The study of oxidative stress indicators in rats with a simulated acute hepatitis and correction with a thick extract from reishi mushrooms

Authors

DOI:

https://doi.org/10.15587/2519-8025.2021.239920

Keywords:

reishi mushrooms, paracetamol, hepatitis, dry extract, oxidative stress, antioxidant properties

Abstract

The aim. To study the effect of a dry extract from reishi mushrooms on the activity of lipoperoxidation and oxidative modification of proteins under the conditions of a simulated paracetamol hepatitis in rats.

Materials and methods. The study was performed on white male rats. The animals were divided into 10 groups, each included 6 animals. Acute hepatitis was simulated by the intragastric administration of paracetamol in the dose of 1250 mg/kg once per day (for 2 days). Correction of the pathology induced was performed with a dry extract of reishi mushrooms in the dose of 100 mg/kg of the body weight. The reference drug “Silybor” was administered in the dose of 20 mg/kg of the animal body weight. On Day 3, 7 and 10 from the beginning of the lesion, rats were euthanized using sodium barbamyl. The liver homogenate and blood serum were used for the studies. The activity of free radical oxidation processes under the conditions of acute toxic hepatitis and after the introduction of corrective factors was assessed by superoxide dismutase, catalase activity, the content of TBA-AP and OMP products.

Results and discussion. The development of acute paracetamol hepatitis in rats and damage of hepatocyte membranes are indicated by an increase in the content of TBA-active products, products of oxidative modification of neutral and basic proteins in the serum and liver of animals. Simultaneously, a decrease in the activity of catalase and superoxide dismutase was observed. After correction of the pathology induced with a dry extract of reishi mushrooms a significant increase in the activity of antioxidant enzymes, a decrease in the content of lipid peroxidation products and oxidative modification of proteins in the serum and liver of the affected animals were observed.

Conclusions. It has been experimentally proven that the use of dry extract of Reishi mushrooms in paracetamol hepatitis in rats caused a significant decrease in catalase and superoxide dismutase activity, a decrease in TBA-AP, neutral and basic 2,4-DNPH in the serum and liver of animals. The results of the research indicate an effective impact of reishi mushrooms dry extract on the normalization of lipoperoxidation, oxidative modification of proteins and antioxidant protection

Author Biographies

Iryna Herasymets, I. Ya. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmacology with Clinical Pharmacology

Liudmila Fira, I. Ya. Horbachevsky Ternopil National Medical University

Doctor of Biological Sciences, Professor, Head of Department

Department of Pharmacy

Igor Medvid, I. Ya. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Microbiology, Virology and Immunology

