Immunohistochemical expression of intermediate filaments in diagnostics of diffuse gliomas.

Authors

  • T. V. Shynkarenko

DOI:

https://doi.org/10.26641/2307-0404.2017.3.111932

Keywords:

brain tumor, immunohistochemistry, diagnostics, vimentin, GFAP

Abstract

Intermediate filament proteins play an important role in maintaining a stable shape, as well as the possible movement of cells, including neoplastic cells. However there, are few works dedicated to the investigation of these proteins expression in diffuse gliomas and they are quite contradictory. The authors studied the expression of intermediate filaments (glial fibrillar acid protein GFAP, vimentin) in diffuse gliomas of the brain. 52 postoperative samples with the diagnosis of astrocytic and oligodendroglial brain tumors were examined. Primary antibodies against GFAP, vimentin, Ki-67 were used. The proliferative index and the expression area were calculated using the tools ImageJ 1.47v. The statistical Fisher test was included. Most samples with moderate expression of GFAP (62.5%) are rated to oligodendroglial tumors. Partof oligodendroglial tumors (58.3%) showed no expression of vimentin by tumor cells,this was not observed in astrocytomas. Meanwhile, a high level of expression of intermediate filament proteins - GFAP (100%) and vimentin (87%) is characteristic for diffuse gliomas. The expression level of GFAP and vimentin is higher in astrocytic tumors than in oligodendroglial (p<0.05). Levels of GFAP and vimentin expression are differential feature of astrocytic and oligodendroglial tumors.

Author Biography

T. V. Shynkarenko

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

References

1. Antomonov M. [Mathematical processing and ana­lysis of medical and biological data.] Kyiv, FMD. 2006;558. Russian.

2. Poslavska OV. [Methodology for the use of sof­tware for the analysis of digital micrographs on the base of pathomorphology course in order to increase the professional level of students and scientists]. Morpho­logia. 2015;9(3):122-6. Ukrainian.

3. Shpon‘ka IS, Shynkarenko TV, Poslavska OV. [Characteristics and analysis of Ki-67-immunoreactivity in brain astrocytoma]. Morphologia. 2016;10(1):96-101. Ukrainian.

4. Dabbs DJ. Diagnostic immunohistochemistry. Elsevier Health Sciences; 2013.

5. Bai HX, Zou Y, Lee AM, Tang X, Zhang P, Yang L. Does morphological assessment have a role in clas­sifying oligoastrocytoma as' oligodendroglial'versus' astro­cy­tic'?. Histopathology. 2016;68(7):1114-5. doi: 10.1111/his.12891.

6. Lepekhin EA, Eliasson C, Berthold CH, Berezin V, Bock E, Pekny M. Intermediate filaments regulate as­trocyte motility. Journal of neurochemistry. 2001;79(3):617-25. doi: 10.1046/j.1471-4159.2001.00595.x.

7. Kidd ME, Shumaker DK, Ridge KM. The role of vimentin intermediate filaments in the progression of lung cancer. American journal of respiratory cell and mo­lecular biology. 2014;50(1):1-6. doi: 10.1165/rcmb.2013-0314TR.

8. Lépinoux-Chambaud C, Eyer J. Review on in­termediate filaments of the nervous system and their pathological alterations. Histochemistry and cell biology. 2013;140(1):13-22. doi:10.1007/s00418-013-1101-1.

9. Li L, Lundkvist A, Andersson D, Wilhelmsson U, Nagai N, Pardo AC, Nodin C, Ståhlberg A, Aprico K, Larsson K, Yabe T. Protective role of reactive astrocytes in brain ischemia. Journal of Cerebral Blood Flow & Metabolism. 2008;28(3):468-81. doi: 10.1038/sj.jcbfm.9600546.

10. Ikota H, Kinjo S, Yokoo H, Nakazato Y. Sys­tematic immunohistochemical profiling of 378 brain tumors with 37 antibodies using tissue microarray tech­nology. Acta neuropathologica. 2006;111(5):475-482. doi: 10.1007/s00401-006-0060-1.

11. Yachnis AT, Rivera-Zengotita ML. Neuropa­thology, A Volume in the High Yield Pathology Series (Expert Consult-Online and Print), 1: Neuropathology. El­sevier Health Sciences; 2013.

12. Cavenee WK, Leung SY, Hawkins C, Van Meir EG, Burger PC, & Tabori U WHO Classification of Tu­mours of the Central Nervous System, Revised. ISBN 978-92-832-4492-9.

Downloads

How to Cite

1.
Shynkarenko TV. Immunohistochemical expression of intermediate filaments in diagnostics of diffuse gliomas. Med. perspekt. [Internet]. 2017Oct.12 [cited 2024Nov.22];22(3):93-8. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/111932

Issue

Section

CLINICAL MEDICINE