ScienceRise: Pharmaceutical Science <p><em>«ScienceRise: Pharmaceutical Science» </em><em>–</em> scientific peer-reviewed journal, published 6 times a year, included in category «A» «List of scientific professional editions of Ukraine» (Сertificated by order of Ministry of Education and Science of Ukraine No. 420 from 19.04.2021).</p> <p><em>The main mission of the journal </em>– dissemination of the results of scientific research aimed at ensuring the quality of pharmaceutical assistance to the population through targeted search and modern pharmaceutical development of innovative medicines, the creation of modern quality management systems at pharmaceutical enterprises in the industry.</p> <p>In the journal<em> «ScienceRise: Pharmaceutical Science» </em>publishes research designed and implemented taking into account the Quality by design concept with widespread use of computational methods.</p> <p>The journal is intended for scientists, pharmacists, doctors, educators, and healthcare professionals.</p> <p><em>Innovations in pharmaceutical science - </em>for practical use.</p> <p><a href="">ISSN 2519-4844 </a> (print), <a href="">ISSN 2519-4852 </a>(on-line) <br /><br />Drawing up the items of the publication ethics policy of the journal «ScienceRise: Pharmaceutical Science» Editors followed the recommendations of Committee on Publication Ethics <a href="">(COPE)</a>.</p> РС ТЕСHNOLOGY СЕNTЕR en-US ScienceRise: Pharmaceutical Science 2519-4844 <p>Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.</p> Pharmacognostic study of cetraria islandica (L.) Ach. thalli made in Ukraine <p><strong>The aim </strong>was to conduct a pharmacognostic study of C. islandica thalli harvested in Ukraine.</p> <p><strong>Material and methods. </strong>The study included seven series of C. islandica thalli harvested in Ukraine. Pharmacognostic research was conducted by using generally accepted methods. Quantitative determination of total polyphenols was performed by a spectrophotometric method in terms of pyrogallol and dry raw materials following the requirements of the SPhU 2.0 monograph. The component composition of flavonoid compounds and carboxylic acids was performed by HPLC.</p> <p><strong>Results.</strong> The pharmacognostic study of 7 series of the C. islandica raw materials was performed. Morphometric parameters were determined, including the fractional composition according to the size of thalli in each series. For the first time, fractions of the mineral and foreign organic matter were separated. The content of mineral foreign matter ranged from 0.22 ± 0.01 % to 2.80 ± 0.12 %; the content of organic foreign matter ranged from 0.15 ± 0.01 % to 2.14 ± 0.11 %. Due to the total foreign matter content, 6 series of the raw materials fulfilled the requirements of the SPhU 2.0 monograph. The accordance of morphological and anatomical description of the series to the requirements of the SPhU 2.0 monograph is shown. New distinctive diagnostic features of the morphological structure were found, namely: coalescence of blades with forming a membrane and branching of cilia along the edge of blades. For the first time for the series of raw materials harvested in Ukraine, the quantitative content of the sum of polyphenols in terms of pyrogallol and dry raw materials was determined, which ranged from 1.21 ± 0.05 % to 1.73 ± 0.04 %. For the first time for C. islandica thalli, the presence of flavonoid compounds: quercetin, luteolin, kaempferol and rutin, and carboxylic acids: sinapic, trans-cinnamic and quinic acids was determined.</p> <p><strong>Conclusions.</strong> The obtained results can be used as a basis for the relevant sections of the national part of the monograph "Cetraria Iceland" in SPhU 2.0</p> Alina Shpychak Olga Khvorost Copyright (c) 2022 Alina Shpychak, Olga Khvorost 2022-10-31 2022-10-31 5 (39) 4 15 10.15587/2519-4852.2022.265019 Comparative analysis of pharmaceutical supply systems of the population of European countries according to a complex of socio-economic indicators <p><strong>The aim:</strong> to conduct an analysis of the state of functioning of pharmaceutical supply systems for the population in European countries and Ukraine based on a set of socio-economic indicators.