Molecular ang genetic analysis of polymorphous variants val158met of the comt gene in children who need dental treatment under the general ahesthesia

Authors

  • Галина Мироновна Солонько Danylo Halytskyy Lviv National Medical University 69 Pekarska str., Lviv, Ukraine, 79010, Ukraine
  • Ніна Іванівна Смоляр Danylo Halytskyy Lviv National Medical University 69 Pekarska str., Lviv, Ukraine, 79010, Ukraine
  • Марта Ярославівна Тиркус «Institute of congenital defects of National Academy of Medical Sciences of Ukraine». 32 Lysenko Street, Lviv, Ukraine, 79010, Ukraine
  • Галина Василівна Макух «Institute of congenital defects of National Academy of Medical Sciences of Ukraine». 32 Lysenko str., Lviv, Ukraine, 79010, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.47684

Keywords:

Children, dental treatment, fear, general anesthesia, molecular and genetic analysis, COMT gene, allelic polymorphism.

Abstract

The study of an influence of polymorphism of the СОМТ 2 gene on forming the negative emotions caused by the dental intervention allows to explain an appearance of a fear in children.

Aim. An analysis of polymorphous variants Val158Met of the СОМТ gene in children who underwent the dental treatment under the general anesthesia.   

Materials and methods. There were studied the patterns of DNA taken from 31 children who underwent the dental treatment under anesthesia and from 30 children who had not fear of dentist and were treated in usual conditions.  

Results. As the result of the molecular and genetic research of polymorphous locus 472 G>A (Val158Met) of the COMT gene in the group of children with an unconquerable fear of dental treatment comparing with the control one of children who agreed to cooperation were detected the probabilistic differences in distribution of genotypes and alleles (р=0,02). There was established that the presence of Met/Met genotype of the locus 472 G>A (Val158Met) of the COMT gene is a genetic factor that increase the risk of the development of the fear of treatment that can be explained by the higher pain sensitivity in children with such genotype. So the genetic test of the 472 G>A (Val158Met) locus of the COMT gene can be recommended to detect the groups of children that dental treatment under the general anesthesia can be recommended for.

Author Biographies

Галина Мироновна Солонько, Danylo Halytskyy Lviv National Medical University 69 Pekarska str., Lviv, Ukraine, 79010

Candidat of Medical Science, Associate Professor

Department of Paediatric Dentistry

Ніна Іванівна Смоляр, Danylo Halytskyy Lviv National Medical University 69 Pekarska str., Lviv, Ukraine, 79010

Doctor of Medical Science, Professor

Department of Conservative Dentistry, Faculty of Postgraduate Education

Марта Ярославівна Тиркус, «Institute of congenital defects of National Academy of Medical Sciences of Ukraine». 32 Lysenko Street, Lviv, Ukraine, 79010

Candidate of Biological Sciences, leading research scientist of congenital defects diagnostic department 

Галина Василівна Макух, «Institute of congenital defects of National Academy of Medical Sciences of Ukraine». 32 Lysenko str., Lviv, Ukraine, 79010

Doctor of Biological Sciences, leading research scientist of congenital defects diagnostic department 

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Published

2015-08-20

Issue

Vol. 8 No. 3(13) (2015)

Section

Medical

License

Copyright (c) 2015 Галина Мироновна Солонько, Ніна Іванівна Смоляр, Марта Ярославівна Тиркус, Галина Василівна Макух

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