DOI: https://doi.org/10.26641/2307-0404.2017.2.109832

Study of biological action of aerococcus аutosymbionts on the model of staphylococcal infection.

D. O. Stepanskyi, G. M. Kremenchutskyi, I. P. Koshova

Abstract


The paper presents data on the study of the antagonistic effect of aerococcus autosymbionts in experimental models of infections caused by staphylococci. To study the antagonistic action of aerococcus autosymbiont on staphylococcus, a model of chronic staphylococcal infection in white mice and rabbits was used. In staphylococcal experimental infection, aerococcus antagonistic action against staphylococcus was tested by subcutaneous injection on white mice. Aerococci survival under the skin was studied. Aerococcus autosymbionts introduced under the skin to staphylococcus in 5 hours and 3 hours after administration of the latter cause antagonisteffect and inhibit the development of infiltrates. Similar results were obtained when introducing aerococcus autosymbiontsin the focus of infection in 5 minutes, 5 minutes and 3 hours, 1 and 5 hours after infection. Aerococci introduced after 24 and 48 hours shortened terms of disease manifestations as compared to the control group of mice. The therapeutic effect of aerococcus  autosymbiontswas tested on the experimental model of burns and wounds infected with staphylococcus. In animals with wounds treated with aerococcus autostrains the number of pathogenic staphylococci was 10 times less than in the control group. According to our observations aerococcus autosymbionts showed no irritant effect when applied on the wound surface, helped its healing, sharply reduced the percentage of staphylococcus inoculation from wound secretions.


Keywords


aerococcus; antagonistic action; model of staphylococcus infection

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References


1. Stepanskiy DA, Kanevskiy DO, Kheylik OG, Kremenchutskiy GN, Yurgel' LG, Krushinskaya TYu, Sharun OV, Koshevaya IP. [Antagonistic activity of the aerobacterial symbionts in relation to opportunistic and pathogenic microorganisms]. Biomedical and biosocial anthropology. 2012;18:71-73. Russian.

2. Brusina EB. [Immediate tasks of hospital epi­demiology. Strategy and tactics of fighting nosocomial infections at the present stage of medical development: materials of the International Congress]. Мoskva, 2006;43-44. Russian.

3. Brusina EB. [Evolution of the epidemic process of hospital purulent-septic infections in surgery]. Epi­demiologiya i infektsionnye bolezni, 2001;2:10-12. Russian.

4. Abaev JuK, Gudkova EI, Adarchenko AA, Las­tochkina TM. [Pathogens of surgical infection in children: resistance to antiseptics and its dynamics]. Detskaya khirurgiya, 2006;3:30-33. Russian.

5. Menshikov DD, Evdokimova NV, Grunenko­va IV. [Dynamics of antibiotic resistance of pathogens of purulent-septic processes in an ambulance station]. Anti­biotiki i khimioterapiya, 2002;47(8):12-15. Russian.

6. Elik SD. Experimental Staphylococcal infection in the skin of mise. Ann. New–york ac. Sci. 1956;72:96-101.


GOST Style Citations


  1. Антагонистическая активность аэрококков сим­бионтов по отношению к условно-патогенным и па­тогенным микроорганизмам / Д.А. Степанский, Д.О. Ка­невский, О.Г. Хейлик, Г.Н. Кременчуцкий [и др.] // Bio­medical Biosocial Anthropology.– 2012. – № 18. – С. 71-73.
  2. Брусина Е.Б. Неотложные задачи госпиталь­ной эпидемиологии / Е.Б. Брусина // Стратегия и тактика борьбы с внутрибольничными инфекциями на современном этапе развития медицины: материалы междунар. конгресса. – Москва, 2006. – С. 43–44.
  3. Брусина Е.Б. Эволюция эпидемического про­цесса госпитальных гнойно-септических инфекций в хирургии / Е.Б. Брусина // Эпидемиология и инфек­ционные болезни, – 2001. – № 2. – С. 10–12.
  4. Возбудители хирургической инфекции у детей: устойчивость к антисептикам и ее динамика / Ю.К. Абаев, Е.И. Гудкова, А.А. Адарченко, Т.М. Ласточкина // Детская хирургия. – 2006. – № 3. – С. 30–33.
  5. Меншиков Д.Д. Динамика антибиотикоре­зис­тентности возбудителей гнойно-септических процес­сов в стационаре скорой помощи / Д.Д. Меньшиков, Н.В. Евдокимова, И.В. Груненкова // Антибиотики и химиотерапия. – 2002. – № 8, Т. 47. – С. 12–15.
  6. Elik S.D. Experimental Staphylococcal infection in the skin of mise / S.D. Elik // Ann. New–York ac. Sci. – 1956. – Vol. 72. – P. 96-101.