</p> <p><strong>Materials and methods</strong>. General theoretical (historical, formal, graphic, hypothetical-deductive) and applied (organizational-economic, mathematical-statistical) research methods were used. The object of research was data that was freely available.</p> <p><strong>Results.</strong> It was established that the highest volume of the pharmaceutical market volume per inhabitant is typical for Italy (410.18 euros) and the lowest for Ukraine (53.58 euros). It has been proven that with an increase in GDP indicators, which are calculated based on purchasing power parity (PPP) per capita, the range of variation of this indicator by groups decreases, and the number of countries with a negative value of the foreign trade balance also decreases. Within groups of countries, there is a different level of dependence on the import of pharmaceutical products. In Ukraine (the first group), imports exceeded the export data of pharmaceutical products by 8.6 times. For other countries in this group (Latvia, Bulgaria, and Slovakia), imports exceeded exports by 1.3, 1.4, and 4.0 times, respectively. The highest values of the volume of foreign trade in pharmaceutical products were characteristic of the countries of the third and second groups. The undisputed leader is Germany (134,541.0 million euros), in second place is Italy (59,533.0 million euros), and in third place is France (58,568.0 million euros).</p> <p>The highest values of health care costs as a percentage of GDP (%) are typical for the countries of the third group and the lowest for the countries of the first group. For all countries, this indicator had a characteristic tendency to increase over time. Growth rates varied both across groups and within groups across countries. According to the indicator of the amount of reimbursement of the cost of drug consumption per person, there was a significant fluctuation of the data by country within the groups. The most extensive range of fluctuations was observed in the third group (284.83 euros). The highest reimbursement amounts are typical for Germany (483.53 euros) and the lowest for Bulgaria (54.25 euros). In most countries, there is a high level (50.0 % and higher) of state participation in the payment of medicine, except for Poland (36.0 %), Lithuania (34.0 %) and Latvia (37.0 %). The lowest values of money consumers spend to pay for medicines (from 13.0 % to 44.0 %) are characteristic of the countries of the third group.</p> <p><strong>Conclusions.</strong> The established peculiarities of the functioning of the pharmaceutical supply systems of European countries should not diminish the value of the state's aspirations to harmonize the processes that take place to promote medicinal products to consumers</p> Oleh Samborskyi Нanna Panfilova Yuliia Baihush Liusine Simonian Iryna Bilyk Tetiana Martyniuk Halyna Tsikhon Vitaly Chernukha Copyright (c) 2022 Oleh Samborskyi, Нanna Panfilova, Yuliia Baihush, Liusine Simonian, Iryna Bilyk, Tetiana Martyniuk, Halyna Tsikhon, Vitaly Chernukha 2022-10-31 2022-10-31 5 (39) 16 28 10.15587/2519-4852.2022.265814 Effect of the composition of emulsifiers and the dispersion medium on the properties of bases for semi-solid preparations <p><strong>The aim.</strong> To study the effect of cetostearyl alcohol (CSA) on the rheological properties of bases with different dispersion media, the release of propylene glycol (PG) from them, and the ability of these bases to absorb water.</p> <p><strong>Materials and methods</strong>. Micelles of a non-ionic surfactant and its aggregates with CSA in a mixed solvent where the structure of water prevails, mixed solvent PG – macrogol 400 (M400) and hydrophilic bases-vehicles with different dispersion media were studied. The research was carried out by the spin probe method using a probe simulating a cationic surfactant and by rotational viscometry. The microstructure of the bases was studied by optical microscopy. The in vitro release test to study the release of PG and M400 from solutions and bases was performed using vertical diffusion chambers. The content of PG and M400 in the dialysate was determined by gas chromatography according to the validated analytical procedures. The absorption of water by solutions and bases was determined by dialysis through the membrane.</p> <p><strong>Results.