Comparative assessment of the effect of titanium dioxide – based nanoparticles on boar germ cells in vitro




nanoparticles of titanium dioxide, nanotoxicology, succinate dehydrogenase, cytochrome oxidase, boar germ cells, nanocomposite of titanium dioxide with silver


Modern titanium dioxide nanomaterials, in particular modified with nanosilver, have antiviral, antibacterial, antifungal activity, cytotoxic effects in vitro experiments and can affect germ cells of warm-blooded laboratory animals. The research aims to study the effect of different types of titanium dioxide nanoparticles on the physiological and biochemical characteristics of wild boar germ cells in vitro. When applying the maximum dose (LD50) of the studied nanopowders in all samples, sperm survival was probably lower than in the control: by 41.9% (р<0.001) ‒ under the action of TiO2 nanoparticles (hereinafter NPs) (Frantsevich Institute for Problems of Materials Science of NAS of Ukraine, hereinafter IPM), by 28.0% (р<0.05) ‒ under the action of TiO2 NPs (Acros Organics), by 53.5% (р<0.001) ‒ under the action of 4% Ag-TiO2 NPs (IPM) and by 55.9% (р<0.001) ‒ are the action of NPs 8% Ag-TiO2 (IPM). Nanopowders of different based on titanium dioxide manufacturers, characterized by similar morphological characteristics and varying degrees of nanosilver addition (Ag content from 0 to 8%), starting from a dose of 1/10 LD50, can have a damaging effect on the germ cells of wild boars, which is manifested by a decrease in the respiratory activity of sperm and cytochrome oxidase activity, by increasing succinate dehydrogenase activity. The most sensitive marker was the sperm survival rate, the value of which significantly decreased under the action of 8% Ag-TiO2 nanoparticles (IPM) at a dose of 1/100 LD50, which is a consequence of disruption of the activity of mitochondrial enzymes and, accordingly, resynthesis of ATP. Our results confirm and extend the data on the nature of the damaging effect of titanium dioxide-based nanoparticles on germ cells of warm-blooded animals due to oxidative stress. The obtained experimental data will be taken into account in the hygienic regulation of the investigated nanopowders.


Waghmode MS, Gunjal AB, Mulla JA, et al. Stu-dies on the titanium dioxide nanoparticles: biosynthesis, applications and remediation. SN Appl. Sci. 2019;1:310. doi:

Zahornyi M, Tyschenko N, Shyrokov O, et al. The Effect of Ag Content on the Structural, Optical, and Cytotoxicity Properties of TiO2 Nanopowders Grown from TiO(OH)2 Precursor by the Chemical Deposition Method. Nanosistemi, Nanomateriali, Nanotehnologii. 2021;19(4):923-40. doi:

Zahornyi MM, Yavorovsky OP, Riabovol VM, et al. [Morphological, spectral and toxicological features of new composite material of titanium nanodioxide with nanosilver for use in medicine and biology]. Medicni perspektivi. 2022;27(1):152-9. Ukrainian. doi:

Pankivska YuB, Biliavska LO, Povnitsa OYu, et al. [Antiadenoviral activity of titanium dioxide nano-particles]. Mikrobiolohichnyi zhurnal. 2019;81(5):73-84. Ukrainian. doi:

Kermani SA, Salari S, Nejad Almani PG. Com-parison of antifungal and cytotoxicity activities of tita-niumdioxide and zinc oxide nanoparticles with ampho-tericin Bagainst different Candida species: In vitro evaluation. J Clin Lab Anal. 2021;35(1):e23577. doi:

de Dicastillo CL, Correa MG, Martínez FB, et al. Antimicrobial Effect of Titanium Dioxide Nanoparticles. In: Mareș M, Lim SHE, Lai K, Cristina R, editors. Anti-microbial Resistance – A One Health Perspective [Inter-net]. London: IntechOpen; 2020 [cited 2022 Jul 12]. doi:

Riabovol VM, Kozar TI. [Investigation of acute toxic effects of nanopowders of titanium dioxide and its composites with nanosilver in laboratory mice. Ecological and hygienic problems of the sphere of human life]. Col-lection of materials of the scientific-practical conference with international participation; Kyiv; 2022. p. 160-2. Ukrainian.

Alaee S, Ilani M. Effect of Titanium Dioxide Na-noparticles on Male and Female Reproductive Systems. Journal of Advanced Medical Sciences and Applied Technologies. 2017;3(1):3-8. doi:

Guerreiro DD, Mbemya GT, Bruno JB, et al. Invitro culture systems as an alternative for female reproductive toxicology studies. Zygote. 2019;27(2):55-63. doi:

Demetska OV, Didenko MM, Movchan VO, et al. [Screening assessment of the damaging effect of nanomaterials using bovine spermatozoa as a test object]. Pivdennoukrainskyi medychnyi naukovyi zhurnal. 2021;29:12-15. Ukrainian.

[Express method for determining the toxicity of nanomaterials in in vitro solutions using bovine spermatozoa as a test object]: pat. 101308 Ukraine. No. u201412531; appl. 19.12.2014; publ. 10.09.2015, bull. No. 17. 2 p. Ukrainian. Available from:

Vlizlo VV, Fedoruk RS, Ratych IB, et al. [Laboratory research methods in biology, animal husbandry and veterinary medicine]. Vlizlo VV, edotir. Lviv: Spolom; 2012. Ukrainian. ISBN 976-966-665-677-6.

Antomonov MYu. [Mathematical processing and analysis of medical and biological data]. Кyiv: Med-inform. 2018. Russian.



How to Cite

Yavorovsky O, Zazuliak T, Ostapiv D, Riabovol V, Demetska O. Comparative assessment of the effect of titanium dioxide – based nanoparticles on boar germ cells in vitro. Med. perspekt. [Internet]. 2022Dec.29 [cited 2023Mar.25];27(4):13-9. Available from: