Research of the toxicological profile of the new gel, which contains an extract of the oak bark and aloe extract

Authors

DOI:

https://doi.org/10.15587/2519-4852.2018.123978

Keywords:

acute toxicity, extract of oak bark, aloe extract, gel, toxicity, extract

Abstract

On these days periodontal diseases are widely distributed and diagnosed in more than 75 % of the world population and occurs in all age groups. Drugs based on medicinal plants should be considered as promising products, which have therapeutic effect with minimum adverse reactions.

The aim of this work was to study the acute toxicity of new gel containing plant extract of oak bark and extract of aloe.

Methods. Acute toxicity was studied in accordance with the guidelines on white nonlinear rats with 180–220 g in weight, by several steps: the study of acute toxicity of components of a new gel, the study of acute toxicity of a new gel under intragastric administration, intraperitoneal and skin administration.

Results. It was established that it is not possible to determine LD50 by intragastric administration of extract of oak bark and extract of aloe, since administration of a maximum dose of 15100 mg / kg did not cause lethality of rats. In order to confirm this assertion, in further studies the acute toxicity of extracts was studied only at a dose of 15100 mg / kg. In terms of intraperitoneally administration, LD50 of extract of oak bark is 2580 (1930–3220) mg / kg, LD50 of extract of aloe – 2180 (1460–2900) mg / kg. At the subsequent stages of the study during the observation for 14 days, there were no visible signs of the toxic effect of the new gel on the functional state of the animals at the maximum recommended doses: intragastric – 15100 mg / kg and skin application – 22600 mg / kg.

Conclusions. The complex of the conducted studies allowed establishing that the new gel does not have a toxic effect on organs and systems of experimental animals and does not have a lethal action. Extract of oak bark and extract of aloe under conditions of intragastric administration are classified as VI class of toxicity "Relatively harmless substances". In case of intraperitoneally administration, the studied extracts were classified as V class of toxicity "Practically nontoxic substances". According to the study of acute toxicity, the new gel, in conditions of intragastric administration and skin application, belongs to the VI class of toxicity "Relatively harmless substances"

Author Biographies

Natalya Tsubanova, Institute of Pharmacy Professionals Qualification Improvement of National University of Pharmacy Zakhysnykiv Ukrainy sq., 17, Kharkiv, Ukraine, 61001

Doctor of Pharmaceutical Sciences, Professor

Department of General Pharmacy and Safety of Drugs

Dmytro Zhurenko, Institute of Pharmacy Professionals Qualification Improvement of National University of Pharmacy Zakhysnykiv Ukrainy sq., 17, Kharkiv, Ukraine, 61001

Postgraduate Student

Department of General Pharmacy and Safety of Drugs 

Tatiana Sakharova, National University of Pharmacy Pushkinska str., 53, Kharkiv, Ukraine, 61002

Doctor of Pharmaceutical Sciences, Professor

Department of Clinical Pharmacology and Clinical Pharmacy

References

  1. Borisenko, A. V. (2013). Influence of periodontal diseases on the general state of the organism. Health of the Society, 1, 32–37.
  2. Savelyeva, N. M. (2017). Features of the clinic, diagnostics, treatment and prophylaxis of generalized periodontitis in patients with parasitic invasion. Odessa, 398.
  3. Panezai, J., Ghaffar, A., Altamash, M., Sundqvist, K.-G., Engstrom, P.-E., Larsson, A. (2017). Correlation of serum cytokines, chemokines, growth factors and enzymes with periodontal disease parameters. PLOS ONE, 12 (11), 188–194. doi: 10.1371/journal.pone.0188945
  4. Wang, Y., Andrukhov, O., Rausch-Fan, X. (2017). Oxidative Stress and Antioxidant System in Periodontitis. Frontiers in Physiology, 8. doi: 10.3389/fphys.2017.00910
  5. Stanko, P., Izakovicova Holla, L. (2014). Bidirectional association between diabetes mellitus and inflammatory periodontal disease. A review. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 158 (1), 35–38. doi: 10.5507/bp.2014.005
  6. Aoyama, N., Suzuki, J.-I., Kobayashi, N., Hanatani, T., Ashigaki, N., Yoshida, A. et al. (2017). Associations among tooth loss, systemic inflammation and antibody titers to periodontal pathogens in Japanese patients with cardiovascular disease. Journal of Periodontal Research, 53 (1), 117–122. doi: 10.1111/jre.12494
  7. Hamilton, J. A., Hasturk, H., Kantarci, A., Serhan, C. N., Van Dyke, T. (2017). Atherosclerosis, Periodontal Disease, and Treatment with Resolvins. Current Atherosclerosis Reports, 19 (12), 57–65. doi: 10.1007/s11883-017-0696-4
  8. Pritchard, A. B., Crean, S., Olsen, I., Singhrao, S. K. (2017). Periodontitis, Microbiomes and their Role in Alzheimer’s Disease. Frontiers in Aging Neuroscience, 9, 336–338. doi: 10.3389/fnagi.2017.00336
  9. Ramesh, A., Varghese, S., Doraiswamy, J., Malaiappan, S. (2016). Herbs as an antioxidant arsenal for periodontal diseases. Journal of Intercultural Ethnopharmacology, 5 (1), 92–96. doi: 10.5455/jice.20160122065556
  10. Tsubanova, N. A., Hohlenkova, N. V., Zhurenko, D. S., Matthew, І. І. (2016). Pat. No. 109792 UA. Pharmaceutical composition in the form of a dental gel with parodontoprotective action. MPK A61K 36/49, A61K 36/889, A61K 129/00, A61P 1/02. No. U 2016 01693; declareted: 23.02.2016; published: 12.09.2016, Bul. No. 17, 5.
  11. Zhurenko, D. S., Sagittarius, O. P., Hochlenkova, N. V., Matthew, I. I. (2016). Study of antimicrobial activity of «Аloedental» gel for treatment of periodontal diseases. Medicines of Ukraine plus, 3 (28), 34–36.
  12. Tsubanova, N. A., Hohlenkova, N. V., Zhurenko, D. S., Matthew, I. I. (2016). Research of efficiency new gel containing an extract of oak bark and extract of aloe vera under condition protamine periodontitis. Ukrainian biopharmaceutical journal, 2 (43), 27–31.
  13. Yarborough, M., Dirnagl, U. (2017). Preclinical research: Meet patients to sharpen up research. Nature, 551 (7680), 300–310. doi: 10.1038/d41586-017-06024-2
  14. Boncler, M., Golanski, J., Lukasiak, M., Redzynia, M., Dastych, J., Watala, C. (2017). A new approach for the assessment of the toxicity of polyphenol-rich compounds with the use of high content screening analysis. PLOS ONE, 12 (6), 180–188. doi: 10.1371/journal.pone.0180022
  15. Stefanova, O. V. (Ed.) (2001). Preclinical research of medicinal products. Kyiv: Avicenna, 528.
  16. Reznikov, O. G. (2003). General ethical principles of experiments on animals. Endocrinology, 8 (1), 142–145.
  17. Sidorov, K. K. (1973). On the classification of toxicity of poisons in parenteral methods of administration. Toxicology of new industrial chemicals. Moscow, 13, 47–57.

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Published

2018-02-21

How to Cite

Tsubanova, N., Zhurenko, D., & Sakharova, T. (2018). Research of the toxicological profile of the new gel, which contains an extract of the oak bark and aloe extract. ScienceRise: Pharmaceutical Science, (1 (11), 21–25. https://doi.org/10.15587/2519-4852.2018.123978

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Section

Pharmaceutical Science