Development of the spectrophotometric method for the determination of metoprolol in tablets by using bromophenol blue

Authors

DOI:

https://doi.org/10.15587/2519-4852.2022.270311

Keywords:

bromophenol blue, metoprolol tartrate, spectrophotometry, validation, pharmaceutical analysis

Abstract

The aim of the work was to develop a simple, economical and eco-friendly spectrophotometric method for determining metoprolol tartrate in tablets based on the reaction with bromophenol blue (BPB).

Material and methods: A double–beam Shimadzu UV-Visible spectrophotometer, with a spectral bandwidth of 1 nm wavelength accuracy ±0.5 nm, Model –UV 1800 (Japan), Software UV-Probe 2.62, and a pair of 1 cm matched quartz cells, was used to measure the absorbance of the resulting solution. All the chemicals were used in analytical reagent grade. Pharmacopeial standard samples of metoprolol tartrate and bromophenol blue (BPB) were provided by Sigma-Aldrich (≥ 98%, HPLC). The used dosage forms of metoprolol tartrate are tablets of Metoprolol 50 mg and 100 mg.

Results and discussion: The method of spectrophotometric determination of the quantitative content of metoprolol tartrate based on its reaction with BPB in a methanol solution has been developed. The stoichiometric ratios of the reactive components as 1:1 were obtained by the methods of continuous changes and the saturation method. The developed method of quantitative determination of metoprolol tartrate was validated. The linearity regression equation was y = 0.0373x + 0.0038, and the obtained correlation coefficient was R2=0.9984. A linear relationship was found between absorbance at λmax and concentration of metoprolol tartrate in the range of 9.56-15.02 µg/mL. The LOD and LOQ values were calculated to be 0.81 µg/mL and 2.67 µg/mL.

Conclusions. A simple, economical and eco-friendly spectrophotometric method has been developed for the quantitative determination of metoprolol tartrate in tablets based on the reaction with BPB. The developed method of quantitative determination of metoprolol tartrate was validated in accordance with the requirements of SPhU. We suggest our work with offered detailed and successful solutions for the mentioned aim with less sophisticated equipment for QC lab for routine manufacturing control

Supporting Agency

  • Ministry of Health of Ukraine under project number 0120U104201

Author Biographies

Mariana Horyn, I. Horbachevsky Ternopil National Medical University

Assistant

Department of Pharmaceutical Chemistry

Liubomyr Kryskiw, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Tetyana Kucher, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Olha Poliak, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Nadiya Zarivna, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Hryhorii Zahrychuk, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor, Head of Department