References

  1. Herasymets, I. I., Fira, L. S., Medvid, I. I. (2019). Parameters of the experimental extract of reishi mushrooms acute toxicity determination. Fitoterapia, 3 (3), 46–49. doi: http://doi.org/10.33617/2522-9680-2019-3-46
  2. Kyslychenko, V. S., Harnyk, T. P., Zhuravel, I. O. (2013). Hryby yak perspektyvna syrovyna dlia zastosuvannia v medytsyni. Fitoterapiia. Chasopys, 1, 31–35.
  3. Veljović, S., Veljović, M., Nikićević, N., Despotović, S., Radulović, S., Nikšić, M., Filipović, L. (2017). Chemical composition, antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts. Journal of Food Science and Technology, 54 (5), 1312–1320. doi: http://doi.org/10.1007/s13197-017-2559-y
  4. Liang, Z., Yi, Y., Guo, Y., Wang, R., Hu, Q., Xiong, X. (2014). Chemical Characterization and Antitumor Activities of Polysaccharide Extracted from Ganoderma lucidum. International Journal of Molecular Sciences, 15 (5), 9103–9116. doi: http://doi.org/10.3390/ijms15059103
  5. Herasymets, I., Fira, L., Medvid, I. (2019). Study of the Reishi mushrooms dry extract hepatoprotective properties. Ukrainian Biopharmaceutical Journal, 4 (61), 28–34. doi: http://doi.org/10.24959/ubphj.19.232
  6. Vetchinkina, Ye., Shyrokov, A. A., Bucharskaia, A. B. (2015). Vyyavlenie protivoopukholevoy aktivnosti gribnogo mitseliya i plodovikh tel. Bazidialnykh gribov. Uspekhi meditsinskoy mikolohii, 14 (14), 462–471.
  7. Zeng, P., Guo, Z., Zeng, X., Hao, C., Zhang, Y., Zhang, M. et. al. (2018). Chemical, biochemical, preclinical and clinical studies of Ganoderma lucidum polysaccharide as an approved drug for treating myopathy and other diseases in China. Journal of Cellular and Molecular Medicine, 22 (7), 3278–3297. doi: http://doi.org/10.1111/jcmm.13613
  8. Wang, J., Cao, B., Zhao, H., & Feng, J. (2017). Emerging Roles of Ganoderma Lucidum in Anti-Aging. Aging and Disease, 8 (6), 691–707. doi: http://doi.org/10.14336/ad.2017.0410
  9. Wu, D.-T., Deng, Y., Chen, L.-X., Zhao, J., Bzhelyansky, A., Li, S.-P. (2017). Evaluation on quality consistency of Ganoderma lucidum dietary supplements collected in the United States. Scientific Reports, 7 (1). doi: http://doi.org/10.1038/s41598-017-06336-3
  10. Kalko, K. O., Drohovoz, S. M. (2017). Circadian dependence of Antral hepatoprotective activity on the model of acute paracetamol-induced hepatitis in rats. Farmakolohiia ta likarskatoksykolohiia, 2 (54), 62–68.
  11. Zmitrovich, I. V. (2014). Griby posylayut signaly. Planetahribov, 2 (6), 21–27.
  12. Gross, D., Tolba, R. H. (2015). Ethics in Animal-Based Research. European Surgical Research, 55 (1-2), 43–57. doi: http://doi.org/10.1159/000377721
  13. Lushchak, V. I., Bahniukova, T. V., Lushchak, O. V. (2004). Pokaznyky oksydatyvnoho stresu. Tiobarbituraktyvni produkty i karbonilni hrupy bilkiv. Ukrainskyibiokhimichnyi zhurnal, 26, 136–141.
  14. Dubinina, Ye. Ye. (2001). Okislitelniy stress i okislitelnaya modifikatsiya belkov. Med. Chemistry, 2, 5–12.
  15. Dubinina, Ye. Ye. (2001). Rol aktivnykh form kisloroda v kachestve signalnykh molekul v metabolizme tkaney pri sostoyanii okislitelnogo stressa. Voprosy meditsinskoi khimii, 47 (6), 561–581.
  16. Babizhayev, M. A. (2011). Mitochondria induce oxidative stress, generation of reactive oxygen species and redox state unbalance of the eye lens leading to human cataract formation: disruption of redox lens organization by phospholipid hydroperoxides as a common basis for cataract. Cell Biochemistry and Function, 29 (3), 183–206. doi: http://doi.org/10.1002/cbf.1737
  17. Koroliuk, M. A., Ivanova, L. I., Maiorova, I. H. (1988). Metod opredeleniya aktivnosti katalazy. Laboratornoe delo, 1, 16–19.
  18. Shelamova, M. A., Insarova, N. I., Lieshchienko, V. H. (2010). Statisticheskii analiz miediko-biologichieskikh dannykh s ispolzovaniiem programmy EXCEL. Minsk: BGMU, 96.
  19. Jannot, A.-S., Agoritsas, T., Gayet-Ageron, A., Perneger, T. V. (2013). Citation bias favoring statistically significant studies was present in medical research. Journal of Clinical Epidemiology, 66 (3), 296–301. doi: http://doi.org/10.1016/j.jclinepi.2012.09.015
  20. Lavryshyn, Y. Y., Varkholyak, I. S., Martyschuk, T. V., Guta, Z. A., Ivankiv, L. B., Paladischuk, O. R. et. al. (2016). The biological significance of the antioxidant defense system of animal's body. Scientific Messenger LNUVMBT named after S.Z. Gzhytskyj, 18 (2 (66)), 100–111.
  21. Diorditsa, Ya. (2019). Antioxidant system activity in liver of ratsunder conditions of acute hepatitis during correctionwith antioxidant complexes. Visnyk of the Lviv University. Series Biology, 81, 12–20.
  22. Drogovoz, S. M., Mishchenko, O. M., Kalko, K. O., Bogdan, N. S., Gerush, O. V. (2019). Circadian developmental rhythms of the experimental paracetamol hepatitis and the effect of hepatoprotectors on the activity of pro-oxidative / antioxidant and cytolytic processes. Clinical pharmacy, 23 (2), 15–24. doi: http://doi.org/10.24959/cphj.19.1485
  23. Ryeznikov, O. H., Polumbryk, O. M., Baljon, Ya. H. (2014). Pro- and antioxidant systems and pathological processes in humans. Visn. NAN Ukrainy, 10, 17–29.
  24. Kravchenko, V. M., Shovkova, Z. V., Senyuk, I. V., Shovkova, O. V. (2020). The study of lipotropic action of extract from fruit prunus domestica «Prunofit» byanimal models of alcoholic liver diseases. Farmatsevtychnyi Zhurnal, 6, 86–91. doi: http://doi.org/10.32352/0367-3057.6.20.09

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Published

2021-09-30

How to Cite

Herasymets, I., Fira, L., & Medvid, I. (2021). The study of oxidative stress indicators in rats with a simulated acute hepatitis and correction with a thick extract from reishi mushrooms. ScienceRise: Biological Science, (3 (28), 4–9. https://doi.org/10.15587/2519-8025.2021.239920

Issue

No. 3 (28) (2021)

Section

Biological research

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Copyright (c) 2021 Iryna Herasymets, Liudmila Fira, Igor Medvid

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