Study of modification of zinc status of laboratory animals in conditions of low-dose action of lead and zinc in various forms.

E. M. Biletska, O. P. Shtepa, V. V. Kalinicheva, S. I. Valchuk

Abstract


In recent years the steady trend to growth of the technogenic load of the environment closely correlates with deterioration of the health status of the population, chronic diseases and worsening of demographic indicators. One of the main threats to human health from contamination with heavy metals is associated with the influence of lead, which contributes to the development of chronic diseases of the nervous, hematopoietic, endocrine, renal and bone systems. In the process of studying the properties of zinc, its use as an osteoprotective agent is becoming more actual. In connection with the foregoing, the aim of our study was to study the content of zinc in bone tissue in low-dose isolated and combined effects of various forms of lead and zinc under the experimental conditions. For the experiment, sexually mature Wistar rats with a body weight of 150-170 g were selected, with the following randomization into six experimental and one control group, eight rats in each one. The data obtained and their comprehensive analysis allow to fully suggest the formation of a bioantagonistic relationship between lead and zinc in the bone at the level of the latter, observed both in the isolated action of lead and in combination with zinc. Along with this, our results correspond with the data of other researchers on the osteoprotective properties of zinc under the action of even low-dose levels of lead, especially of its nanoacqualate form (zinc citrate).


Keywords


lead; zinc; elements; bone

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References


1. Beletskaya EN, Golovkova TA, Onul NM. [Bio­prophylaxis of eco-dependent conditions in the population of industrially developed territories]. Aktual'nye prob­lemy transportnoy meditsiny. 2011;3(2):48-56. Russian.

2. Beletskaya EN, Onul NM, Kalіnіcheva VV. [Comparative evaluation of bioprotective action of zinc in organic and inorganic form on lead osteotropy under experimental conditions]. Medicnі perspektivi. 2016;21(4):123-9. Ukrainian. doi: https://doi.org/­10.26641/2307-0404.2016.4.91481.

3. Pіshak VV, Visots'ka VG, Magalyas VM. [Labo­ratory animals in medico-biological experiments]. Che­rnіvtsі: Med. Universitet. 2006;350. Ukrainian.

4. Antonovich EA, Kagan YuS, Spynu EI. [Gui­delines for the hygienic assessment of new pesticides]. Ministerstvo zdravookhraneniya SSSR; VNII gigieny i toksikologii pestitsidov, polimerov i plasticheskikh mass. Kyiv; 1988. Russian.

5. Teplaya GA. [Heavy metals as a factor of en­vironmental pollution (literature review)]. Astrakhanskiy vestnik ekologicheskogo obrazovaniya. 2013;1(23):182-92. Russian.

6. Gulіch MP, Yemchenko NL, Tomashevs'ka LA. [Iron citrates obtained by aquatic technology: chemical and biological characteristics (evaluation of chemical purity and bioavailability)]. Dovkіllya ta zdorovya. 2011;4(59):11-15. Ukrainian.

7. Jbahi Samira, Monji Saoudi, Kabir Abdelmajid, Oudadesse Hassane, Rebai Treq, Efeki Hafed, Elfeki Abdelfatteh, Keskes Hassib. Accelerated bone ingrowth by local delivery of Zinc from bioactive glass: oxidative stress status, mechanical property, and microarchitectural characterization in an ovariectomized rat model. Libyan Journal of Medicine. 2015;10(1). doi: 10.3402/­ljm.v10.285724.

8. Warheit DB, Laurence BR, Reed KL. Compa­ra­tive pulmonary assessment of single–wall carbon nano­tubes in rats. Toxicol. Science. 2010;77:117-25.

9. Roschger A, Hofstaetter JG, Pemmer B. Diffe­rential accumulation of lead and zinc in double-tidemarks of articular cartilage. Osteoarthritis and Cartilage. 2013;21(11):1707-15. doi: http://dx.doi.org/10.1016/j.jo­ca.2013.06.029.