Department of General Chemistry

Dmytro Korobko, I. Horbachevsky Ternopil National Medical University

PhD, Associate Professor

Department of Pharmaceutical Chemistry

Kateryna Peleshok, I. Horbachevsky Ternopil National Medical University

PhD, Assistant

Department of Pharmaceutical Chemistry

Liliya Logoyda, I. Horbachevsky Ternopil National Medical University

Doctor of Pharmaceutical Sciences, Professor, Head of Department

Department of Pharmaceutical Chemistry

References

  1. Tucker, W. D., Sankar, P., Kariyanna, P. Th. (2022). Selective Beta-1-Blockers. Available at: https://www.ncbi.nlm.nih.gov/books/NBK499982/#:~:text=The%20cardio%2Dselective%20beta%2D1,acebutolol%2C%20metoprolol%2C%20and%20nebivolol
  2. Metoprolol tartrate. Available at: https://go.drugbank.com/salts/DBSALT000862
  3. uropean Pharmacopoeia (2020). Available at: https:www.edqm.eu/en/european-pharmacopoeia-ph-eur-10th-edition Last accessed: 04.07.2022
  4. Cesme, M., Tarinc, D., Golcu, A. (2011). Spectrophotometric Determination of Metoprolol Tartrate in Pharmaceutical Dosage Forms on Complex Formation with Cu(II). Pharmaceuticals, 4 (7), 964–975. doi: https://doi.org/10.3390/ph4070964
  5. Nabil, A. F., Eman, M. S. (2015). Spectrophotometric determination of metoprolol in pharmaceutical formulation by charge transfer complexation. International Journal of Chemical Studies, 3, 24–29. Available at: https://www.academia.edu/16235931/Spectrophotometric_determination_of_metoprolol_in_pharmaceutical_formulation_by_charge_transfer_complexation
  6. Donchenko, A., Vasyuk, S. (2018). Spectrophotometric determination of metoprolol tartrate in pure and dosage forms. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 42 (1), 33–42. doi: https://doi.org/10.1501/eczfak_0000000600
  7. Yilmaz, B. (2010). Determination of metoprolol in pharmaceutical preparations by zero-, first-, second- and third- order derivative spectrophotometric method. International journal of pharma and bio sciences, 12, 1–15. Available at: https://www.semanticscholar.org/paper/Determination-of-Metoprolol-in-Pharmaceutical-by-Y%C4%B1lmaz/a30172883195e0740dcef1b8560efb8a5b194bd0
  8. Jadhav, A. S., Tarkase, K. N., Deshpande, A. P. (2012). Quantitative determination of metoprolol succinate in bulk and tablet dosage form through comparative study of UV and derivative Spectroscopy. Der Pharmacia Lettre, 4, 763–7. Available at: https://www.scholarsresearchlibrary.com/articles/quantitative-determination-of-metoprolol-succinate-in-bulk-and-tablet-dosage-form-through-comparative-study-of-uv-and-de.pdf
  9. Rahman, N., Rahman, H., Azmi, S. N. H. (2005). Validated Kinetic Spectrophotometric Method for the Determination of Metoprolol Tartrate in Pharmaceutical Formulations. Chemical and Pharmaceutical Bulletin, 53 (8), 942–948. doi: https://doi.org/10.1248/cpb.53.942
  10. Hussain, S., Munjewar, R.R., Farooqui, M. (2012). Development and validation of a simultaneous HPLC method for quantification of amlodipine besylate and metoprolol tartrate in tablets. Journal of PharmaSciTech, 1, 1–5. Available at: http://www.pharmascitech.in/admin/php/uploads/32_pdf.pdf
  11. Singh, B. K., Patel, D. K., Ghosh, S. K. (2009). Development of Reverse-Phase HPLC method for simultaneous analysis of metoprolol succinate and hydrochlorothiazide in a tablet formulation. Tropical Journal of Pharmaceutical Research, 8, 539–543. doi: https://doi.org/10.4314/tjpr.v8i6.49401
  12. Prasad, P. H., Patel, P. M., Vijaysree, D., Reddy, Y. S., Ranjith, K. B. (2013). Simultaneous estimation of metoprolol tartrate and chlorthalidone by using RP-HPLC and method development as per ICH guidelines. Der Pharma Chemica, 5 (5), 139–143. Available at: https://www.derpharmachemica.com/pharma-chemica/simultaneous-estimation-of-metoprolol-tartrate-and-chlorthalidone-by-using-rphplc-and-method-development-as-per-ich-guid.pdf