</strong> CSA, which was the part of the bases together with surfactants in certain ratios, was a significant factor in increasing their rheological parameters, reducing the parameters of PG release during in vitro release tests, as well as reducing water absorption. The mechanisms of such influence are different for bases with different structures of the dispersion medium. In the bases, where the structure of water prevailed, lateral phase separation occurred in the supramolecular structures of surfactant and CSA with the formation of liquid domains of surfactant and solid domains of CSA, which contributed to the formation of coagulation structures. In the mixed non-aqueous solvent PG – M400, surfactant micelles and mixed aggregates of surfactant and CSA molecules were not formed; at 25 <sup>o</sup>C, surfactants and CSA became separate dispersed phases of suspensions, which contributed to the formation of gels. When CSA was added into an aqueous solution of poloxamer 338, PG, M400 and cationic surfactant, the flow behaviour changed, and the rheological parameters increased, which led to a decrease in the release rate and extended for PG and M400 as well as in the ability to absorb water. The rate and extent of PG release from the solution were greater compared to the M400 release.</p> <p><strong>Conclusions.</strong> The addition of CSA in combination with surfactants into the bases for semi-solid preparations is a significant factor for modifying their rheological parameters, the kinetics of PG release from them, and water absorption during experiments in vitro. The mechanisms of such an effect are different and depend on the composition and structure of the dispersion medium of the base</p> Nikolay Lyapunov Elena Bezuglaya Anna Liapunova Igor Zinchenko Oleksii Liapunov Oleksii Lysokobylka Yurij Stolper Copyright (c) 2022 Nikolay Lyapunov, Elena Bezuglaya, Anna Liapunova, Igor Zinchenko, Oleksii Liapunov, Oleksii Lysokobylka, Yurij Stolper 2022-10-31 2022-10-31 5 (39) 29 45 10.15587/2519-4852.2022.266001 Research of biologically active substances of hemp seeds, hemp seed oil and hemp pomace <p>For the time being, the use of cannabis for medical purposes is more and more relevant. A review of literary sources shows that Ukrainian varieties of hemp are insufficiently studied. Therefore, the variety "Glesia" was chosen for the study, as it is the most promising Ukrainian variety. Fatty oil from hemp seeds is the leading pharmaceutical and food product produced from this raw material in Ukraine. During its production, the pomace remains, which is used for feeding animals. At the same time, it still contains many other BAS and can be a valuable raw material for creating pharmaceutical products. Therefore, developing technologies for the complex processing of this raw material is an urgent task of modern pharmaceutical science.</p> <p><strong>The</strong> <strong>aim</strong> <strong>of</strong> <strong>this</strong> <strong>work</strong> was a phytochemical study of biologically active substances of hemp seeds, hemp seed oil and hemp pomace in order to develop the new phytoremedies.</p> <p><strong>Materials</strong> <strong>and</strong> <strong>methods</strong>. Non-narcotic hemp seeds of the "Glesia" variety, hemp seed oil and hemp pomace were the objects of research. The elemental analysis was made using inductively coupled plasma atomic emission spectrometry - iCAP 7000 Duo; the study of amino acids was made using ion exchange chromatography; the study of fatty acids was made using gas-liquid chromatography. In addition, the content of vitamin E (α-, β- and γ-tocopherols) was studied using high-performance liquid chromatography (HPLC) with UV detection; the content of protein was studied using A.I. Ermakov method in O.O. Sozinov and F.O. Poperelia modification.</p> <p><strong>R</strong><strong>esearch r</strong><strong>esults</strong>. The analysis of the qualitative characteristics of the obtained fatty oils shows that all indicators met the requirements of the State Standard of Ukraine. For the first time, the transition of macro- and microelements from hemp seeds of the "Glesia" variety into fatty oil was determined, and their residue in the pomace was established. The content of 16 amino acids was determined. The content of saturated and unsaturated fatty acids in oil samples was established. The content of α- β- γ-tocopherol in hemp seeds, hemp oil and hemp pomace was investigated using GC / MS. It was found that the protein content in the pomace was in the range of 32.8 – 34.6 %.