Hematological indicators of rats for artificial hypobiosis

Authors

  • Anna Umanska National University of Life and Environmental Sciences of Ukraine Heroiv Oborony str., 15, Kyiv, Ukraine, 03041, Ukraine https://orcid.org/0000-0001-7335-0851
  • Dmytro Melnichuk Public Organization "The Council of Heroes of Ukraine in the agroindustrial complex" Solomianska str., 2a, Kyiv, Ukraine, 02000, Ukraine https://orcid.org/0000-0002-9013-4170
  • Lilia Kalachnyuk National University of Life and Environmental Sciences of Ukraine Heroiv Oborony str., 15, Kyiv, Ukraine, 03041, Ukraine

DOI:

https://doi.org/10.15587/2519-8025.2017.118786

Keywords:

hypobiosis, hypercapnia, hypoxia, erythrocyte, lymphocyte, platelet, blood, rats, hematology, leucocyte

Abstract

The use of hypobiotic state in the medical practice is rather urgent in the modern world. Three most important conditions of creation of artificial hypobiosis are: hypoxia, hypothermia and hypercapnia. The most important parameters that characterize homeostasis of the organism at artificially created conditions of hypobiosis are hematological indices. White non-pedigreed male rats with the mass 180–200 g, kept in standard vivarium conditions, were used for the experiment. Animals were divided in groups: control (intact animals) and experimental ones – the first group (artificial hypobiosis), the second group in 24 hours after going out from artificial hypobiosis. The number of animals in each group n=5. Experiments were realized according to requirements of the “European convention about protection of vertebral animals, used for experimental and other scientific needs” (Strasburg, France, 1985), general ethic principles of experiments on animals, accepted by the First national congress of Ukraine on bioethics (2001). As a result of realized studies there was demonstrated the essential increase of erythrocytes and platelets together with the decrease of the leycocytes content. Under conditions of hypoxia and hypercapnia the growth of the erythrocytes level can be explained by the increase of the carbonic acid level, and the platelets level grows because of the decrease of blood fluidity at artificial hypobiosis

Author Biographies

Anna Umanska, National University of Life and Environmental Sciences of Ukraine Heroiv Oborony str., 15, Kyiv, Ukraine, 03041

Postgraduate student

Academician M. F. Guliy Department of Biochemistry 

Dmytro Melnichuk, Public Organization "The Council of Heroes of Ukraine in the agroindustrial complex" Solomianska str., 2a, Kyiv, Ukraine, 02000

Doctor of Biological Sciences, Professor, Academician of NAS and NAAS of Ukraine, Head of Public Organization

Lilia Kalachnyuk, National University of Life and Environmental Sciences of Ukraine Heroiv Oborony str., 15, Kyiv, Ukraine, 03041

Doctor of Biological Sciences, Professor, Academician of Academy of Sciences of Higher Education of Ukraine, Laureate of State Prize of Ukraine in the Field of Science and Technology

Academician M. F. Guliy Department of Biochemistry 

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Published

2017-12-31

How to Cite

Umanska, A., Melnichuk, D., & Kalachnyuk, L. (2017). Hematological indicators of rats for artificial hypobiosis. ScienceRise: Biological Science, (6 (9), 7–9. https://doi.org/10.15587/2519-8025.2017.118786

Issue

No. 6 (9) (2017)

Section

Biological Sciences

License

Copyright (c) 2017 Anna Umanska, Dmytro Melnichuk, Lilia Kalachnyuk

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