Application of cytogenetic researches in efficiency assessment of vaccines against leukemia virus in cattle

Authors

DOI:

https://doi.org/10.15587/2313-8416.2018.121096

Keywords:

genetic monitoring, cattle leukemia virus, immunogenesis, mice, bone marrow cells

Abstract

We studied the effect of immunogens of the leukemia virus in cattle on the cytogenetic parameters of bone marrow in the dynamics of immunogenesis. We found that each drug has its own specific effect, which is due to the structural differences of the leukemia virus in cattle. The method of cytogenetic studies of bone marrow cells should be used to assess the safety and immunogenic properties of retroviral origin drugs

Author Biographies

Maria Drahulian, National University of Life and Environmental Sciences of Ukraine Heroiv oborony str., 15, Kyiv, Ukraine, 03041

PhD, Researcher

Department of Genetics, Breedingand Biotechnology Animals

Tetiana Dorosch, National University of Life and Environmental Sciences of Ukraine Heroiv oborony str., 15, Kyiv, Ukraine, 03041

PhD, Assistant

Department of Therapy and Clinical Diagnosis

Svitlana Kostenko, National University of Life and Environmental Sciences of Ukraine Heroiv oborony str., 15, Kyiv, Ukraine, 03041

Doctor of Biological Sciences, Professor

Department of Genetics, Breeding and Biotechnology Animals

References

Fedorova, N. E., Medzhidova, А. А., Medzhidova, М. G., Kusch, А. А. (2003). Blok kletochnoy proliferatsii i patologii mitoza v kletkakh infitsirovannykh tsitomegalovirusom [Cell proliferation blocking and mitosis pathology in cells infected by cytomegalovirus]. Academy of science abstracts, 392 (4), 552–555.

Cerkhezia, S. E., Mykhaylova, G. R., Gorshunova, L. P. (1980). Issledovaniye khromosom v kletkakh kostnogo mozga myshey. immunizirovannykh antirabicheskimi vaktsinami [Chromosome research of bone marrow cells of mice which took anti rabies vaccine]. Cytology and genetics, 14 (4), 67–70.

Il’inskykh, I. N., Novitsky, V. V., Il’inskykh, E. N., Il’inskykh, N. N., Tkachenk, S. B. (2005). Infektsionnaya kariopatologiya [Infectious caryopathology]. Tomsk: Tomsk university press, 168.

Yarylin, А. А. (2003). Apoptoz i ego rol v tselostnom organizme [Apoptosis and its role in an organism as a whole]. Glaucoma, 2, 46–54.

Serenot, Ch. K. (2005). Izucheniye izmeneniy morfologii yadra kultury kletok k 562 pod vozdeystviyem vaktsin virusov [Studies related to morphological changes k-562 cell culture nuclei influenced by viral vaccine]. Thesis collection related to the topics of 64-th International scientific students’ conference named after N. I. Pirogov, 52.

Sivakova, T. N., Mylnikova, E. A. (2010). Kariopaticheskoye deystviye vaktsiny Nobivak Rabies na kletki kostnogo mozga i semennikov belykh myshey [Caryopathic effect of Nobivak Rabies vaccine on bone marrow cells and white mice testis]. Fundamental medical-biological science and practical healthcare, 46–49.

Yakovlev, A. F., Stefanova, V. N., Rossoha, V. I., Yefimenko, L. I. (1990). Metodicheskiye rekomendatsii po tsitogeneticheskomu testirovaniyu v svinovodstve [Methodological guidance on cytogenetic testing in swine breeding]. Kharkiv: Ukraine Veterinary Theoretical research institute, 24.

Brodbeck, W. G., Shive, M. S., Colton, E., Ziats, N. P., Anderson, J. M. (2002). Interleukin-4 inhibits tumor necrosis factor-α–induced and spontaneous apoptosis of biomaterial-adherent macrophages. Journal of Laboratory and Clinical Medicine, 139 (2), 90–100. doi: 10.1067/mlc.2002.121260

Saito, T., Kuss, I., Dworacki, G., Gooding, W., Johnson, J. T., Whiteside, T. L. (1999). Spontanous ex vivo apoptosis of peripheral blood mononuclear cells in patients with head and neck cancer. Clinical Cancer Research, 5, 1263–1273.

Il’inskykh, N. N. (1984). Tsitogenenticheskiy analіz posledstviy infektsionnogo mutageneza v svyati s sostoyaniyem immunoreaktivnosti organizma [Cytogenetic analysis of consequences of infectious mutagenesis complimented by condition of immunoreactivity of an organism]. Tomsk, 42.

Ingel, F. I. (2006). Perspectives of micronuclear test in human lymphocytes cultivated in cytogenetic block conditions. Part 1: Cell proliferation. Ecological Genetics, 4 (3), 7–19. doi: 10.17816/ecogen437-19

Ilin, D. A. (2006). Aspekty formirovaniya mikroyader [Formation of micronuclei]. Natural science and humanism, 3 (3), 58.

Yarylin, А. А. (1996). Apoptoz i ego mesto v immunnykh protsessakh [Apoptosis and its role in immune processes]. Immunology, 6, 10–23.

Shimizu, N., Kamezaki, F., Shigematsu, Sh. (2005). Tracking of microinjected DNA in live cells reveals the intracellular behavior and elimination of extrachromosomal genetic material. Nucleic Acids Research, 33 (19), 6296–6307. doi: 10.1093/nar/gki946

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Published

2018-01-16

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Section

Veterinary science