10. Cantoral A, Téllez-Rojo MM, Shamah Levy T. Differential association of lead on length by zinc status in two-year old Mexican children. Environ Health; 2015. doi: 10.1186/s12940-015-0086-8.

11. Guiping Yuan, Hongke Lu, Zhongqiong Yin, Shujun Dai, Renyong Jia, Jiao Xu, Xu Song, and Li Li. Effects of mixed subchronic lead acetate and cadmium chloride on bone metabolism in rats. Int J Clin Exp Med. 2014;7(5):1378-85.

12. European convention for the protection of verteb­rate animals used for experimental and other scientific purposes. Council of Europe. Strasburg. 1986;53.

13. Mirjam Rauwolf, Bernhard Pemmer, Andreas Roschger, Anna Turyanskaya, Stephan Smolek, Angelika Maderitsch, Peter Hischenhuber, Martin Foelser, Rolf Simon, Susanna Lang, Stephan E Puchner, Reinhard Windhager, Klaus Klaushofer, Peter Wobrauschek, Jochen G Hofstaetter, Paul Roschger, Christina Streli Xray Spectrom. Increased zinc accumulation in mine­ralized osteosarcoma tissue measured by confocal synchrotron radiation micro X‐ray fluorescence analysis. 2017;46(1):56-62. doi: 10.1002/xrs.2727.

14. Kawade R. Zinc status and its association with the health of adolescents: a review of studies in India. Glob Health Action. 2012;5:7353.

15. Kupraszewicz E, Brzóska MM. Excessive ethanol consumption under exposure to lead intensifies disorders in bone metabolism: A study in a rat model. Chem Biol Interact. 2013;203:486-501.

16. Sun Y, Sun D, Zhou Z, Zhu G, Zhang H, Chang X. Osteoporosis in a Chinese population due to occu­pa­tional exposure to lead. Am J Ind Med. 2008;51:436-42.

17. Ema M, Kobayashi N, Naya M. Reproductive and developmental toxicity studies of manufactured na­nomaterials. Reprod. Toxicol. 2010;30:343-52.

18. Pemmer B, Roschger A, Wastl A. Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue. Bone. 2013;57(1):184-93. doi: 10.1016/j.bone.2013.07.038.

19. Nelson AE, Shi XA, Schwartz TA, Chen JC, Renner JB, Caldwell KL. Whole blood lead levels are associated with radiographic and symptomatic knee osteo­arthritis: a cross-sectional analysis in the Johnston County Osteoarthritis Project. Arthritis Res Ther. 2011;13:37.


