Changes of macro- and microelement composition of the liver in rats for artificial hypobiosis

Authors

DOI:

https://doi.org/10.15587/2519-8025.2018.124869

Keywords:

hypobiosis, hypothermia, hypercapnia, hypoxia, heart, macroelements, microelements, ferrum, potassium, sodium

Abstract

Surgeons throughout the world face numerous urgent questions as to alternative ways of anesthesia that don’t need to switch on apparatuses: “artificial heart”, “artificial lungs ventilation” at short-term surgical interventions. One of such ways can be just artificial hypobiosis. The main conditions for transferring to this stage are: hypoxia, hypercapnia, hypothermia. For using this method in surgical practice at short-term surgical interventions successfully in future, it is necessary to study a series of urgent questions, connected with its safety more detail. White usual male-rats with the mass 180–200 g, kept in standard vivarium conditions, were used in the study. The animals were divided in groups: control (intact animals) and experimental artificial hypobiosis state. The number of animals in each group n = 8. The experiments were conducted, according to requirements of the “European convention about protection of vertebral animals, used with experimental and other scientific purposes” (Strasburg, France, 1985), according to general ethic principles of experiments on animals, accepted by the First national congress of bioethics of Ukraine (2001). As a result of the conducted studies there was demonstrated the essential increase of Potassium, Sodium and Ferrum, at the same time the Calcium content decrease. The growth of Potassium and Sodium in the organism can be explained by the delay of liquid in the organism, because flowability of liquids in the organism decreases at artificially-created hypobiotic conditions

Author Biographies

Anna Umanska, National University of Life and Environmental Sciences of Ukraine Heroiv Oborony str., 15, Kyiv, Ukraine, 03041

Postgraduate student

Academician M. F. Guliy Department of Biochemistry 

Dmytro Melnichuk, Public Organization "The Council of Heroes of Ukraine in the agroindustrial complex" Solomianska str., 2a, Kyiv, Ukraine, 02000

Doctor of Biological Sciences, Professor, Academician of NAS and NAAS of Ukraine, Head of Public Organization

Lilia Kalachnyuk, National University of Life and Environmental Sciences of Ukraine Heroiv Oborony str., 15, Kyiv, Ukraine, 03041

Doctor of Biological Sciences, Professor, Academician of Academy of Sciences of Higher Education of Ukraine, Laureate of State Prize of Ukraine in the Field of Science and Technology

Academician M. F. Guliy Department of Biochemistry

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Published

2018-03-01

How to Cite

Umanska, A., Melnichuk, D., & Kalachnyuk, L. (2018). Changes of macro- and microelement composition of the liver in rats for artificial hypobiosis. ScienceRise: Biological Science, (1 (10), 45–47. https://doi.org/10.15587/2519-8025.2018.124869

Issue

Section

Biological Sciences