The influence of hypoxia-hypercapnia on the structural state of cellular membranes of rat hepatocytes

Authors

  • Світлана Володимирівна Хижняк National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine
  • Сергій Дмитрович Мельничук National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine
  • Людмила Іванівна Степанова Taras Shevchenko Kyiv National University 60 Vladimirska str., Kyiv, Ukraine, 01601, Ukraine
  • Світлана Миколаївна Мідик National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.51962

Keywords:

fluorescent probes, mitochondria, membranes, lipid bilayer, microviscosity, hypoxia, hypercapnia

Abstract

The structural and dynamic state of cellular membranes of rat hepatocytes under the influence of hypoxia, hypercapnia and hypothermia factors (artificial hypobiosis) was investigated using the method of fluorescent probes. The diverse changes of the structure and physical properties of these membranes (especially of inner mitochondrial membrane) were shown. The structural reorganization of the membrane surface area, the decrease of the lipid structural orderliness and conformational modification of proteins occur during artificial hypobiosis

Author Biographies

Світлана Володимирівна Хижняк, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Professor, Doctor of biology sciences, leading research worker

Ukrainian laboratory of quality and safety of agricultural products

Сергій Дмитрович Мельничук, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Academician of the Ukrainian academy of agrarian sciences, doctor in biology, professor

Animal biochemistry, production quality and safety department named after academician M. F. Gulyy

Людмила Іванівна Степанова, Taras Shevchenko Kyiv National University 60 Vladimirska str., Kyiv, Ukraine, 01601

Candidate of biology sciences, senior research worker

Laboratory of Physical and Chemical Biology of NSC "Institute of Biology"

Світлана Миколаївна Мідик, National University of Life and Environmental Sciences of Ukraine 15 Heroyiv Oborony str., Kyiv, Ukraine, 03041

Candidate of veterinary sciences, senior research worker

Ukrainian laboratory of quality and safety of agricultural products

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Published

2015-10-29

Issue

Vol. 10 No. 6 (15) (2015)

Section

Biological sciences

License

Copyright (c) 2015 Світлана Володимирівна Хижняк, Сергій Дмитрович Мельничук, Людмила Іванівна Степанова, Світлана Миколаївна Мідик

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