  13. Mahaparale, S. P., Gonjari, I. D., Jayaveera, K. N. (2013). Stabılıty ındıcatıng hplc method for sımultaneous estımatıon of metoprolol succınate and telmısartan. Journal of Liquid Chromatography & Related Technologies, 36 (18), 2601–2611. doi: https://doi.org/10.1080/10826076.2012.723095
  14. Braza, A. J., Modamio, P., Lastra, C. F., Mariño, E. L. (2002). Development, validation and analytical error function of two chromatographic methods with fluorimetric detection for the determination of bisoprolol and metoprolol in human plasma. Biomedical Chromatography, 16 (8), 517–522. doi: https://doi.org/10.1002/bmc.195
  15. Chiu, F. C. K., Damani, L. A., Li, R. C., Tomlinson, B. (1997). Efficient high-performance liquid chromatographic assay for the simultaneous determination of metoprolol and two main metabolites in human urine by solid-phase extraction and fluorescence detection. Journal of Chromatography B: Biomedical Sciences and Applications, 696 (1), 69–74. doi: https://doi.org/10.1016/s0378-4347(97)00059-5
  16. Johnson, R. D., Lewis, R. J. (2006). Quantitation of atenolol, metoprolol, and propranolol in postmortem human fluid and tissue specimens via LC/APCI-MS. Forensic Science International, 156 (2-3), 106–117. doi: https://doi.org/10.1016/j.forsciint.2005.01.001
  17. Jensen, B. P., Sharp, C. F., Gardiner, S. J., Begg, E. J. (2008). Development and validation of a stereoselective liquid chromatography–tandem mass spectrometry assay for quantification of S- and R-metoprolol in human plasma. Journal of Chromatography B, 865 (1-2), 48–54. doi: https://doi.org/10.1016/j.jchromb.2008.02.006
  18. Gowda, K. V., Mandal, U., Senthamil Selvan, P., Sam Solomon, W. D., Ghosh, A., Sarkar, A. K. (2007). Liquid chromatography tandem mass spectrometry method for simultaneous determination of metoprolol tartrate and ramipril in human plasma. Journal of Chromatography B, 858 (1–2), 13–21. doi: https://doi.org/10.1016/j.jchromb.2007.07.047
  19. Albers, S., Elshoff, J.-P., Völker, C., Richter, A., Läer, S. (2004). HPLC quantification of metoprolol with solid-phase extraction for the drug monitoring of pediatric patients. Biomedical Chromatography, 19 (3), 202–207. doi: https://doi.org/10.1002/bmc.436
  20. Horyn, M., Logoyda, L. (2020). Bioanalytical method development and validation for the determination of metoprolol and meldonium in human plasma. Pharmacia, 67 (2), 39–48. doi: https://doi.org/10.3897/pharmacia.67.e50397
  21. Derzhavna farmakopeia Ukrainy. Vol. 1. Kharkiv: Derzhavne pidpryiemstvo «Ukrainskyi naukovo-ekspertnyi farmakopeinyi tsentr yakosti likarskykh zasobiv», 1128.
  22. Grizodub, A. I. (2016). Standartizovannye protcedury validatcii metodik kontrolia kachestva lekarstvennykh sredstv. Kharkіv: Derzhavne pіdpriemstvo «Ukrainskii naukovo-ekspertnii farmakopeinii tcentr iakostі lіkarskikh zasobіv», 64–67.
  23. Horyn, M., Kucher, T., Kryskiw, L., Poliak, O., Zarivna, N., Peleshok, K., Logoyda, L. (2022). Development of the spectrophotometric method for the determination of metoprolol tartrate in tablets by using bromocresol green. ScienceRise: Pharmaceutical Science, 5 (39), 55–63. doi: https://doi.org/10.15587/2519-4852.2022.266068

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Published

2022-12-30

How to Cite

Horyn, M., Kryskiw, L., Kucher, T., Poliak, O., Zarivna, N., Zahrychuk, H., Korobko, D., Peleshok, K., & Logoyda, L. (2022). Development of the spectrophotometric method for the determination of metoprolol in tablets by using bromophenol blue. ScienceRise: Pharmaceutical Science, (6(40), 29–35. https://doi.org/10.15587/2519-4852.2022.270311

Issue

No. 6(40) (2022)

Section

Pharmaceutical Science

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Copyright (c) 2022 Mariana Horyn, Liubomyr Kryskiw, Tetyana Kucher, Olha Poliak, Nadiya Zarivna, Hryhorii Zahrychuk, Dmytro Korobko, Kateryna Peleshok, Liliya Logoyda

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