</p> <p><strong>Conclusions.</strong> We conducted a complex study of biologically active substances of non-narcotic hemp seeds of the "Glesia" variety that was harvested in 2019 and 2020, the hemp oil and hemp pomace. It was established that the content of macro- and microelements in the studied raw material of Cannabis sativa L. corresponds to the following order: Ca&gt; Mg&gt; Si&gt; Fe&gt; Al&gt; Mn&gt; Zn&gt; Sr&gt; B&gt; Cu&gt; Ba&gt; Cr and Ni&gt; Se&gt; Co&gt; Mo&gt; Cd&gt; Be&gt; I&gt; Pb. The content of 16 amino acids was determined. Of them, 7 amino acids are essential (leucine, valine, threonine, lysine, methionine, isoleucine, phenylalanine), 2 amino acids are essential for children (histidine and arginine), and 7 amino acids are replaceable (alanine, tyrosine, proline, glycine, glutamic and aspartic acids). It was found that the main fatty acids of all samples were linoleic, oleic and linolenic. The content of α- and γ-tocopherol predominated in the studied samples. Hemp seeds of the "Glesia" variety and hemp pomace contain protein. The protein content in the pomace ranged from 32.8 to 34.6 %</p> Oksana Struk Andrii Grytsyk Mikola Mikitin Mykhailo Obodianskyi Tetiana Stasiv Sofiia Svirska Copyright (c) 2022 Oksana Struk, Andrii Grytsyk, Mikola Mikitin, Mykhailo Obodianskyi, Tetiana Stasiv, Sofiia Svirska 2022-10-31 2022-10-31 5 (39) 46 54 10.15587/2519-4852.2022.241249 Development of the spectrophotometric method for the determination of metoprolol tartrate in tablets by using bromocresol green <p><strong>The aim of the work</strong> was to develop and validate a spectrophotometric method for determining metoprolol tartrate in tablets based on the reaction with bromocresol green (BCG).</p> <p><strong>Material and methods. </strong>Analytical equipment: two-beam UV-visible spectrophotometer Shimadzu model -UV 1800 (Japan), software UV-Probe 2.62, laboratory electronic balance RAD WAG AS 200/C, pH-meter И-160МИ. The following APIs, dosage forms, reagents and solvents were used in the work: pharmacopoeial standard sample (CRS) of metoprolol tartrate (Sigma-Aldrich, (≥ 98 %, HPLC)), BCG (Sigma-Aldrich, (≥ 98 %, HPLC)), "Metoprolol" tablets 50 mg (Kyivmedpreparat, series 0035415), "Metoprolol" 100 mg (Farmak, series 30421), methanol (Honeywell, (≥ 99.9 %, GC)), ethanol (Honeywell, (≥ 99.9 %, GC)), chloroform (Honeywell, (≥ 99.9 %, GC)), acetonitrile (Honeywell, (≥ 99.9 %, GC)), and ethyl acetate (Honeywell, (≥ 99.7 %, GC)).</p> <p><strong>Results and discussion. </strong>A spectrophotometric method was developed for determining metoprolol tartrate by reaction with BCG in a methanol solution using the absorption maximum at a wavelength of 624 nm. Stoichiometric ratios of reactive components were established, which were 1:1. The developed method for the quantitative determination of metoprolol tartrate was validated following the requirements of the SPhU. The analytical method was linear in the concentration range of 5.47-38.30 μg/mL. The limit of detection and quantification were 0.41 μg/mL and 1.24 μg/mL, respectively. According to the «greenness» pictogram of the analytical method using the AGREE method, the score was 0.79, which indicates that the proposed spectrophotometric method for the determination of metoprolol was developed in compliance with the principles of «green» chemistry.<br /><strong>Conclusions. </strong>A spectrophotometric method for determining metoprolol tartrate in tablets based on the reaction with BCG in compliance with the principles of «green» chemistry has been developed and validated. Furthermore, the developed method for the quantitative determination of metoprolol tartrate was validated following the requirements of the SPhU. In summary, the developed method has a low negative impact on the environment and can be applied for routine pharmaceutical analysis</p> Mariana Horyn Tetyana Kucher Liubomyr Kryskiw Olha Poliak Nadiya Zarivna Kateryna Peleshok Liliya Logoyda Copyright (c) 2022 Mariana Horyn, Tetyana Kucher, Liubomyr Kryskiw, Olha Poliak, Nadiya Zarivna, Kateryna Peleshok, Liliya Logoyda 2022-10-31 2022-10-31 5 (39) 55 63 10.15587/2519-4852.2022.266068 Broad-purpose antimicrobial chlorine-active polymers: suppression of multidrug-resistant microorganisms and microbial penetration resistance <p><strong>The aim of the work </strong>was to evaluate the antimicrobial activity of polymeric materials with immobilized N-Chlorosulfonamide groups against multidrug-resistant hospital strains of common microorganisms and to determine the resistance to microbial penetration of these materials.