A COMPARATIVE STUDY ON THE CYTOTOXIC EFFECT OF DIFFERENT CONCENTRATIONS OF HYDROGEN PEROXIDE AND CARBAMIDE PEROXIDE ON THE SURVIVAL OF HUMAN DENTAL PULP STEM CELLS IN VITRO
DOI:
https://doi.org/10.32461/2226-3209.3.2018.171945Ключові слова:
Stem cells, Dental pulp, Apoptosis, Hydrogen peroxide, Carbamide peroxideАнотація
Abstract. Background: Tooth discoloration due to different causes (systemic or acquired) is one of the major issues that has involved the dentists to itself. up to now, dental bleaching has been reported as the most conservative method of treating teeth discoloration. In most bleaching methods, hydrogen peroxide and carbamide peroxide derivatives are used at different concentrations. Considering that these materials are oxidative materials, in this study, we are trying to evaluate and compare the toxicity of different concentrations of these two materials on dental pulp stem cells. Materials and
methods: Human dental pulp stem cells (DPSCs) were extracted and cultured, and they were treated with 50, 100, 180, 250 and 300 μM concentrations of hydrogen peroxide and 1.9, 0.95, 0.71, 0.47 and 0.24 mg/ml concentrations of carbamide peroxide. Metabolic activity of the cells was evaluated by MTT test. Objective: The purpose of this study was to evaluate and compare the cytotoxic effects of various concentrations of hydrogen peroxide and carbamide peroxide on the survival of human dental pulp stem cells. Results: The results showed that viability of treated cells with hydrogen peroxide and
carbamide peroxide has decreased at high concentrations. IC50 was at 180 μM concentration from HP and 1.9 mg concentration of CP, which was statistically significant (P<0.05). Conclusion: The results of our studies showed that these two materials affect the growth of stem cells in a dose-dependent manner. These materials, at low concentrations, cause a esistance to oxidative stress, however, at high concentrations, they have toxic effects on cell growth. The fatal effect of hydrogen peroxide on cells is much more than the effect of carbamide peroxide on the same cell line. It can be concluded
that carbamide peroxide has less side effects than hydrogen peroxide and its use at a lower dose compared to hydrogen peroxide can be a good method for tooth bleaching.
Keywords: Stem cells, Dental pulp, Apoptosis, Hydrogen peroxide, Carbamide peroxide
Посилання
Tredwin CJ, Naik S, Lewis NJ, Scully C. Hydrogen peroxide tooth-whitening (bleaching) products: review of adverse
effects and safety issues. British dental journal. 2006 Apr 8;200(7):371-6. PubMed PMID: 16607324.
Nada OA, El Backly RM. Stem Cells From the Apical Papilla (SCAP) as a Tool for Endogenous Tissue Regeneration.
Frontiers in bioengineering and biotechnology. 2018;6:103-. PubMed PMID: 30087893.
Féliz-Matos L, Hernández LM, Abreu N. Dental Bleaching Techniques; Hydrogen-carbamide Peroxides and Light
Sources for Activation, an Update. Mini Review Article. The open dentistry journal. 2015;8:264-8. PubMed PMID:25646134.
Abouassi T, Wolkewitz M, Hahn P. Effect of carbamide peroxide and hydrogen peroxide on enamel surface: an in vitro
study. Clinical oral investigations. 2011 Oct;15(5):673-80. PubMed PMID: 20623152.
Betteridge DJ. What is oxidative stress? Metabolism: clinical and experimental. 2000 Feb;49(2 Suppl 1):3-8. PubMed
PMID: 10693912.
Demarco FF, Conde MCM, Cavalcanti BN, Casagrande L, Sakai VT, Nör JE. Dental pulp tissue engineering. Brazilian
dental journal. 2011;22(1):3-13. PubMed PMID: 21519641.
Biehl JK, Russell B. Introduction to stem cell therapy. The Journal of cardiovascular nursing. 2009 Mar-Apr;24(2):98-105. PubMed PMID: 19242274.
Rao RR, Johnson AV, Stice SL. Cell surface markers in human embryonic stem cells. Methods in molecular biology.
;407:51-61. PubMed PMID: 18453248.
Vazin T, Freed WJ. Human embryonic stem cells: derivation, culture, and differentiation: a review. Restorative
neurology and neuroscience. 2010;28(4):589-603. PubMed PMID: 20714081. Pubmed Central PMCID: 2973558.
Potdar PD, Jethmalani YD. Human dental pulp stem cells: Applications in future regenerative medicine. World journal
of stem cells. 2015;7(5):839-51. PubMed PMID: 26131314. Epub 06/26.
Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in
vivo. Proceedings of the National Academy of Sciences of the United States of America. 2000 Dec 5;97(25):13625-30.
PubMed PMID: 11087820. Pubmed Central PMCID: 17626.
Sulieman M, Addy M, MacDonald E, Rees JS. The effect of hydrogen peroxide concentration on the outcome of tooth
whitening: an in vitro study. Journal of dentistry. 2004 May;32(4):295-9. PubMed PMID: 15053912.
Matis BA, Cochran MA, Eckert G, Carlson TJ. The efficacy and safety of a 10% carbamide peroxide bleaching gel.
Quintessence international. 1998 Sep;29(9):555-63. PubMed PMID: 9807138.
Soares DG, Goncalves Basso F, Hebling J, de Souza Costa CA. Effect of hydrogen-peroxide-mediated oxidative stress
on human dental pulp cells. Journal of dentistry. 2015 Jun;43(6):750-6. PubMed PMID: 25527247.
Soares DG, Basso FG, Hebling J, de Souza Costa CA.(2015c) Effect of hydrogen-peroxide-mediated oxidative stress on
human dental pulp cells. J Dent 43:750-756
Benetti AR, Valera MC, Mancini MN, Miranda CB, Balducci I. In vitro penetration of bleaching agents into the pulp
chamber. International endodontic journal. 2004 Feb;37(2):120-4. PubMed PMID: 14871178.
Gao Z, Yang Q, Ma X, Becker B, Li K, Zhou F, et al. Men Who Compliment a Woman's Appearance Using
Metaphorical Language: Associations with Creativity, Masculinity, Intelligence and Attractiveness. Frontiers in
psychology. 2017;8:2185-. PubMed PMID: 29312054.
Fornaini C, Lagori G, Merigo E, Meleti M, Manfredi M, Guidotti R, Serraj A, Vescovi P. Analysis of shade,
temperature and hydrogen peroxide concentration during dental bleaching: in vitro study with the KTP and diode
lasers. Lasers in medical science. 2013 Jan 1;28(1):1-6.
Kassis C, Zogheib T. Extrinsic tooth discoloration, an updated review. Dental Tribune. 2015;6:5B-8B.
Buchalla W, Attin T. External bleaching therapy with activation by heat, light, or laser:a systematic review. Dent Mater. 2007; 23(5): 586-96.
Freedman GA. Contemporary Esthetic Dentistry-E-Book. Elsevier Health Sciences; 2011 Dec 15.
Shahabi S, Assadian H, Mahmoudi Nahavandi A, Nokhbatolfoghahaei H. Comparison of Tooth Color Change After
Bleaching With Conventional and Different Light-Activated Methods. Journal of lasers in medical sciences. 2018
Winter;9(1):27-31. PubMed PMID: 29399308. Epub 12/26.
Kabir R, Gupta M, Aggarwal A, Sharma D, Sarin A, Kola MZ. Imperative role of dental pulp stem cells in regenerative
therapies: a systematic review. Nigerian journal of surgery : official publication of the Nigerian Surgical Research Society. 2014 Jan;20(1):1-8. PubMed PMID: 24665194. Pubmed Central PMCID: 3953626.
Hanks CT, Fat JC, Wataha JC, Corcoran JF. Cytotoxicity and dentin permeability of carbamide peroxide and hydrogen
peroxide vital bleaching materials, in vitro. Journal of dental research. 1993 May;72(5):931-8. PubMed PMID: 8501291.
Di Scipio F, Sprio AE, Folino A, Carere ME, Salamone P, Yang Z, et al. Injured cardiomyocytes promote dental pulp mesenchymal stem cell homing. Biochimica et biophysica acta. 2014 Jul;1840(7):2152-61. PubMed PMID: 24631652.
Bansal R, Jain A. Current overview on dental stem cells applications in regenerative dentistry. Journal of natural
science, biology, and medicine. 2015 Jan-Jun;6(1):29-34. PubMed PMID: 25810631.
Benetti F, Gomes-Filho JE, Ferreira LL, Ervolino E, Briso ALF, Sivieri-Araujo G, et al. Hydrogen peroxide induces
cell proliferation and apoptosis in pulp of rats after dental bleaching in vivo: Effects of the dental bleaching in pulp.
Archives of oral biology. 2017 Sep;81:103-9. PubMed PMID: 28500951.
Kabbach W, Zezell DM, Pereira TM, Albero FG, Clavijo VR, de Andrade MF. A thermal investigation of dental bleaching in vitro. Photomedicine and laser Surgery. 2008 Oct 1;26(5):489-93.