GOST Style Citations


  1. Белецкая Э.Н. Биопрофилактика экозави­си­мых состояний у населения индустриально развитых тер­риторий / Э.Н. Белецкая, Т.А. Головкова, Н.М. Онул // Актуальные проблемы транспортной медицины.- 2011.- Т.3, №2.- С. 48-56.
  2. Білецька Е.М. Порівняльна оцінка біопротек­торної дії цинку в органічній та неорганічній формі на остеотропність свинцю в експериментальних умовах / Е.М. Білецька, Н.М. Онул, В.В. Калінічева // Медичні перспективи. – 2016. – Т. 21, № 4. – С. 123-129. doi: https://doi.org/10.26641/2307-0404.2016.4.91481
  3. Лабораторні тварини в медико –біологічних експериментах / В.В. Пішак, В.Г. Висоцька, В.М. Ма­галяс [та ін.].- Чернівці: Мед. ун-т, 2006.- 350с.
  4. Методические указания по гигиенической оценке новых пестицидов / Е.А. Антонович, Ю.С. Каган, Е.И. Спыну [и др.] // Министерство здравоохранения СССР; ВНИИ гигиены и токсикологии пестицидов, полимеров и пластических масс. - Киев, 1988.
  5. Теплая Г.А. Тяжелые металлы как фактор за­грязнения окружающей среды (обзор литературы) / Г.А. Теплая // Астрахан. вестник экологического об­разования. – 2013. – Т. 23, № 1. – С. 182-192.
  6. Цитрати заліза, отримані за аквана­нотехноло­гією: хімічна та біологічна характеристика (оцінка хімічної чистоти та біодоступності) / М.П.Гуліч, Н.Л.Єм­ченко, Л.А. Томашевська [та ін.] // Довкілля та здоровя. – 2011. –Т. 59, № 4 – С. 11-15.
  7. Accelerated bone ingrowth by local delivery of Zinc from bioactive glass: oxidative stress status, me­chanical property, and microarchitectural characterization in an ovariectomized rat model / Jbahi Samira , Monji Saoudi , Kabir Abdelmajid [et al.] // Libyan J. Medicine. – 2015. – Vol. 10, Iss. 1. doi: 10.3402/ljm.v10.28572.
  8. Comparative pulmonary assessment of single–wall carbon nanotubes in rats / D.B. Warheit, B.R. Lau­rence, K.L. Reed [et al.] // Toxicol. Science. – 2010. – Vol. 77. – Р. 117-125.
  9. Differential accumulation of lead and zinc in double-tidemarks of articular cartilage / A. Roschger, J.G. Hofstaetter, B. Pemmer [et al.] // Osteoarthritis Carilage. – 2013. – Vol. 21, N 11. – P. 1707-1715. doi: http://dx.doi.org/10.1016/j.joca.2013.06.029.
  10. Differential association of lead on length by zinc status in two-year old Mexican children / A. Cantoral, M.M. Téllez-Rojo, T. Shamah Levy [et al.] // Environ Health. – 2015. doi: 10.1186/s12940-015-0086-8.
  11. Effects of mixed subchronic lead acetate and cadmium chloride on bone metabolism in rats / Guiping Yuan, Hongke LuZhongqiong Yin [et al.] // Int. J. Clin. Exp. Med. – 2014. – Vol. 7, N 5. – P. 1378-1385. 
  12. European convention for the protection of ver­tebrate animals used for experimental and other scientific purposes. Council of Europe.- Strasburg, 1986.- 53 p.
  13. Increased zinc accumulation in mineralized osteo­sarcoma tissue measured by confocal synchrotron ra­diation micro X‐ray fluorescence analysis / M. Rau­wolf, B. Pemmer, A. Roschger [et al.] // Xray Spectrom. – 2017. – Vol. 46, N 1. – P. 56-62. doi: 10.1002/xrs.2727.
  14. Kawade R. Zinc status and its association with the health of adolescents: a review of studies inIndia/ R. Kawade / Glob. Health. Action. – 2012. – Vol. 5. –P. 7353.
  15. Kupraszewicz E. Excessive ethanol consumption under exposure to lead intensifies disorders in bone me­tabolism: A study in a rat model /E. Kupraszewicz, M.M. Brzóska // Chem. Biol. Interact. – 2013. – Vol. 203. – P. 486-501.
  16. Osteoporosis in a Chinese population due to occu­pational exposure to lead / Y. Sun, D. Sun, Z. Zhou [et al.] // Am. J. Ind. Med. – 2008. – Vol. 51. – P. 436-442. 
  17. Reproductive and developmental toxicity studies of manufactured nanomaterials / M. Ema, N. Kobayashi, M. Naya [et al.] // Reprod. Toxicol. – 2010. – Vol. 30. – P. 343-352.
  18. Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue / B. Pemmer, A. Roschger, A. Wastl [et al.] // Bone. – 2013. – Vol. 57, N 1. – P. 184-193. doi: 10.1016/j.bone.2013.07.038.
  19. Whole blood lead levels are associated with radiographic and symptomatic knee osteoarthritis: a cross-sectional analysis in the Johnston County Osteoarthritis Project / A.E. Nelson, X.A. Shi, T.A. Schwartz. [et al.] // Arthritis Res. Ther. – 2011. –Vol. 13. – P. 37.




DOI: https://doi.org/10.26641/2307-0404.2017.4.117661

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