Morphological and anatomical properties of Veronica crista-galli Steven




Veronica crista-galli Stev., leaf, flower, fruit


The aim of this work was to study of diagnostic signs of the morphological and anatomical structure of Veronica crista-galli Steven. from the flora of Azerbaijan.

Materials and methods. The samples for research were collected during their flowering time in June 2018, in the Ismailli region of the Republic of Azerbaijan. Plant samples were fixed in a solution made in 0.1 M phosphate buffer (pH=7.4), containing 2.5 % glutar-aldehyde, 2.5 % paraformal-aldehyde and 0.1 % picric acid. In the next stage was the preparation of block and their filling in Araldite – Epon according to the TEM method.

Results. The leaf is simple, lower part is short-petiolate and upper is sessile. The surface, on both sides of the leaf, is reliefly, and 7–8 conductive veins are clearly visible. The lower and upper sides of the leaf, and also margin, are strewn with multicellular hairs. The calyx of the flower consists of two sepals which grown together at the base, covered with simple multicellular hairs. The stalk in is a long filiform. The corolla of flower consists of 4 petals which grown together at the base and 2 stamens attached to the tube of the corolla. On the epidermis, cells with sinuous and bead-like walls, numerous stomata of the stavrocytic type, capitate hairs are visible. From the cross section of the leaf, it is visible that palisade tissue at the upper and sponge tissue at the bottom.

Conclusions. As a result of morphological and anatomical studies, it was revealed that diagnostic signs of plant raw material can be: Present of multicellular hairs on the leaf blade; The location of the capsule between the sepals; Stavrocytic type of the stoma structure; The bead-like walls of the epidermis; Capitate hairs on the epidermis; Sepals covered by hairs.

The established anatomical diagnostic features can be used for the drafting of the normative document on the plant raw materials and for identification of plant raw material of Veronica crista-galli

Author Biographies

Nigar Hidayet Pashayeva, Azerbaijan Medical University

PhD Student

Department of Pharmaceutical Chemistry

Tahir Abbasali Suleymanov, Azerbaijan Medical University

Professor, Head of Department

Department of Pharmaceutical Chemistry

Yusif Balakerim Kerimov, Azerbaijan Medical University

Professor, Scientific Adviser

Department of Pharmacognosy

Eldar Kocheri Gasimov, Azerbaijan Medical University

Professor, Head of Department

Department of Histology, Embryology and Cytolgy

Fuad Huseynali Rzayev, Azerbaijan Medical University

Senior Researcher

Laboratory of Electron Microscopy


Kerimov, Yu., Suleimanov, T. (2016). Na sobstvennoi syrevoi baze. Vestnik Natsionalnoi Akademii Nauk Azerbaidzhana, 19–22.

Akanda, M. R., Nam, H.-H., Tian, W., Islam, A., Choo, B.-K., Park, B.-Y. (2018). Regulation of JAK2/STAT3 and NF-κB signal transduction pathways; Veronica polita alleviates dextran sulfate sodium-induced murine colitis. Biomedicine & Pharmacotherapy, 100, 296–303. doi:

Dunkić, V., Kosalec, I., Kosir, I., Potocnik, T., Cerenak, A., Koncic, M. et. al. (2015). Antioxidant and antimicrobial properties of Veronica spicata L. (Plantaginaceae). Current Drug Targets, 16 (14), 1660–1670. doi:

Saracoglu, I., Oztunca, F. H., Nagatsu, A., Harput, U. S. (2011). Iridoid content and biological activities ofVeronica cuneifoliasubsp.cuneifoliaandV. cymbalaria. Pharmaceutical Biology, 49 (11), 1150–1157. doi:

Sun, Y., Lu, Q., He, L., Shu, Y., Zhang, S., Tan, S., Tang, L. (2017). Active Fragment ofVeronica ciliataFisch. Attenuates t-BHP-Induced Oxidative Stress Injury in HepG2 Cells through Antioxidant and Antiapoptosis Activities. Oxidative Medicine and Cellular Longevity, 2017, 1–11. doi:

Yin, L., Lu, Q., Tan, S., Ding, L., Guo, Y., Chen, F., Tang, L. (2016). Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata. Chemistry Central Journal, 10 (1). doi:

Kroflič, A., Germ, M., Golob, A., Stibilj, V. (2018). Does extensive agriculture influence the concentration of trace elements in the aquatic plant Veronica anagallis-aquatica ? Ecotoxicology and Environmental Safety, 150, 123–128. doi:

Saracoglu, I., Harput, U. S. (2011). In Vitro Cytotoxic Activity and Structure Activity Relationships of Iridoid Glucosides Derived from Veronica species. Phytotherapy Research, 26 (1), 148–152. doi:

Süleymanov, T. A., Paşayeva, N. H., Stev, V. P., Stev, V. C.-G. (2018). Xammalında spektrofotometrik üsulla flavonoidlərin miqdari təyini. Azərbaycan təbabətinin müasir nailiyyətləri, 8, 121–123.

Saracoglu, I., Suleimanov, T., Pashaeva, N., Dogan, Z., Inoue, M., Nakashima, K. (2020). Iridoids from Veronica crista-galli from the Flora of Azerbaijan. Chemistry of Natural Compounds, 56 (4), 751–753. doi:

Kuo, J. (2007). Electron microscopy: methods and protocols. Totowa: Humana Press, 625.

Agayeva, N. J., Rzayev, F. H., Gasimov, E. K., Mamedov, C. A., Ahmadov, I. S., Sadigova, N. A. et. al. (2020). Exposure of rainbow trout (Oncorhynchus mykiss) to magnetite (Fe3O4) nanoparticles in simplified food chain: Study on ultrastructural characterization. Saudi Journal of Biological Sciences, 27 (12), 3258–3266. doi:

AL-Abide, N. M. (2019). A morphological and anatomical comparative Study of the Reproductive parts of the genusVeronicaL. (Plantaginaceae) in northern Iraq. Journal of Physics: Conference Series, 1294, 062114. doi:

Kaplan, A., Hasanoglu, A., Agah Ince, I. (2006). Morphological, Anatomical and Palynological Properties of Some Turkish Veronica L. Species (Scrphulariaceae). International Journal of Botany, 3 (1), 23–32. doi:




How to Cite

Pashayeva, N. H., Suleymanov, T. A., Kerimov, Y. B., Gasimov, E. K., & Rzayev, F. H. (2021). Morphological and anatomical properties of Veronica crista-galli Steven. ScienceRise: Biological Science, (3 (28), 15–20.



Biological research