Malkondu Ö, Yurdagüven H, Say EC, Kazazoğlu E, Soyman M. Effect of bleaching on microhardness of esthetic
restorative materials. Operative dentistry. 2011 Mar;36(2):177-86.
Mokhlis GR, Matis BA, COCHRAN MA, ECKERT GJ. A clinical evaluation of carbamide peroxide and hydrogen peroxide whitening agents during daytime use. The Journal of the American Dental Association. 2000 Sep 1;131(9):1269-77.
Gökay O, Yilmaz F, Akin S, Tunçbìlek M, Ertan R. Penetration of the pulp chamber by bleaching agents in teeth restored with various restorative materials. Journal of Endodontics. 2000 Feb 1;26(2):92-4.
Schiavoni RJ, Turssi CP, Rodrigues JA, Serra MC, Pécora JD, Froener IC. Effect of bleaching agents on enamel
permeability. American journal of dentistry. 2006 Oct;19(5):313-6.
Matis BA, Cochran MA, Eckert GJ, Matis JI. In vivo study of two carbamide peroxide gels with different desensitizing
agents. Operative Dentistry. 2007 Nov;32(6):549-55.
Basting RT, Amaral FL, França FM, Flório FM. Clinical comparative study of the effectiveness of and tooth sensitivity to 10% and 20% carbamide peroxide home-use and 35% and 38% hydrogen peroxide in-office bleaching materials containing desensitizing agents. Operative dentistry. 2012 Sep;37(5):464-73.
Forner L, Amengual J, Liena C, Riutord P. Therapeutic effectiveness of a new enzymatic bleaching dentifrice. Eur J
Esthet Dent. 2012 Mar 1;7(1):62-70.
Akal N, Over H, Olmez A, Bodur H. Effects of carbamide peroxide containing bleaching agents on the morphology and
subsurface hardness of enamel. Journal of Clinical Pediatric Dentistry. 2001;25(4):293-6.
Basting RT, Rodrigues Junior Al. Serra MC: The effects of 10% carbamide peroxide bleaching materials on microhardness of sound and demineralized enamel and dentin in situ. Oper Dent 2001;26:531-539.
Haywood VB, Leech T, Heymann HO, Crumpler D, Bruggers K: Night – guard vital bleaching: Effect on enamel
surface texture diffusion. Quintessence Int 1990;21:801-804.
Tredwin CJ, Naik S, Lewis NJ, Scully CB. Hydrogen peroxide tooth-whitening (bleaching) products: review of adverse
effects and safety issues. British dental journal. 2006 Apr 8;200(7):371.
Walsh LJ. Safety issues relating to the use of hydrogen peroxide in dentistry. Australian dental journal. 2000 Dec;45(4):257-69.
Ernst CP, Marroquin BB, Willershausen-Zönnchen B. Effects of hydrogen peroxide-containing bleaching agents on the
morphology of human enamel. Quintessence International. 1996 Jan 1;27(1).
NASIRI, Mahdi; REZGHI, M.; MINAEI, B. Fuzzy dynamic tensor decomposition algorithm for recommender system.
UCT Journal of Research in Science, Engineering and Technology, 2014; 2: 52-55.
Maginga TJ, Nordey T, Ally M. Extension System for Improving the Management of Vegetable Cropping Systems.
Journal of Information Systems Engineering & Management. 2018;3(4), 29.
##submission.downloads##
Номер
Розділ
Ліцензія
Автори, які публікуються у цьому журналі, погоджуються з наступними умовами:
1. Автори залишають за собою право на авторство своєї роботи та передають журналу право першої публікації цієї роботи на умовах ліцензії Creative Commons Attribution License, котра дозволяє іншим особам вільно розповсюджувати опубліковану роботу з обов'язковим посиланням на авторів оригінальної роботи та першу публікацію роботи у цьому журналі.
2. Автори мають право укладати самостійні додаткові угоди щодо неексклюзивного розповсюдження роботи у тому вигляді, в якому вона була опублікована цим журналом (наприклад, розміщувати роботу в електронному сховищі установи або публікувати у складі монографії), за умови збереження посилання на першу публікацію роботи у цьому журналі.
3.Політика журналу дозволяє і заохочує розміщення авторами в мережі Інтернет (наприклад, у сховищах установ або на особистих веб-сайтах) рукопису роботи, як до подання цього рукопису до редакції, так і під час його редакційного опрацювання, оскільки це сприяє виникненню продуктивної наукової дискусії та позитивно позначається на оперативності та динаміці цитування опублікованої роботи.
