https://journals.uran.ua/sr_bio/issue/feedScienceRise: Biological Science2024-02-19T19:58:59+02:00Yuliia Nikolaievabio@entc.com.uaOpen Journal Systems<p>«ScienceRise: Biological Science» – scientific peer-reviewed journal, published 4 times a year, included in category “B” «List of scientific professional editions of Ukraine» for specialty 091-Biology (Сertificated by order of Ministry of Education and Science of Ukraine No. 612 from 07.05.2019) and for specialty 211- Veterinary medicine (Сertificated by order of Ministry of Education and Science of Ukraine No. 320 from 07.04.2022).</p> <p style="font-weight: 400;">The goal of "ScienceRise: Biological Science" journal is to provide a platform for scientists to share scientific data in all aspects of the life sciences.</p> <p style="font-weight: 400;">The concept of "Ecosystems", which is considered from three points of view, is at the center of the researched issues of the journal:</p> <p style="font-weight: 400;">– biota, which is affected by external factors caused by human activity,<br />– the influence of environmental factors on the health of people and animals,<br />– medicinal properties of plants for their use in medicinal preparations.</p> <p style="font-weight: 400;">Such a focus of research should cover applied aspects related to human and animal health: identification of the causes of diseases and diagnosis based on microbiological studies, prevention, effects of medicinal drugs and supplements, biotechnological solutions for improving the state of health care and the environment.</p> <p style="font-weight: 400;">Therefore, the areas of scientific research covered in the "ScienceRise: Biological Science" journal are divided as follows:</p> <p style="font-weight: 400;">Life Sciences:</p> <ul> <li style="font-weight: 400;">Biological and agricultural sciences (plants that have medicinal properties);</li> <li style="font-weight: 400;">Biochemistry, genetics and molecular biology (biochemistry, biotechnology);</li> <li style="font-weight: 400;">Environmental science (ecology, health, toxicology and mutagenesis);</li> <li style="font-weight: 400;">Immunology and microbiology (applied microbiology and biotechnology, immunology, parasitology, virology).</li> </ul> <p style="font-weight: 400;">Health Sciences</p> <ul> <li style="font-weight: 400;">Pharmacology, toxicology and pharmaceuticals;</li> <li style="font-weight: 400;">Veterinary and veterinary medicine.</li> </ul> <p><a href="https://portal.issn.org/resource/ISSN/2519-8017">ISSN 2519-8017 </a> (print), <a href="https://portal.issn.org/resource/ISSN/2519-8025">ISSN 2519-8025 </a>(on-line) <br /><br />Drawing up the items of the publication ethics policy of the journal «ScienceRise: Biological Science» Editors followed the recommendations of Committee on Publication Ethics<a href="http://publicationethics.org/"> (COPE)</a>.</p>https://journals.uran.ua/sr_bio/article/view/298576Problems of collecting genetic history, interpretation of pedigrees with common and rare dermatological, skeletal, dental and other peculiarities that affect the appearance and health of a human2024-02-16T13:16:54+02:00Olga Filiptsovaphiliptsova@yahoo.comOlga Nabokaolganaboka2012@gmail.comOleg Kryskivoleg.kryskiw@gmail.comAndriy Zakharievvagabond19973@gmail.comTetiana Martyniuktat.martynyuk@gmail.comNatalia Khokhlenkovahohnatal@gmail.comOlha Kaliuzhnaiakalyuzhnayao.s@gmail.comSvitlana Bobrosvetabobro1@gmail.comLiudmyla Petrovskal.s.petrovskaya96@gmail.comYuliia Osypenkojulie.osipenko17@gmail.com<p><strong>The aim</strong> of the study was to analyze the problem of constructing and interpreting human pedigrees, which include some signs that are easily detected during a clinical examination and the collection of primary history.</p> <p><strong>Materials and methods.</strong> 2 pedigrees from own sources and 2 pedigrees of famous people from literary sources are presented. They serve as the examples of using the provided data for further medical and genealogical analysis.</p> <p><strong>Results and discussion.