruthenus) for microsatellite DNA markers

Authors

  • Ольга Олексіївна Малишева Ukrainian laboratory of quality and safety of agricultural products 7 Mashinostroiteley str., Shepherds, Kiev Svyatoshinsky district, Kiev region., Ukraine, 08162, Ukraine
  • Христина Миколаївна Курта National University of Life and Environmental Sciences of Ukraine 15 Heroes of Defense str., Kyiv, Ukraine, 03041, Ukraine
  • Любов Іванівна Калакайло National University of Life and Environmental Sciences of Ukraine 15 Heroes of Defense str., Kyiv, Ukraine, 03041, Ukraine
  • Лариса Миколаївна Шинкаренко Ukrainian laboratory of quality and safety of agricultural products 7 Mashinostroiteley str., Shepherds, Kiev Svyatoshinsky district, Kiev region., Ukraine, 08162, Ukraine
  • Владислав Геннадійович Спиридонов Ukrainian laboratory of quality and safety of agricultural products 7 Mashinostroiteley str., Shepherds, Kiev Svyatoshinsky district, Kiev region., Ukraine, 08162, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.54039

Keywords:

sturgeons, sterlet, aquaculture, fertilizers, microsatellite DNA markers, locus, allele, genotype, polymorphism, selection of parental pairs

Abstract

Based on microsatellite DNA markers in three (LG-19, LS-68 and LS-39) it is examined intraspecific genetic structure of sterlet fertilizers. As a result of this work it was found that this group of fish is in a balanced state in terms of the genetic polymorphism. On the basis of certain individual differences in allelic variants have been selected and combined the parental pairs for alternative genotypes. The results of the research allow optimize further work on the reproduction of sturgeon under artificial cultivation

Author Biographies

Ольга Олексіївна Малишева, Ukrainian laboratory of quality and safety of agricultural products 7 Mashinostroiteley str., Shepherds, Kiev Svyatoshinsky district, Kiev region., Ukraine, 08162

researcher of molecular diagnostic tests

Христина Миколаївна Курта, National University of Life and Environmental Sciences of Ukraine 15 Heroes of Defense str., Kyiv, Ukraine, 03041

Postgraduate

Department of breeding, breeding and animal reproductive biotechnologies

Любов Іванівна Калакайло, National University of Life and Environmental Sciences of Ukraine 15 Heroes of Defense str., Kyiv, Ukraine, 03041

Department of Ichthyology

Лариса Миколаївна Шинкаренко, Ukrainian laboratory of quality and safety of agricultural products 7 Mashinostroiteley str., Shepherds, Kiev Svyatoshinsky district, Kiev region., Ukraine, 08162

specialist of molecular diagnostic tests

Владислав Геннадійович Спиридонов, Ukrainian laboratory of quality and safety of agricultural products 7 Mashinostroiteley str., Shepherds, Kiev Svyatoshinsky district, Kiev region., Ukraine, 08162

Doctor of Agricultural Sciences Agricultural Sciences

Head of the department of molecular diagnostic tests

References

Chelomina, G. N., Rozhkovan, K. V., Ivanov, S. A. (2008). Diskriminatsiya mezhvidovykh gibridov v prirodnykh populyatsiyakh osetrovykh ryb amura s pomoshch'yu mul'tilokusnykh rapd_pcr markerov. Tsitologiya i genetika, 5, 61–71.

Barannikova, I. A., Nikonorov, S. I., Belousov, A. N. (2000). Problema sokhraneniya osetrovykh v sovremennyy period. Osetrovye na rubezhe KhKhІ veka. Astrakhan', 7–9.

Tretjak, O. M., Ghankevych, B. O., Kolos, O. M., Jakovljeva, T. V. (2010). Stan zapasiv osetrovykh ryb ta rozvytok osetrovoji akvakuljtury v Ukrajini. Ryboghospodarsjka nauka Ukrajiny, 4, 4–22.

