Physiological aspects of rat activity, their anxiety and memory after administration of full gabaa-receptor complex agonist propoxazepam

Authors

  • Mykola Golovenko A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080, Ukraine https://orcid.org/0000-0003-1485-128X
  • Igor Belenichev Zaporizhia State Medical University Maiakovskoho ave., 26, Zaporizhia, Ukraine, 69035, Ukraine https://orcid.org/0000-0003-1273-5314
  • Vitalii Larionov A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080, Ukraine https://orcid.org/0000-0003-2678-4264
  • Anatoliy Reder “INTERCHEM” SLC Liustdorfska doroha str., 86, Odessa, Ukraine, 65080 A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080, Ukraine https://orcid.org/0000-0002-1801-8378
  • Andronati Serhiy A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080, Ukraine https://orcid.org/0000-0001-8451-6327

DOI:

https://doi.org/10.15587/2519-8025.2020.207368

Keywords:

propoxazepam, locomotor and exploratory activity, reference and working memory

Abstract

The action of 7-bromo-5 (o-chlorophenyl) -3-propoxy-1,2-dihydro-3H-1,4-benzodiazepin-2-one (propoxazepam) with prolonged administration in various doses on behavioural reactions, anxiety and the memory of rats, as well as their muscle tone was estimated, which is important paying attention to its main (anticonvulsant and analgesic) pharmacological effects.

The aim of the study was a comparative assessment of the severity and duration of propoxazepam effect after its administration to rats at doses of 2, 5 and 10 mg/kg (10 days) on higher functions of the central nervous system according to indicators such as motor and exploratory activity, as well as reference and working memory .

Materials and methods. The study was conducted on 40 Wistar rats weighing 220-290 g. The psychophysiological state of the animals was evaluated using the "open field" test, the formation of spatial working and long-term memory in a radial eight-arm maze, muscle relaxation, imbalance and movements coordination - using the "rotating rod" method. Statistical processing was performed in Microsoft Excel 2016 with AtteStat 12.

The results of the study. When comparing the test results of the "open field", "radial labyrinth" and "rotarod" in groups of animals that were administered different doses of the compound, it was found that, in general, these parameters at doses of 2 mg/kg and 5 mg/kg statistically significantly different (increase) from control values, but are similar. At a dose of 10 mg/kg, most of the parameters (except for the rotarod test) for these animals were characterized by a downward trend.

Conclusions. The administration of the drug in doses of 2, 5 and 10 mg/kg leads to a decrease in anxious behaviour of animals, which is also accompanied by a pronounced dose-dependent negative effect on the endurance, coordination and memory of animals. The administration of the drug at a dose of 10 mg/kg impaired the learning ability of animals and reduced memory function. This should be considered in clinical studies of the compound

Author Biographies

Mykola Golovenko, A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080

Doctor of Biological Sciences, Professor, Chief Researcher, Academician of National Academy of Medical Sciences of Ukraine

Laboratory of Physico-Chemical Pharmacology

Igor Belenichev, Zaporizhia State Medical University Maiakovskoho ave., 26, Zaporizhia, Ukraine, 69035

Doctor of Biological Sciences, Professor, Head of Department

Department of Pharmacology and Medical Prescriptions

Vitalii Larionov, A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080

Doctor of Biological Sciences, Head of Laboratory

Laboratory of Physical-Chemical Pharmacology

Anatoliy Reder, “INTERCHEM” SLC Liustdorfska doroha str., 86, Odessa, Ukraine, 65080 A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080

PhD, General Director

Senior Researcher

Andronati Serhiy, A. V. Bogatsky Physical-Chemical Institute of National Academy of Sciences of Ukraine Liustdorfska doroha str., 86, Odessa, Ukraine, 65080

Doctor of Chemical Sciences, Professor, Head of Department, Academician of National Academy of Sciences of Ukraine

Department of Medicinal Chemistry

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Published

2020-04-30

How to Cite

Golovenko, M., Belenichev, I., Larionov, V., Reder, A., & Serhiy, A. (2020). Physiological aspects of rat activity, their anxiety and memory after administration of full gabaa-receptor complex agonist propoxazepam. ScienceRise: Biological Science, (2 (23), 42–48. https://doi.org/10.15587/2519-8025.2020.207368

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Biological Sciences