</strong> The results indicate that pedigree analysis is a primary and informationally important tool for the further algorithm of diagnosis and treatment of subjects. The effect of the quantity, quality and testing level of the presented genealogical information for a full pedigree analysis and the use of its possibilities is demonstrated.Pedigree analysis demonstrated that the number of absolute units of quantitative information presented (total number of generations and members of the pedigree) and absolute units of qualitative information (traits/medical conditions listed and their level of detail) may not be associated with the value of genetic conclusions. It has been shown that the largest number of members in a generation is usually represented for the generation of the propositus.Thus, smaller pedigrees, where more units of information are represented per member, can be of greater value for genetic conclusions. An analysis of one's own pedigrees showed that, on average, less than one trait can be represented per person in the pedigree. Additionally, small pedigrees with available molecular genetic data may be of greatest value, even retrospectively.</p> <p><strong>Conclusions.</strong> It is shown that pedigrees can represent valuable primary information for clinicians regarding the further algorithm of the subject dealing and serve as a tool for the scientific thinking development in the educational process</p>2023-12-29T00:00:00+02:00Copyright (c) 2024 Olga Filiptsova, Olga Naboka, Oleg Kryskiv, Andriy Zakhariev, Tetiana Martyniuk, Natalia Khokhlenkova, Olha Kaliuzhnaia, Svitlana Bobro, Liudmyla Petrovska, Yuliia Osypenkohttps://journals.uran.ua/sr_bio/article/view/298594Evaluation of antimicrobial activity of biomaterials based on alginate and decametoxin against Staphylococus aureus and Escherichia coli2024-02-16T16:42:33+02:00Oleksandr Nazarchuknazarchukoa@gmail.comTetyana Denyskotanya.denisko@gmail.com<p><strong>The aim</strong> was to study the antimicrobial activity of new biomaterials based on decamethoxine and commercially available wound dressings against reference and clinical strains of S.aureus and E.coli.</p> <p><strong>Materials and methods.</strong> Developed biomaterials with 0.05 % decamethoxine (DCM No. 1-3) and wound dressings containing antiseptics Suprasorb®, SILVERCEL®, Urgotul SSD®, GUANPOLISEPT®, Bétadine® were used for the study. Antimicrobial properties were studied by zone of inhibition (ZOI) testing using the Kirby-Bauer method.</p> <p><strong>Results. </strong>In relation to S.aureus ATCC 25923, a significantly higher antimicrobial activity of biomaterials with DCM compared to silver- and iodine-containing wound dressings was found to be 1.97-2.11 (p <0.001) and 1.73-1.86 times (p <0.001), respectively. Similar activity against S.aureus ATCC 25923 was possessed by all three samples with DCM (ZOI - from 21.98±0.18 to 23.58±0.26 mm) and Suprasorb® (19.31±0.17 mm), Guanpolisept® (19.13±0.12 mm). Such a tendency was also found in relation to clinical strains of staphylococci. A high level of activity against E.coli ATCC 25922 was shown by biomaterials No. 1-3 DCM (ZOI - from 19.01±0.33 to 21.54±0.23 mm), Guanpolisept® (18.74±0.12 mm) and Suprasorb® (18.43±0.13 mm). Clinical strains of E.coli showed greater tolerance to antimicrobial biomaterials: the difference in mean values between the ZOI of the reference and ZOI of clinical strains of E.coli was significant for all biomaterials (p <0.001). The most effective were biomaterials with DCM No. 1-3 (ZOI - from 15.58±0.25 to 16.41±0.16 mm), as well as Suprasorb® (15.82±0.31 mm).</p> <p><strong>Conclusions.</strong> Biomaterials based on decamethoxine No. 1, No. 2, No. 3, Suprasorb®, Guanpolisept®, and Bétadine® have the highest antistaphylococcal activity. Biomaterials with decamethoxin No.1-3, Suprasorb® and Guanpolisept® show the strongest effect on reference and clinical strains of E.coli</p>2023-12-29T00:00:00+02:00Copyright (c) 2024 Oleksandr Nazarchuk, Tetyana Denyskohttps://journals.uran.ua/sr_bio/article/view/298736Blood plasma proteins and the content of metabolites of carbohydrate and lipid metabolism in the hepatopancreas of fish during the action 19-nortestosterone2024-02-19T19:58:59+02:00Mykola Zakharenkosangin1996@ukr.netElla Romanovaromanova_ella@ukr.net<p><strong>The aim:</strong> study the effect of the synthetic anabolic steroid 19-nortestosterone on the fractional composition of blood plasma proteins, as well as the total protein and albumin content, indicators of the metabolism of carbohydrates, lipids, macro- and microelements in the fish hepatopancreas.</p> <p><strong>Materials and methods.</strong> The experiments were conducted on two-year-old carp (Cyprinus carpio L.), which were kept for 24 hours in aquariums with a volume of 40 dm<sup>3</sup> with different concentrations of 19-nortestosterone in water at optimal temperature, oxygen content, and pH. Spectrophotometric methods and an automatic biochemical analyzer were used to determine metabolic indicators in fish, and disk electrophoresis in PAGE was used to determine the fractional composition of proteins.