</p> <p><strong>Materials and methods:</strong> the studied samples were copolymers of styrene with divinylbenzene in the form of staple fibre and non-woven fabric with immobilized</p> <p>N-Chlorosulfonamide groups of various structures. Hospital strains of microorganisms have been isolated from clinical material; their antibiotic sensitivity has been determined by the Kirby-Bauer method. The agar diffusion method determines the antimicrobial activity of the polymers. Resistance to microbial penetration of samples of non-woven fabric has been determined by the membrane filtration method.</p> <p><strong>Results:</strong> polymer samples have been synthesized with immobilized N-Chlorosulfonamide groups in the Na- and H-forms, and with the N, N-dichlorosulfonamide group, with chlorine concentration range 3.7 - 12.5 %. All samples demonstrated pronounced antimicrobial activity against both standard and hospital strains. Due to the higher specific surface area, staple fibre is generally more efficient. An increase in the zone of inhibition of the growth of microorganisms was observed with an increase in the concentration of immobilized chlorine. All the studied fabric samples are impermeable to S. aureus. The control samples containing the free sulfonamide group did not show antimicrobial properties.</p> <p><strong>Conclusions</strong><strong>:</strong> synthesized chlorine-active polymers have a pronounced antimicrobial activity against multidrug-resistant microorganisms, demonstrate high resistance to microbial penetration and therefore are promising for creating a wide range of medical products on their basis: dressings, protective masks, antimicrobial filters, etc.</p> Bohdan Murashevych Iryna Koshova Elena Surmasheva Dmitry Girenko Vasyl Chuiko Dmytro Stepanskyi Copyright (c) 2022 Bohdan Murashevych, Iryna Koshova, Elena Surmasheva, Dmitry Girenko, Vasyl Chuiko, Dmytro Stepanskyi 2022-10-31 2022-10-31 5 (39) 64 73 10.15587/2519-4852.2022.266171 Test anti-aging activity in a face scrub preparation that contains coffee-grade active charcoal (Coffea arabica L.) with the addition of vitamin E <p>Ageing can be caused by accumulating excess dead skin cells on the skin's surface. Coffee grounds are a by-product of the coffee brewing process. Coffee grounds can be used as activated charcoal in face scrub preparations.</p> <p><strong>The purpose of this study was</strong> to formulate and test the effectiveness of anti-ageing face scrub preparations of coffee grounds activated charcoal (Coffea arabica L.) with the addition of vitamin E.</p> <p><strong>Material and methods:</strong> the research method includes processing coffee grounds in the form of drying and making activated charcoal, then formulating into face scrub preparations with a concentration of 1 %; 2 %, 3 % with vitamin E, 5 % and without vitamin E and blanc (without activated charcoal). Evaluation of face scrub preparations includes homogeneity test, stability test (odour, colour, pH and consistency), cycling test, dispersion test, viscosity test, centrifugation test, hedonic test, irritation test, and effectiveness test (moisture, pores, stains, wrinkles) on facial skin using a skin analyser on 24 volunteers with application to the face once a week for 4 weeks. The experimental data were analysed using the SPSS 22 program.</p> <p><strong>Results:</strong> they showed that all face scrub preparations were in the form of cream with grey-black granules, coffee-scented, homogeneous, stable for 12 weeks of storage at room temperature (20-25°C), pH value (5.0-6.0), had a spreading power of 5-7 cm, viscosity (3760-3996 mPa's), no separation occurs in the centrifugation test and does not irritate facial skin. The results of the hedonic test, the most preferred formulation, was the F3 preparation. The effectiveness test results were an increase in humidity of 27 %, a decrease in pore size of 35.8 %, stains of 40 % and wrinkles of 37.6 %.