LC-MS chromatographic fingerprinting as a tool for medicinal plant standardization (Hypericum perforatum L. as a case study)

Taras Maiula — Fri, 29 May 2026 00:00:00 +0300

Aim. To evaluate the applicability of LC-MS chromatographic fingerprinting as a tool for standardization and authenticity verification of medicinal plant material using Hypericum perforatum L. as a model object.

Materials and methods. 50% and 80% hydroethanol extracts of dried aerial parts of H. perforatum were prepared by ultrasonic extraction (30 min, sample weights 504 mg and 516 mg). Analysis was performed by LC-MS on an Agilent 1260 Infinity II / LC/MSD iQ system; Gemini NX-C18 column (150 × 4.6 mm, 5 μm), gradient elution (0.1% formic acid\acetonitrile), flow rate 0.7 mL/min. Detection was carried out in ESI+ and ESI− modes over the m/z range 100–1000. Total ion current (TIC) chromatograms were recorded and compared by overlay profiling.

Results. 12 characteristic peaks were identified in both extracts: phenolic acids (chlorogenic, neochlorogenic, caffeic), flavonoid glycosides (rutin, hyperoside, isoquercitrin), flavan-3-ols (catechin, epicatechin), and quercetin aglycone. Identification was performed at MSI level 2 (tentative annotation). TIC profiles were qualitatively similar (phytoequivalence), with the 80% extract yielding higher signal intensities for key marker compounds.

Conclusions. LC-MS fingerprint analysis provides a reproducible chemical profile suitable as a reference standard for batch verification and detection of compositional deviations. The 80% hydroethanol extract is recommended as the optimal system for generating a reference fingerprint of H. Perforatum

Influence of medicinal products of various pharmacological groups on hemostasis system indicators

Rymma Yeromenko, Maryna Ostapets, Olena Dolzhykova, Igor Seniuk — Fri, 29 May 2026 00:00:00 +0300

Objective. To evaluate the effect of drugs from different pharmacological groups on the hemostasis and fibrinolysis systems and to determine the most significant laboratory changes during pharmacotherapy.

Materials and methods. The study was conducted at a municipal clinical hospital and included 44 patients aged over 52 years. Patients were divided into three groups depending on the therapy received: cardiovascular drugs, antibiotics, and drugs for musculoskeletal disorders. Parameters of platelet, coagulation hemostasis, and fibrinolysis were assessed using a hemocoagulograph.

Results. All studied drug groups affected the platelet component of hemostasis, leading to decreased platelet aggregation and shortened clotting time. The most pronounced changes in coagulation parameters were observed in patients receiving cardiovascular drugs, including reduced activated partial thromboplastin time and prothrombin time. After discontinuation, partial normalization of parameters was observed. The impact on fibrinolysis was minimal in most groups; however, long-term use of cardiovascular drugs showed a tendency toward hypercoagulation, confirmed by increased D-dimer and antithrombin III levels.

Conclusions. Drugs from various pharmacological groups significantly affect hemostasis, primarily the platelet component, while cardiovascular drugs also influence coagulation and fibrinolysis. These findings highlight the importance of considering drug effects when interpreting laboratory results to avoid diagnostic errors

ScienceRise: Biological Science

LC-MS chromatographic fingerprinting as a tool for medicinal plant standardization (Hypericum perforatum L. as a case study)

Influence of medicinal products of various pharmacological groups on hemostasis system indicators