</p> <p><strong>Results</strong>. It was established that the steroid hormone 19-nortestosterone, the concentration of which in the water of the fish of the experimental groups was 50 and 200 μg/dm<sup>3</sup>, respectively, increased the level of total protein and albumin, the concentration of glucose, triglycerides, creatinine, inorganic phosphorus and iron in the hepatopancreas and did not affect the calcium content . In the blood plasma of carp, kept in water with a concentration of 19-nortestosterone of 50 μg/dm<sup>3</sup>, the content of proteins with a molecular weight of 240-231 increases; 215-179; 169-146 and 105-95 kDa, decreases - 35-33 kDa, fractions of proteins with a molecular weight of 228-220 appear; 116-105 and 48-44 kDa. An increase in the concentration of 19-nortestosterone in water to 200 μg/dm<sup>3</sup> increased the content of proteins with a molecular weight of 240-231 and 215-179 kDa in the blood plasma of fish, and decreased it by 35-33 kDa, causing the redistribution of individual protein fractions due to the appearance of proteins with a molecular weight of 240 -228; 116-105 and 48-44 kDa in the absence of proteins 42-41; 38-36 and 24-20 kDa.</p> <p><strong>Conclusions.</strong> Based on the obtained results, it was concluded that the anabolic synthetic steroid 19-nortestosterone stimulates the biosynthesis of proteins, the metabolism of carbohydrates and lipids, and its effect on the content of minerals in the hepatopancreas and the fractional composition of blood plasma proteins in carp fish was proven</p>2023-12-29T00:00:00+02:00Copyright (c) 2024 Mykola Zakharenko, Ella Romanovahttps://journals.uran.ua/sr_bio/article/view/295545Pathogenicity of non-pigmented and pigmented isolates of P. aeruginosa during long-term storage2023-12-30T13:07:23+02:00Yevheniia Vashchykyevgeniavashik1@gmail.comOlga Bobrytskaolga.bobritskaya2410@gmail.comSergiy Shtrygol'shtrygol@ukr.netAndriy Zakharievvagabond19973@gmail.comRuslan Dubindubinruslan1@gmail.comOlga Shapovalovashapolga2002@gmail.com<p><strong>The aim: </strong>to study the pathogenicity of non-pigmented P. aeruginosa isolates compared to those that synthesize pigment during long-term storage for broiler chickens.</p> <p><strong>Materials and methods.</strong> Bacteriological studies were carried out in accordance with generally accepted methods. Initially, P. aeruginosa and other bacterial microflora were isolated from poultry farms, pathological material from poultry. The type of microorganisms was identified using Bergey's identifier. The obtained isolates were examined by morphological characteristics (according to Gram), tincture, cultural, biochemical, pathogenic properties and sensitivity of selected cultures to antibiotics were studied. After the expiration of 3 years, the main biological properties of the isolates were monitored. To establish pathogenic properties, a bioassay was performed on white mice and one-week-old broiler chickens of the Cobb 500 cross (intraperitoneally by washout from daily agar culture at a dose of 0.2 cm<sup>3</sup>, which corresponds to the previously established LD50).</p> <p><strong>The results.</strong> With parenteral infection by washout from agar daily culture of pigmentless isolates at a dose of LD50, chickens died within the first - second day, in this case 87.50 % of the cultures showed pathogenicity. Experimental parenteral infection with washings from a daily culture of pigmented isolates of P. aeruginosa at a dose of LD50 led to the death of one-week-old chickens within 24-48 hours, in this case 75.00 % of the isolates showed pathogenicity.</p> <p>Clinical and pathological signs of infection with pigmented and non-pigmented P. aeruginosa isolates were similar.</p> <p><strong>Conclusions.</strong> A comparative analysis of cases of pathogenicity of non-pigmented and pigmented isolates of P. aeruginosa on one-week-old broiler chickens of the Cobb-500 cross was carried out. In the experiment, we found that among the non-pigmented isolates, compared to the pigmented pathogenic isolates, 12.5 % more were detected in chickens. This emphasizes the importance of differential diagnosis for pseudomonosis, because infection with non-pigmented strains often goes undiagnosed</p>2023-12-29T00:00:00+02:00Copyright (c) 2024 Yevheniia Vashchyk, Olga Bobrytska, Sergiy Shtrygol', Andriy Zakhariev, Ruslan Dubin, Olga Shapovalova