Monitoring of Enterobacteria strains with producing β-lactamases in males with infectious-inflammatory diseases of urogenital tract
DOI:
https://doi.org/10.26641/2307-0404.2022.2.260282Keywords:
opportunistic pathogenic microflora, urogenital tract, enterobacteria, β-lactamases, antibiotics, resistanceAbstract
This article presents the findings of investigation of the microflora of 257 males with infectious-inflammatory diseases of the urogenital tract using the test system “Androflor”. The role of representatives of conditionally pathogenic microflora as the main etiological agent in occurrence of infectious-inflammatory diseases of the urogenital tract in males was shown. Its composition in 39.3±3.0% of cases was represented by bacteria of family Enterobacteriaceae, in 10.9±1.9% – by Enterococcus spp., in 3.1±1.1% – by Haemophilus spp. and in 0.4±0.1% of cases – by P. aeruginosa. Out of 101 strains of enterobacteria, 27 representatives had the ability to synthesize β-lactamases 26.7±4.4% of cultures), in particular: 16 isolates of E. coli, 5 isolates of P. mirabilis and 6 isolates of K. pneumoniae. The selected strains of enterobacteria producing β-lactamases appeared to be resistant at least to 6 tested antibiotics, which allowed referring them to categories of multi-resistant. All cultures producing β-lactamases showed resistance to penicillinеs – ampicillin and amoxiclav. In this case, resistance to at least one of the antibiotics of cephalosporin group of the third generation was noted. In addition, 83.3±2.1% of the examined isolates were characterized by resistance to macrolides – erythromycin and azithromycin, as well as to co-trimoxazole and fosfomicin. The obtained data are of practical importance to develop efficient schemes of antibiotic therapy for infectious-inflammatory diseases of the urogenital tract, caused by strains of enterobacteria producing β-lactamases.
References
Antomonov MYu. [Mathematical processing and analysis of medical and biological data]. Kyiv; 2017. p. 579. Russian. Available from: http://www.health.gov.ua/www.nsf/16a436f1b0cca21ec22571b300253d46/522e94120f630ce5c225803b004b3867/$FILE/Antomonov_monogr_titul%2BOGLAVL%2Bvvedenie.pdf
Aminul P, Anwar S, Molla M, Miah R. Evaluation of antibiotic resistance patterns in clinical isolates of Klebsiella pneumoniae in Bangladesh. Biosafety and Health. 2021;3(6):301-6. doi: https://doi.org/10.1016/j.bsheal.2021.11.001
Ayelign B, Abebe B, Shibeshi A, Meshesha S, Shibabaw T, Addis Z, et al. Bacterial isolates and their antimicrobial susceptibility patterns among pediatric patients with urinary tract infections. Turk J Urol. 2018;44(1):62-69. doi: https://doi.org/10.5152/tud.2017.33678
Ben Ashur A, El Magrahi H, Elkammoshi A, Al-sharif H. Prevalence and antibiotics susceptibility pattern of urine bacterial isolates from Tripoli Medical Center (TMC), Tripoli, Libya. Iberoam J Med. 2021;3(3):221-6. doi: https://doi.org/10.53986/ibjm.2021.0035
Caron Y, Chheang R, Puthea N, Soda M, Boyer S, Tarantola A, Kerleguer A. Beta-lactam resistance among Enterobacteriaceae in Cambodia: The four-year itch. Int J Infect Dis. 2018;66:74-79. doi: https://doi.org/10.1016/j.ijid.2017.10.025
Bush K, Bradford PA. Epidemiology of β-lacta-mase-producing pathogens. Clin Microbiol Rev. 2020;33(2):e00047-19. doi: https://doi.org/10.1128/CMR.00047-19
Madrazo M, Esparcia A, Lopez-Cruz I, Albe-rola J, Piles L, Viana A, et al. Clinical impact of multidrug-resistant bacteria in older hospitalized patients with community-acquired urinary tract infection. BMC Infect Dis. 2021;21(1):1232. doi: https://doi.org/10.1186/s12879-021-06939-2
CLSI. Methods for dilution antimicrobial suscep-tibility tests for bacteria that grow aerobically. 11th ed. CLSI standart M07. Wayne, PA: Clinical and Laboratory Standarts Institute; 2018;13. Аvailable from: https://clsi.org/media/1928/m07ed11_sample.pdf
CLSI. Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2020;332. Аvailable from: https://www.nih.org.pk/wp-content/uploads/2021/02/CLSI-2020.pdf
Rajivgandhi G, Maruthupandy M, Ramachan-dran G, Priyanga M, Manoharan N. Detection of ESBL genes from ciprofloxacin resistant Gram negative bacteria isolated from urinary tract infections (UTIs). Front Lab Med. 2018;2(1):5-13. doi: https://doi.org/10.1016/j.flm.2018.01.001
Anesi JA, Lautenbach E, Nachamkin I, Gar-rigan C, Bilker WB, Omorogbe J, et al. Poor clinical out-comes associated with community-onset urinary tract infections due to extended-spectrum cephalosporin-resis-tant Enterobacteriaceae. Infect Control Hosp Epidemiol. 2018;39(12):1431-5. doi: https://doi.org/10.1017/ice.2018.254
Siles-Guerrero V, Cardona-Benavedes I, Liebana-Martos C, Vasquez-Alonso F, Exposito-Ruiz M, Navarro-Mari JM, Gutierrez-Fernandez J. Recent clininical rele-vance of mono-genital colonization/infection by Urea-plasma parvum. Eur J Clin Microbiol Infect Dis. 2020;39:1899-905. doi: https://doi.org/10.1007/s10096-020-03928-2
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Medicni perspektivi
This work is licensed under a Creative Commons Attribution 4.0 International License.
Submitting manuscript to the journal "Medicni perspektivi" the author(s) agree with transferring copyright from the author(s) to publisher (including photos, figures, tables, etc.) editor, reproducing materials of the manuscript in the journal, Internet, translation into other languages, export and import of the issue with the author’s article, spreading without limitation of their period of validity both on the territory of Ukraine and other countries. This and other mutual duties of the author and all co-authors separately and editorial board are secured by written agreement by special form to use the article, the sample of which is presented on the site.
Author signs a written agreement and sends it to Editorial Board simultaneously with submission of the manuscript.