</p> <p><strong>Conclusion:</strong> from the results of the study, it can be concluded that the face scrub preparation of activated charcoal coffee grounds (Coffea arabica L.) with a concentration of 3 % and vitamin E 5 % is a formula that meets the evaluation of the preparation and measures the effectiveness of anti-ageing</p> Dara Sukma Ratmelya Julia Reveny Urip Harahap Copyright (c) 2022 Dara Sukma Ratmelya, Julia Reveny, Urip Harahap 2022-10-31 2022-10-31 5 (39) 74 82 10.15587/2519-4852.2022.265402 SGLT-2 inhibitors as potential anticonvulsants: empagliflozin, but not dapagliflozin, renders a pronounced effect and potentiates the sodium valproate activity in pentylenetetrazole-induced seizures <p>On the way to the search for effective adjuvant medicines for epilepsy treatment, antidiabetic medicines such as sodium-glucose cotransporter-2 inhibitors, which are expressed not only in the kidneys but also in the brain, attract attention. From previous studies, it is known that dapagliflozin improves electroencephalographic parameters in rats on the model of pentylenetetrazole-induced seizures. However, the anticonvulsant potential of other medicines from this group needs to be clarified.</p> <p>The aim of the study is to estimate the effect of empagliflozin, dapagliflozin per se and their combinations with sodium valproate on pentylenetetrazole-induced seizures, as well as on muscle tone and motor coordination in mice.</p> <p><strong>Material and methods. </strong>42 random-bred male albino mice weighing 24-28 g were used in the experiments. Empagliflozin (20 mg/kg) and dapagliflozin (50 mg/kg) were administered intragastrically for 3 days. The classic anticonvulsant sodium valproate (150 mg/kg) per se, in combination with the medicines mentioned above, was administered in a similar regimen. On the second day, 30 minutes after administering all medicines, their effect on muscle tone and coordination of movements was determined in the rotarod test. On the third day, 30 minutes after the last administration of the medicines, their effect on pentylenetetrazole-induced (80 mg/kg subcutaneously) seizures was studied.</p> <p><strong>Results. </strong>For the first time, a pronounced anticonvulsant effect of empagliflozin was established both when used alone (a significant increase in latency of the convulsions and a decrease in lethality by 43 %) and especially in combination with sodium valproate (a significant increase in latency of the convulsions, a decrease in the number and severity of seizures and a decrease in lethality by 83 %), as well as the absence of a muscle relaxant effect in both cases. Dapagliflozin has neither its anticonvulsant properties nor its effect on the action of sodium valproate. However, this medicine caused muscle relaxation, especially when combined with sodium valproate.</p> <p><strong>Conclusions.</strong> The results suggest that empagliflozin, unlike dapagliflozin, has a high potential as an adjuvant medicine in treating epilepsy, as it enhances the efficacy of the classic anticonvulsant sodium valproate without muscle relaxant side effects</p> Vadim Tsyvunin Sergiy Shtrygol’ Ihnat Havrylov Diana Shtrygol’ Artur Reus Copyright (c) 2022 Vadim Tsyvunin, Sergiy Shtrygol’, Ihnat Havrylov, Diana Shtrygol’, Artur Reus 2022-10-31 2022-10-31 5 (39) 83 90 10.15587/2519-4852.2022.266065 Research on the phenolic profile, antiradical and anti-inflammatory activity of a thick hydroalcoholic feverfew (Tanacetum parthenium L.) herb extract <p><strong>The aim</strong> – to study the phenolic complex of a thick hydroalcoholic extract of the feverfew (Tanacetum parthenium (L.) herb (FTHAE), its antiradical activity and anti-inflammatory properties in a model of carrageenan and histamine oedema.</p> <p><strong>Materials and methods.</strong> The studied extract was obtained from the Tanacetum parthenium herb, collected in Sumy and Poltava regions of Ukraine during the period of mass flowering (June-August): degree of grinding of raw materials 2.0-3.0 mm, extraction temperature - 25 °C, extractant – 70 % ethanol, raw material/extractant ratio – 1:12, infusion time – 12 hours, multiplicity of extractions – 3. HPLC and spectrophotometric methods were used to determine the composition and amount of phenolic compounds of FTHAE. HPLC analysis was performed using a “Waters e2695 Alliance system” (Waters, Milford, MA, USA) with a photodiode array detector “Waters 2998” according to the HPLC–PDA method for phenolic compounds. The scavenging of ABTSA radical cation evaluated the radical scavenging activity. In addition, the anti-inflammatory properties of FTHAE were studied on carrageenan and histamine paw oedema in rats. Anti-inflammatory activity (AIA) was evaluated as the ability to reduce oedema compared to the control pathology. FTHAE was used at a dose of 50 mg/kg.