ScienceRise: Biological Science

Features of blood`s microcirculation at physical loads

Tetana Stanishevska — 2020-12-30

This research deals with the study of blood microcirculation peculiarities.

Materials and methods. 72 students of Bogdan Khmelnytsky Melitopol State Pedagogical University, aged 18–19, were examined. The experimental research consisted of the study of blood microcirculation functional state by means of Laser Doppler flowmetry (LDF) method. It helped to evaluate the state of tissue blood-circulation and to detect individual-typological peculiarities of blood microcirculation under the influence of physical activity (before and after exercise).

Results. Three types of blood microcirculation were identified by using LDF-metry. The normoemic type of blood microcirculation, characterized by the superposition of oscillatory rhythms and reflected the balance of the mechanisms of regulation of microcirculation. The hyperemic type, characterized by a «monotonous» LDF-gram with a high parameter of microcirculation, which reflects the relative predominance of metabolic mechanisms in the regulation of microcirculation. The hypoemic type, characterized by a «monotonous» LDF-gram with a low parameter of the microcirculation parameter, which reflects the decrease of vasomotor mechanisms in the regulation of microcirculation. According to the LDF-metric data, the examined students under intensive physical activity have a significant increase in microcirculatory status: by 6 % of the microcirculation parameter, by 28 % of the mean square deviation and by 45 % of the initial value of the coefficient of variation.

Conclusions. This dynamics of microcirculation shows that under the influence of physical exertion, a person creates significant functional reserves for the redistribution of blood flow and for more perfect intraorgan capillary blood flow. It was found, that in the process of physical activity, morpho-functional rearrangements of the human cardiovascular system occur. This reaction is formed by several components of blood microcirculation: blood flow in the transport direction, regulating blood supply in accordance with the needs of tissues and the exchange component of the histochemical barrier

Determination of the anticancer properties of cis- and trans-diadamanthylcarboxylates of dirhenium(III)

Natalia Shtemenko — 2020-12-30

The aim of the study. The aim of the work was to investigate in vivo anticancer activity of cis- and trans-diadamanthylcarboxylates of dirhenium(III) alone and together with cisplatin in form of nanobins.

Materials and methods. Model of tumor growth, Guerin’s carcinoma; intraperitoneal administration of cisplatin, dirhenium(III) compounds in liposomes and of binary liposomes, containing both cytostatics; volumes and final weights of tumors were measured.

Results. In vivo antitumor properties of two dirhenium(III) dicarboxylates with 1-adamantanecarboxylic acid moieties as ligands with cis- (I) and trans- (II) orientation of the carboxylic groups around a cluster fragment alone and together with cisplatin were presented; an attempt to understand differences in a possible mechanism of anticancer activity of the substances were undertaken. Antiradical and DNA-binding properties of I and II were the matter of consideration.

Conclusions. Cis- and trans- compounds of dirhenium I and II had close antitumor activity in vivo with a little bit superiority of the cis- analog. Mechanisms of anticancer activity of I and II are different and may also include monofunctional adduct formation and subsequent interstrand cross-linking for the II substance, formation of protein-DNA cross-links, etc.

Trends and outcomes of tuberculosis among cases at directly observed short course treatment (DOTS) at the Tepi public Health Center Southwest Ethiopia

Samuel Getachew — 2020-12-30

Background: Monitoring treatment outcomes and understanding the reasons for unsuccessful treatment have paramount importance for the tuberculosis control program. This study was designed to evaluate trends and treatment outcomes of tuberculosis patients at the Tepi Health Center and to identify the predictors of unsuccessful treatment outcome.

Method: Retrospective review of TB cases, registered in the Tepi health center from 2011-2018, was conducted using data, extracted from medical records of TB patients. The structured data extraction form was prepared and used to extract socio-demographic, clinical and outcome data of study cases. The case definition and the treatment outcome of patients were ascertained and reported in accordance with the World Health Organization guideline. A binary logistic regression model was fit to identify predictors of unsuccessful outcome.

Results: A total of 1651 TB patients, registered at the Tepi Public Health Center in between June 2011 and May 2018, were included in the study. Of all 924(56 %) were males and 1053 (63.8 %) cases were in between the age range of 15 and 35 years. HIV-status of 1019 TB cases was unknown and 189(11.4 %) of participants were HIV-positive. 457 (27.7 %) cases were diagnosed with extra pulmonary TB (EPTB) and 1194(72.3 %) were pulmonary TB patients, out of which 376(73.6 %) were smear-positive pulmonary TB (PTB+). The overall treatment success rate (TSR) of patients was 80.4 % (1327/1651), while it was 84.8 % (134/158), 80.2 % (410/511), and 78.3 % (148/189) among the transfer-in, PTB+, and HIV+ cases, respectively. Higher numbers of successful treatment outcomes were recorded among new (82.7 %) EPTB cases (84.7 %). The cure rate was 73.6 %(376/511) and 18(34/189) among patients with PTB+ and HIV+, respectively. Multiple logistic regression analysis indicated that residence sites (OR .763(.584, .996) and TB/HIV co-infection (OR 0.661(0.444, 0.985), were significantly associated with the treatment outcome. Rural residence was 27.1 % less likely to have successful treatment. There was significant heterogeneity in the odds of having successful treatment outcomes across years of initiating treatment.

Conclusion: The treatment success rate among study cases was lower than the WHO’s target and further efforts like availability of TB clinics in nearby sites and reducing rate of HIV infection should be made to improve the rate of successful treatment outcome

Characteristics of visible autumn migrations of the Dunnock prunella modularis (Linnaeus, 1758) in National Park “Prypiat-Stokhid” in 2012-2017

Mykhailo Khymyn — 2020-12-30

The aim. The main purpose of the study was to find out the peculiarities of autumn migrations of birds, in particular such little-studied species as the Dunnock. This is necessary for the development and implementation of a management plan in the National Park for a whole complexes of migratory birds and separate species, first of all rare or small in number quantity.

Materials and methods. Our research was conducted at the Prypiat River near Svalovychi village in Liubeshiv district, Volyn region of Ukraine in September and October 2012–2017. Observations were performed visually, starting in the morning half an hour before sunrise and ending in the evening half an hour after sunset (all hours of the light part of a day, without interruption). The light part of a day was divided into hour of observations, taking into the daily changes of sunrise and sunset. Flight altitude was determined visually, and flight directions – by 8 rhumbs.

Results. There were 2124 individuals of the Dunnock counted during the study of visible autumn migration birds at one observation point in 2012–2017. The most bird migrated in flocks, but a significant part of them flew alone. The most numerous migrants this species were in small flocks – 2–5 individuals (89.9 % of all counted birds in flocks). There are from 2 to 38 individuals in one flock, on average – 3.2±0.15 individuals. The main passage lasted from the 2nd decade of September to the middle of the 2nd decade of October. The majority of them flew in the morning, in the first 3 hours of observations (75.5–83.5 % of all counted birds in different years, in average 79.6 %). The majority birds of this species were observed in flight within altitudes between 30 and 40 m (59.4 %) and the predominant direction of flight was W (52.4 %) and some less – SW (45.4 %).

Conclusions. According to the results of 6-years research, the peculiarities of visible autumn migrations of the Dunnock have been clarified (the main passage – the 2nd decade of September – middle of the 2nd decade of October, majority of birds flew in the first 3 hours of observations, used altitudes between 30 and 40 m and the W and SW directions of passage). The results of our research are the basis for the further study of this species, planning and implementation of conservation measures for birds in the National Park “Prypiat-Stokhid”. The obtained data are also a supplement to the state of study of this species in Ukraine