Comparatyive determination of proteins, nitrogen-containing compounds and lipids content in blood plasma and ascitic fluid in patients with liver cirrhosis

Authors

DOI:

https://doi.org/10.15587/2519-4798.2017.109164

Keywords:

liver cirrhosis, ascitic fluid, blood plasma, pathogenetically substantiated treatment

Abstract

The aim of the work is the determination of proteins concentration in blood plasma and ascetic fluid and also toxic and atherogenic properties of ascitic fluid in patients with different stages of liver cirrhosis for the further choice of the individual treating tactics.

Research methods: 262 patients with liver cirrhosis, according to the diagnostic examination, who demonstrated the different severity of the pathological process in the liver parenchyma, were divided in 4 groups. In blood plasma and ascitic fluid of patients with liver cirrhosis were determined the concentration of protein, general content of bilirubin, concentration of final nitrogen, creatinine, urea, cholesterol and lecithin. 

Results: the obtained results demonstrate the disorder of the protein homeostasis and increase of the content of atherogenic lipoproteins, as a result of which hepatocyte membrane structures in patients with liver cirrhosis are destructed.  The content of studied components in ascitic fluid was identical (and sometimes even more) to the one in blood plasma – it allows to consider ascitic fluid as a plasma-replacing component in patients with liver cirrhosis, especially at the hepatic failure progression.

The degree of the disorder of the functional activity of the liver parenchyma was studied. The author of the article concludes that at choosing the surgical tactics in patients with liver cirrhosis, complicated with acsites, the degree of homeostasis disorders expressiveness must be taken into account

Author Biography

Alexander Dzygal, Odessa National Medical University Valikhovsky lane, 2, Odessa, Ukraine, 65082

