Role of systemic antiinflammatory response markers in the pathogenesis of chronic limb ischemia
DOI:
https://doi.org/10.15587/2313-8416.2015.56807Keywords:
cytokines, chronic lower limb ishemia, systemic inflammatory response markers, HIF-1, atherosclerosis mutual burdening syndrome, PAD, duplex scanning, ABIAbstract
Objective: To study the dynamics of systemic inflammatory response markers and protein HIF-1 transcription in experimental model of critical CLLI, to study the content dynamics of intracellular carbohydrate substrates.
Materials and methods: The main clinical group consisted of 50 patients with no signs of diabetes aged 56 to 75 years, mostly men with CCLLI and the comparison group: 25 healthy volunteers aged 45–65 years. Duplex scanning in B-mode of lower limb arteries with the ABI measure was conducted by 4–8 MHz probe. Characteristics of laboratory animals: 40 Chinchilla rabbits with an average weight of 2,5±0,5 kg; aged 15±3 months. Subgroups: I – ischemic hind limbs model by designed device and II – control 10 animals. Lower limbs tissues microcirculation was studied by the laser Doppler flowmetry method in animal models and in patients with arterial occlusion in big toe area. Histological study was performed by MSB method. Biochemical studies: levels of chemo-and cytokinesby ELISA, the PPPW markers activity measurement were done by spectrophotometric methods.
Results: The dynamics of HIF-1 protein in the cytoplasm of muscle cells in vivo and in vitro demonstrated that hypoxia in progressing CCLLI was more severe in vivo due to syndrome of "mutual burden" between the ischemic limb and target organs.
Conclusion: The research allowed to clarify the obscure links in CCLLI pathogenesis, better reflecting its three phases, connecting with HIF-1 synthesis, and high-level chemokine and cytokines in patients suggest an important role of local and systemic inflammatory response
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Copyright (c) 2015 Олена Ігорівна Кефелі-Яновська, Наталія Юріївна Літвінова, Віктор Анатолійович Черняк, Володимир Григорович Мішалов, Ельвіра Айдинівна Джалілова, Дмитро Дубенко, Тарас Григорович Шевченко
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