The role of dendritic cells the in development of atherosclerosis of coronary vessels in the experiment.

Authors

  • N. S. Tryasak

DOI:

https://doi.org/10.26641/2307-0404.2018.1.124903

Keywords:

myocardium, atherosclerosis, dendritic cells, S-100

Abstract

The dynamics of quantitative and qualitative changes of the dendritic cells (DC) of coronary arteries wall at different stages of atherosclerosis development was investigated. Animals received human native low density lipoproteins. Dendritic cells were detected by using polyclonal antibodies S-100 and monoclonal antibodies CD1a (clone O10). A semiquantitative scale from 0 to 3 points was used to determine the intensity of expression of immunohistochemical markers. Histological examination of coronary arteries wall was perfomed by hemothoxylin-eosin, orsein andsudanIII. The first increase of the number of dendritic cells was observed at the 10th week after start of experiment, which corresponded to prelipid stage of atherosclerosis. The second increase of the number of dendritic cells was noted at the 13week and was characterized by moderate expression of markers (2 points) and morphologically responded to the stage of lipoidosis. The late stages of the experiment (18-20 week) were characterized by the maximal increase of DC and a high expression of immunohistochemical markers (3 points).

Author Biography

N. S. Tryasak

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Department of pathophysiology
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

References

[European convention for the protection of vertebrate animals used for experimental and other scien­tific purpose: Council of Europe 18.03.1986].Strasbourg. 1986;52. Ukrainian.

Zapadnyuk IP. [Laboratory animals. Breeding, maintenance, use in the experiment]. Kyiv, Visha shkola. 1983;383. Russian.

Menshykov IV. [Experimental model of athero­sclerosis in rats caused by immunization with native human lipoproteins]. Vestnik Udmurtskogo universiteta. 2012;1:80-86. Russian.

[Law ofUkraine. “On Protection of Animals from Cruel Treatment”, 21.02.2006 N 3447-IV]. Vіdomostі Verhovnoї Radi Ukraїni (VVR). 2006;27:230. Ukrainian.

Rebrova OYu. [Statistical analysis of medical data. Application of the Statistica software package]. Moskva, Mediаsfera. 2006;312. Russian.

Karpov AM. [The role of dendritic cells in the pathogenesis of atherosclerosis]. Doktor.ru. 2015;8-9(109-110):4-8. Russian.

Barchet W, Cella M, Colonna M. Plasmacytoid dendritic cells - virus eperts of innate immunity. Semin Im­munol. 2005;17(4):253-61.

Bobryshev Y.V. Dendritic cells in atherosclerosis: current status of the problem and clinical relevance. Eur. Heart J. 2005;26(17):1700-04.

Bobryshev YV, Lord RS. S-100 positive cells in human arterial intima and in atherosclerotic lesions. Car­diovasc Res. 1995;29(5):689-96.

Cybulsky MI, Cheong C, Robbins CS. Macro­phages and Dendritic Cells: Partners in Atherogenesis. Circ Res. 2016;118(4):637-52.

Döring Y, Zernecke A. Plasmacytoid dendritic cells in atherosclerosis. Front Physiol. 2012;28(3):230-37.

Alderman CJ, Bunyard PR, Chain BM, Foreman JC, et al. Effects of oxidised low density lipoprotein on dendritic cells: a possible immunoregulatory component of the atherogenic micro-environment. Cardiovasc Res. 2002;55(4):806-19.

Galkina E, Ley K. Immune and inflammatory mecha­nisms of atherosclerosis. Annu Rev Immunol. 2009;27:165-97.

Kassiteridi C, Monaco C. Macrophages and den­dritic cells: the usual suspects in atherogenesis. Curr Drug Targets. 2015;16(4):373-82.

Kumar GL, Rudbeck L. [Immunohistochemical methods:]. DAKO Manual, editor GA Franko, PG Malkova, Moskva. 2011;224. Russian.

Chistiakov DA,SobeninIA, Orekhov AN, et al. Myeloid dendritic cells: Development, functions, and role in atherosclerotic inflammation. Immunobiology. 2015;220(6):833-44.

Idoyaga J, Fiorese C, Zbytnuik L, et al. Spe­cialized role of migratory dendritic cells in peripheral tolerance induction. J Clin Invest. 2013;123(2):844-54.

Yamane H, Paul WE. Early signaling events that un­derlie fate decisions of naive CD4(+) T cells toward dis­tinct T-helper cell subsets. Immunol Rev. 2013;252(1):12-23.

Downloads

How to Cite

1.
Tryasak NS. The role of dendritic cells the in development of atherosclerosis of coronary vessels in the experiment. Med. perspekt. [Internet]. 2018Apr.2 [cited 2024Apr.25];23(1):4-8. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/124903

Issue

Vol. 23 No. 1 (2018)

Section

THEORETICAL MEDICINE

License

Copyright (c) 2018 Medicni perspektivi

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Submitting manuscript to the journal "Medicni perspektivi" the author(s) agree with transferring copyright from the author(s) to publisher (including photos, figures, tables, etc.) editor, reproducing materials of the manuscript in the journal, Internet, translation into other languages, export and import of the issue with the author’s article, spreading without limitation of their period of validity both on the territory of Ukraine and other countries. This and other mutual duties of the author and all co-authors separately and editorial board are secured by written agreement by special form to use the article, the sample of which is presented on the site.

Author signs a written agreement and sends it to Editorial Board simultaneously with submission of the manuscript.