PhD, Associate Professor

Department of Surgery Nо. 3 with the course of neurosurgery

References

  1. Harris, R., Harman, D. J., Card, T. R., Aithal, G. P., Guha, I. N. (2017). Prevalence of clinically significant liver disease within the general population, as defined by non-invasive markers of liver fibrosis: a systematic review. The Lancet Gastroenterology & Hepatology, 2 (4), 288–297. doi: 10.1016/s2468-1253(16)30205-9
  2. Garbuzenko, D. V. (2016). Current approaches to the management of patients with liver cirrhosis who have acute esophageal variceal bleeding. Current Medical Research and Opinion, 32 (3), 467–475. doi: 10.1185/03007995.2015.1124846
  3. Andreev, G. N., Borisov, A. E., Ibadil'din, A. S. et. al. (1999). Patogenez, diagnostika i lechenie cirrozov pecheni, oslozhnennyh rezistentnym ascitom. Nizhniy Novgorod: Izd-vo Nizhegorod. gos. akad., 191.
  4. Ascione, T., Di Flumeri, G., Boccia, G., De Caro, F. (2017). Infections in patients affected by liver cirrhosis: an update. Infez Med., 25 (2), 91–97.
  5. Abdukadyrova, M. A. (1996). Virus gepatita S – odin iz glavnyh ekologicheskih faktorov hronicheskih gepatitov. Hronicheskie zabolevaniya pecheni ot virusnogo gepatita do cirroza pecheni. Tashkent, 4–5.
  6. Ibadil'din, A. S., Andreev, G. N., Borisov, A. E. (1999). Polisindromnost' cirroza pecheni. Nizhniy Novgorod: Izd-vo Nizhegorod. gos. akad., 196.
  7. Frolov, V. M., Petrunya, A. M., Pinskiy, L. L. (1996). Sostoyanie mikrogemodinamiki i immunnyy status u bol'nyh s hronicheskimi virusnymi porazheniyami pecheni i ih korrekciya. Vrachebnoe delo, 10-12, 144–146.
  8. Chesnokov, E. V., Kashuba, E. A. (2000). Hronicheskie gepatity i cirrozy pecheni virusnoy etiologii. Tyumen', 286.
  9. Reddy, S. S., Civan, J. M. (2016). From Child-Pugh to Model for End-Stage Liver Disease. Medical Clinics of North America, 100 (3), 449–464. doi: 10.1016/j.mcna.2015.12.002
  10. Ivashkin, V. T. (Ed.) (2008). Klinicheskie rekomendacii. Gastroenterologiya. Moscow: GEOTAR-Media, 208.
  11. Kerefova, Z. Sh., Pshegusova, M. H., Gubzhokova, O. Z. (2015). Mediko-social'nye aspekty cirrozov pecheni virusnoy etiologii. Simvol nauki, 11, 187–190.
  12. Yu, X., Guo, R., Ming, D., Su, M., Lin, C., Deng, Y. et. al. (2014). Ratios of regulatory T cells/T-helper 17 cells and transforming growth factor-β1/interleukin-17 to be associated with the development of hepatitis B virus-associated liver cirrhosis. Journal of Gastroenterology and Hepatology, 29 (5), 1065–1072. doi: 10.1111/jgh.12459
  13. Alekseeva, I. N., Bryzgina, T. M., Pavlovich, S. I., Il'chevich, A. B. (1991). Pechen' i immunologicheskaya reaktivnost'. Kyiv, 150.
  14. Luk'yanov, S. V. (2005). Reinfuziya asciticheskoy zhidkosti pri hirurgicheskom lechenii sindroma portal'noy gipertenzii. Rostov: Rostovskiy gosudarstvennyy medicinskiy universitet, 20.
  15. Sapronova, N. G., Luk'yanov, S. V., Chigaeva, E. V. (2013). Osobennosti lecheniya pacientov s virus-associirovannym cirrozom pecheni. Sovremennye problemy nauki i obrazovaniya, 6. Available at: http://www.science-education.ru/ru/article/view?id=10856
  16. Yarema, I. V., Omarov, I. M. (2000). Hirurgicheskaya korrekciya gemo- i limfoobrashcheniya pri cirroze pecheni. Vestnik hirurgii, 159 (6), 12–14.
  17. Ginès, P., Cárdenas, A., Arroyo, V., Rodés, J. (2004). Management of Cirrhosis and Ascites. New England Journal of Medicine, 350 (16), 1646–1654. doi: 10.1056/nejmra035021
  18. Rochling, F. A., Zetterman, R. K. (2009). Management of Ascites. Drugs, 69 (13), 1739–1760. doi: 10.2165/11316390-000000000-00000
  19. Cadman, E., Bostwick, J. R., Eichberg, J. (1979). Determination of protein by a modified Lowry procedure in the presence of some commonly used detergents. Analytical Biochemistry, 96 (1), 21–23. doi: 10.1016/0003-2697(79)90548-7
  20. Noma, A., Okabe, H., Netsu-Nakayama, K., Ueno, Y., Shinohara, H. (1979). Improved method for simultaneous determination of cholesterol in high- and low-density lipoproteins. Clin. Chem., 25 (8), 1480–1481.
  21. Kucherenko, N. E., Vasil'ev, A. N. (1985). Lipidy. Kyiv: Vishcha shkola, 247.
  22. Scheiner, B., Lindner, G., Reiberger, T., Schneeweiss, B., Trauner, M., Zauner, C., Funk, G.-C. (2017). Acid-base disorders in liver disease. Journal of Hepatology. doi: 10.1016/j.jhep.2017.06.023
  23. Mogilevec, E. V. (2014). Hirurgicheskie aspekty korrekcii asciticheskogo sindroma u pacientov s cirrozom pecheni i portal'noy gipertenziey. Zhurnal Grodnenskogo gosudarstvennogo medicinskogo universiteta, 3, 5–11.
  24. Carrier, P., Jacques, J., Debette-Gratien, M., Legros, R., Sarabi, M., Vidal, E. et. al. (2014). L’ascite non liée à la cirrhose : physiopathologie, diagnostic et étiologies. La Revue de Médecine Interne, 35 (6), 365–371. doi: 10.1016/j.revmed.2013.12.001

Published

2017-08-31

How to Cite

Dzygal, A. (2017). Comparatyive determination of proteins, nitrogen-containing compounds and lipids content in blood plasma and ascitic fluid in patients with liver cirrhosis. ScienceRise: Medical Science, (8 (16), 15–19. https://doi.org/10.15587/2519-4798.2017.109164

Issue

Section

Medical Science