Malysheva, O. O., Spyrydonov, V. Gh., Meljnychuk, S. D. (2014). Ghenetychna struktura populjaciji sterljadi (Acipenser ruthenus) za mikrosatelitnymy markeramy DNK. Visnyk sumsjkogho nacionaljnogho aghrarnogho universytetu: Serija «Tvarynnyctvo», 2/1 (24), 212–215.

Malysheva, O. O., Spyrydonov, V. Gh., Meljnychuk, S. D., Kurta, Kh. M. (2014). Vykorystannja mikrosatelitnykh DNK-markeriv dlja pidboru ta kombinuvannja par plidnykiv sterljadi ta bestera. Naukovo-tekhnichnyj bjuletenj Instytutu Biologhiji tvaryn i DMDKI vetpreparativ ta kormovykh dobavok, 15 (2, 3), 245–251.

Barmintseva, A. E., Myuge, N. S. (2013). Ispol'zovanie mikrosatellitnykh lokusov dlya ustanovleniya vidovoy prinadlezhnosti osetrovykh (Acipenseridae) i vyyavleniya osobey gibridnogo proiskhozhdeniya. Genetika zhivotnykh, 49 (9), 1093–1105.

Lesyuk, M. I., Koneva, O. Yu., Rovba, E. A., Slukvin, A. M. (2012). Molekulyarno-geneticheskie issledovaniya proizvoditeley sterlyadi. Molekulyarnaya i prikladnaya genetika, 13, 110–117.

Dudu, A. A., Georgescu, S. E., Burcea, A. Florencu, I., Costahe, M. (2013). Microsatelittes Variation in Sterlet Sturgeon, Acipenser Ruthenus from the Lower Danube . Animal Science and Biotechnologies, 46 (1), 90–94.

Kozlova, N. V., Bazelyuk, N. N., Fayzulina, D. R., Stonogina, E. V. (2013). Primenenie molekulyarno-geneticheskikh issledovaniy v akvakul'ture osetrovykh ryb. Vestnik AGTU. Seriya: Rybnoe khazyaystvo, 3, 113–117.

Slukvin, A. M., Koneva, O. Yu., Lesyuk, M. I. (2009). Rezul'taty populyatsionnoy identifikatsii proizvoditeley sterlyadi (Acipenser ruthenus) OAO «Rybkhoz»Poles'e» (Brestskaya oblast', Belarus'), poluchennye s pomoshch'yu mikrosatellitnogo analiza DNK. Pervaya konferentsiya molodykh uchenykh NACEE. Voprosy akvakul'tury, 46–47.

May, B., Krueger, C. C., Kincaid, H. L. (1997). Genetic variation at microsatellite loci in sturgeon: primer sequence homology in Acipenser and Scaphirhynchus. Canadian Journal of Fisheries and Aquatic Sciences, 54 (7), 1542–1547. doi: 10.1139/cjfas-54-7-1542

Boom, R., Sol, C., Salimans, M. et. al. (1990). Rapid and Simple Method for Purification of Nucleic Acids. Journal of clinical microbiology, 28, 495–503.

Rjeznykova-Ghalashevych, I. S., Stepura, V. V., Sheljov, A. V., Spyrydonov, V. Gh., Tabaka, P. P., Meljnychuk, S. D., Alymov, S. I. (2011). Ghenetychna identyfikacija promyslovykh vydiv ryb. Kyiv: Vydavnychyj centr NUBiP Ukrajiny, 35.

Kalinowski, S. T., Taper, M. L., Marshall, T. C. (2007). Revising how the computer program cervus accommodates genotyping error increases success in paternity assignment. Molecular Ecology, 16 (5), 1099–1106. doi: 10.1111/j.1365-294x.2007.03089.x

Marshall, T. C., Slate, J., Kruuk, L. E. B., Pemberton, J. M. (1998). Statistical confidence for likelihood-based paternity inference in natural populations. Molecular Ecology, 7 (5), 639–655. doi: 10.1046/j.1365-294x.1998.00374.x

Published

2015-11-24

Issue

Section

Agriculture