Experimental determination of the accumulation of cadmium salts in the heart of the embryo of rat and their effect on rat cardiogenesis

Authors

DOI:

https://doi.org/10.26641/2307-0404.2020.3.214628

Keywords:

rat embryo, cardiogenesis, cadmium, metal citrate, sulfur citrate, iodine citrate, heart

Abstract

The article discusses the results of an experimental effect of cadmium chloride / cadmium citrate on the cardiogenesis in conditions of intragastric administration to pregnant female rats. The aspect of accumulation of cadmium salts in the heart of the embryo was studied under the condition of daily administration of a salt solution to female rats from the first day of pregnancy to the end of embryogenesis (20 days). In addition to the groups of isolated cadmium administration, there were groups of combined administration of cadmium salts with citrates of germanium, cerium, and the iodine + sulfur composite. The second task was to study the effect of the studied salt solutions on the cardiogenesis of rat’s embryos, for which serial histological sections of the heart were made. The thickness of the compact myocardium of the heart chambers was measured as the main indicator of cardiogenesis. The use of multielement analysis showed that the highest level of cadmium accumulation was found in the group of isolated cadmium citrate administration against the background of a decrease in the zinc content in the heart tissues of the embryo. It has been proven that cadmium citrate maintains a high level of accumulation in the groups with combined administration of cerium citrate, germanium citrate, iodine + sulfur citrate composites, but in these groups the level of zinc in the myocardium significantly increases, which can be regarded as an element of the compensatory effect of cerium citrate, germanium and composite iodine + sulfur.The effect of the indicated doses of cadmium salts on cardiogenesis in rats was reflected in the thickness of the myocardial layer of all chambers of the heart in different ways: cadmium chloride thickened the walls of the left and right ventricles, both atria with a thickening of the interventricular septum. Cadmium citrate provokes thinning of the myocardium of the wall of both ventricles and local thickening of the interventricular septum. In the groups of combined administration, the indicators of the thickness of the compact myocardium restored, which indicates the modifying effect of citrates of the studied trace elements on the cardiotoxicity of cadmium salts.

Author Biographies

E. A. Nefedova

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

A. I. Halperin

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

V. F. Shatornaya

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

I. V. Shevchenko

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

Yu. V. Demidenko

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

I. A. Pridius

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

Yu. P. Myasoyed

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
department of clinical anatomy, anatomy and operative surgery
V. Vernadsky str., 9, Dnipro, 49044, Ukraine

References

Apykhtina OL. [Changes in the biochemical parameters of the blood serum of experimental animals after exposure to cadmium chloride and cadmium sulfide nanoparticles of different sizes]. Biomedical and Bio­social Anthropology. 2017;1:47-51. Russian.

Garets VI, Shatorna VF, Ostrovskaya SS, Kono­nova II, Krasnov OO. [Methodical approaches to determi­nation of embryotoxicity and teratogenicity of heavy metals in morphological experiments[. Ukrainian Journal of Medicine, Biology and Sports. 2015;1(1):189-94. Ukrainian.

Grzhegotsky MR, Sukhodolskaya NV. [The effect of copper, zinc, cadmium and lead on the likelihood of de­veloping a threat of termination of pregnancy in women]. Reproductive health. 2014;1(31):43-49. Russian.

Dinerman AA. The role of environmental pollu­tants in impaired embryonic development. Moskva: Medicine, 1980. р. 191. Russian.

Nefyodovа OO, Yeroshenko GA, Zadesenets IP, Shatorna VF, Nefyodov OO. [Effect of low doses of cad­mium citrate on the cardiogenesis of rat embryos]. The world of medicine and biology. 2019;1(67):166-70. Ukrainian.

Oberlis D, Skalny AV, Skalnaya MG, Nikonoro­va AA. [Pathophysiology of microelementoses]. Pathoge­nesis. 2016;14(2):20-27. Russian.

Skalny AV. [Assessment and correction of the elemental status of the population is a promising area of domestic health care and environmental monitoring]. Microelements in medicine. 2018;19(1):5-13. Russian. doi: https://doi.org/10.19112/2413-6174-2018-19-1-5-13

Shatorna VF, Chekman IS, Garets VI, Nefyodo­va OO, Kaplunenko VG. [Experimental study of the influen­ce of nanometals on embryogenesis and heart development]. Integrative Anthropology. 2017;1:59-63. Ukrainian.

Shafran LM, Pikhtieva OG, Bolshoy DV. [An al­gorithm for laboratory tests for suspected dyshomeostasis of heavy metals]. Actual problems of transport medicine. 2014;1(38-1):97-105.

Chowdhury R, Ramond A, O’Keeffe LM, Shah­zad S, Kunutsor SK, Muka T, Gregson J, Willeit P, War­nakula S, Khan H, Chowdhury S. Environmental toxic metal contaminants and risk of cardiovascular disease: systematic review and meta-analysis. BMJ. 2018;362:k3310.

Lamas GA, Navas-Acien A, Mark DB, Lee KL. Heavy metals, cardiovascular disease, and the unexpected benefits of edetate chelation therapy. J Am Coll. Car­diol. 2016;67:2411-8. doi: https://doi.org/10.1016/j.jacc.2016.02.066

Published

2020-10-05

How to Cite

1.
Nefedova EA, Halperin AI, Shatornaya VF, Shevchenko IV, Demidenko YV, Pridius IA, Myasoyed YP. Experimental determination of the accumulation of cadmium salts in the heart of the embryo of rat and their effect on rat cardiogenesis. Med. perspekt. [Internet]. 2020Oct.5 [cited 2024Oct.14];25(3):8-16. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/214628

Issue

Section

THEORETICAL MEDICINE