</p> <p><strong>The results.</strong> The content of the sum of hydroxycinnamic acids in the obtained extract was determined by spectrophotometry, which was 13.92±0.02 % and the content of the sum of flavonoids – 5.16±0.03 %. The content of 12 compounds with a total amount of 72432.09µg/g was identified and determined by HPLC. The dominant compounds were hydroxycinnamic acids, namely 3,4-dicaffeoylquinic, 4,5-dicaffeoylquinic and сhlorogenic acids. The antiradical activity of the extract was 620.19±4.53µmol/g. On the model of carrageenan oedema, the maximum effect of oedema suppression was 71.0-73.2 %. In the model of histamine oedema, the anti-inflammatory effect of the extract was 57.8; 51.8; and 49.1 % for 30 minutes, 1 and 1.5 hours of oedema, respectively. The severity of the anti-inflammatory activity of the extract during the first hour is not inferior to the diclofenac sodium, quercetin and loratadine.</p> <p><strong>Conclusions.</strong> Due to the HPLC method, 12 compounds were determined to cause antiradical activity, among which chlorogenic acid and rutin were identified.</p> <p>The studied extract has a pronounced anti-inflammatory effect, which is due to the antiradical properties of the extract and its inhibitory effect on inflammatory mediators</p> Oksana Mishchenko Inna Kyrychenko Tetiana Gontova Kateryna Kalko Karyna Hordiei Copyright (c) 2022 Oksana Mishchenko, Inna Kyrychenko, Tetiana Gontova, Kateryna Kalko, Karyna Hordiei 2022-10-31 2022-10-31 5 (39) 91 99 10.15587/2519-4852.2022.266400 Potential antioxidants of secondary metabolite isolates ethyl acetate fraction Coleus amboinicus Lour. Leaves. <p><strong>The aim</strong> of the study was to isolate and characterize secondary metabolites that have the potential as antioxidants from the ethyl acetate fraction of the leaves of Coleus amboinicus, L. (C. amboinicus).</p> <p><strong>Materials and methods.</strong> Purification of the ethyl acetate fraction of C. amboinicus using gravity column chromatography with a stationary phase (silica gel, Merck) and a mobile phase with a solvent ratio of n-hexane (Merck) and ethyl acetate (Merck). Examining isolates includes physical (colour, shape, and melting point). Qualitative purity detection by TLC at 254 nm and 366 nm wavelengths. Structural analysis of metabolites with UV-Vis spectrometer (Spectronic 3000, Genesis 10, Japan), FT-IR(KBr) (Shimadzu IR Prestige-21, Japan), NMR spectrometer (JEOL spectrometer, Japan) operating at 500 MHz (1H-NMR) and 125 MHz (13C-NMR), and Shimadzu's GC-MS (QP-2010S Shimadzu, Japan) and determination of potential antioxidant activity using the DPPH method.</p> <p><strong>Results.</strong> The secondary metabolite compounds were isolated in the form of yellow crystals with a melting point of 232-233 °C and Rf values of 0.86 and 0.56, which TLC monitored at a solvent ratio of n-hexane and ethyl acetate 6:4 and 8:2. Spectronic analysis with a UV-Vis Spectrometer showed two electron absorbances, namely a wavelength of 210 nm indicating methanol solvent and 272 nm isolate. The absorbance of functional groups at wave numbers 3379 cm-1 (-OH; hydroxy), 2931 cm-1 (-CH; aliphatic), 1735 cm-1 (-C=O; carbonyl ketone), 1234 cm-1 (-CO-; methoxy) and 1643 cm-1 (-C=C-; alkene). GC-MS analysis obtained two absorbance peaks, (1) the first retention time of 6.658 minutes (3.95 %) and (2) the second retention time of 9.001 minutes (96.05 %). Structural analysis with 1H&amp;13C-NMR showed 28 types of protons and 22 types of carbon. The antioxidant activity potential test showed an activity value (IC<sub>50</sub>) of 338.54 ppm.</p> <p><strong>Conclusion.</strong> The structure of the isolated secondary metabolite compound is 16-acetoxy-7α-hydroxyroyleanone (syn. 16-acetoxyhorminone) and has the potential as an antioxidant.</p> Kasta Gurning Winarto Haryadi Copyright (c) 2022 Kasta Gurning, Winarto Haryadi 2022-10-31 2022-10-31 5 (39) 100 105 10.15587/2519-4852.2022.266401