Isolation and characterization of recombinant yeast glycerol dehydrogenase

Authors

  • Марія Миколаївна Синенька Institute of Cell Biology, NAS of Ukraine, 14/16 Drahomanov Str., Lviv, Ukraine, 79005, Ukraine
  • Галина Зуфарівна Гайда Institute of Cell Biology, NAS of Ukraine, 14/16 Drahomanov Str., Lviv, Ukraine, 79005, Ukraine
  • Галина Миколаївна Клепач Drohobych Ivan Franko State Pedagogical University, 23 Shevchenкo Str., Drohobych, Lviv region, Ukraine, 82100, Ukraine
  • Марія Федорівна Іваш Institute of Cell Biology NAS of Ukraine Drahomanov Str. 14/16, Lviv, Ukraine, 79005, Ukraine
  • Михайло Васильович Гончар Institute of Cell Biology NAS of Ukraine, 14/16 Drahomanov str., Lviv, Ukraine, 79005, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.48369

Keywords:

enzymes of microbial origin, yeast glycerol dehydrogenase, glycerol, recombinant strain-producer, properties of enzyme

Abstract

Enzymes of microbial origin are widely used as components of the analytical test-kits for clinic and industry. A key component of the test-kits for glycerol monìtoring is glycerol dehydrogenase (GDH). Because of the lack of domestic enzyme, the development of cost-effective technologies for its obtaining is actual. As a result of the research a highly purified GDH preparation was isolated from recombinant yeast cells and enzyme’s properties were characterized

Author Biographies

Марія Миколаївна Синенька, Institute of Cell Biology, NAS of Ukraine, 14/16 Drahomanov Str., Lviv, Ukraine, 79005

Department of analytical biotechnology, engineer

Галина Зуфарівна Гайда, Institute of Cell Biology, NAS of Ukraine, 14/16 Drahomanov Str., Lviv, Ukraine, 79005

Phylosophy doctor, chemistry, senior scientist

Department of analytical biotechnology, senior scientist

Галина Миколаївна Клепач, Drohobych Ivan Franko State Pedagogical University, 23 Shevchenкo Str., Drohobych, Lviv region, Ukraine, 82100

Candidate of Biological Sciences, associate professor

Department of biology

Марія Федорівна Іваш, Institute of Cell Biology NAS of Ukraine Drahomanov Str. 14/16, Lviv, Ukraine, 79005

Department of Analytical Biotechnology, engineer

Михайло Васильович Гончар, Institute of Cell Biology NAS of Ukraine, 14/16 Drahomanov str., Lviv, Ukraine, 79005

Professor, Doctor of Biological Science,

The Head of the Department of Analytical Biotechnology 

References

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Published

2015-08-22

Issue

Vol. 8 No. 1(13) (2015)

Section

Biological sciences

License

Copyright (c) 2015 Галина Зуфарівна Гайда, Марія Миколаївна Синенька, Галина Миколаївна Клепач, Марія Федорівна Іваш, Михайло Васильович Гончар

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