Modeling of chronic generalized periodontitis in laboratory animals (literature review)




regenerative dentistry, chronic generalized periodontitis, modeling, laboratory animals


Modern experiments in the study of periodontal deseas are aimed at improving the effectiveness of innovative methods of early diagnosis, treatment and prevention of this disease in patients. The conducted analysis of the literature showed that the disorder of microcirculation occupies one of the leading places in the pathogenesis of periodontitis. Disruption of the microcirculatory bed, based on the dyscirculatory changes of the capillary blood flow is accompanied by a decrease in the intensity of blood filling of vessels, their spasm. These changes, in case of chronization, include tissue disorders of the ischemic nature, up to the development of a capillary stasis. This can lead to disorder in gas exchange, trophism and pathomorphological damage to periodontal tissues. This type of change is most typical for age-related, traumatic, vascular, and stress-induced models. Morphological lesions in the vessels, namely ischemic phenomena were most pronounced in them. Microscopically, this was manifested in the heterogeneity of the epithelial layer, the thickening of individual layers, the detachment of the epithelial lining of the mucosa. As for the basal layer, the most common was the vacuolization of cells, the appearance of a significant number of cells that lacked nuclei (pathology, indicating a decrease in functional activity, impaired regenerative properties of the cell), expansion of intercellular contacts, a sign of tissue edema. In the basal membrane (function - dissociation of the epithelium from the actual lining of the mucous membrane), collagen fibers were loose, hypertrophic, their hyperplasia was observed, neutrophil leukocytes, lymphocytes, histocytes were found between the fibers, this testified to changes in cells. Some models lack a clinical picture of chronic hyperplastic periodontitis. The disorders consisted of changes in normal metabolism in periodontal tissues, decreased immune function, changes in morphological structure without induction of the inflammatory process.

Author Biographies

O. V. Kopchak

Private Higher Education Establishment «Kyiv Medical University»
Boryspilska Str., 2, Kyiv, 02099, Ukraine

N. S. Marchenko

Private Higher Education Establishment «Kyiv Medical University»
Boryspilska Str., 2, Kyiv, 02099, Ukraine

Ya. V. Yanishevska

Private Higher Education Establishment «Kyiv Medical University»
Boryspilska Str., 2, Kyiv, 02099, Ukraine


[European Convention for the Protection of Vertebrate Animals Used for Research and Other Scien­tific Purposes]. Strasbourg; 1986. March 18. Ukrainian. doi:

Nikolayeva АV. [Development of model of ex­perimental periodontitis in rats in terms of action of vitamin K antagonist]; 2011. p. 1-2. Ukrainian.

[Order 01.03.2012 No. 249, dated 16th March, 2012 No. 416/20729 on approval of carrying out ex­periments on animals by scientific establishments]. (2012). Ukrainian.

Rusanov VP, Yermukhanova TG. [The basis of the use of grafts based on autologous multicomponent mesinchymal stromal fat cells in the surgical treatment of experimental periodontitis]; 2015. p. 2-3. Ukrainian.

Demkovych АYe, Bondarenko YuІ. [Patent 82388 Ukraine, IPC 3 09 В 23/28 (2006.01) А 61 В 17/00. Means of modeling periodontitis in laboratory animals (rats) / No. i201303000; appl. 11.03.2013; publ. 25.07.2013, Bulletin No. 14]. (2013). Ukrainian. doi:

Shymansky L, Chilikin VN, Malshev IU, et al. [Phagocytic defense of periodontium and means of its activation]. Dentistry. 2013;92(5):64-69. Russian.

Al-Khabbaz AK, Al-Shammari KF. Diabetes, mel­li­tus and periodontal health: dentists' knowledge. Med. Princ. Pract. 2011;20(6):538-44. doi:

Andrian E, Grenier D, Rouabhia M. Porphyro­mo­nas gingivalis-epithelial cell interactions in periodontitis. J Dent Res. 2006;85:392-403. doi:

Kowalski M, Brocka E, Barylski M, et al. Asses­sment of the periodontal state in subjects with metabolic syndrome. Pol. Merkur. Lekarski. 2009;26(156):620-5.

Charbel Choubaya, Ramez Chahine, Pierre Zal­loua, Ziad Salameh Periodontitis and diabetes interre­lationships in rats: biochemical and histopathological variables. Journal of Diabetes & Metabolic Disorders. June 2019;18:(1):163-72. doi:

Lu H, Xu M, Wang F, Liu S, Gu J, Lin S, Zhao L. Chronic stress accelerates ligature-induced periodontitis by suppressing glucocorticoid receptor-α signaling. Exp Mol Med. 2016 Mar 25;48:e223. doi:

Peruzzo DC, Benatti BB, Antunes IB, et al. Chro­nic stress may modulate periodontal disease: a study in rats. J Periodontol. 2008;79(4):697-704. doi:

Dannan A, Alkattan F. Animal Models in Perio­dontal Research: A Mini-Review of the Literature. The Internet Journal of Veterinary Medicine. 2007;5(1). doi:

Min-Jae Do, Kyuri Kim, Haeshin Lee, Seho Cha, Taegun Seo, Hee-Jung Park, Jeong-Soon Lee, Tae-Il Kim. Development of animal experimental periodontitis mo­dels. J Periodontal Implant Sci. 2013 Aug; 43(4): 147-52. doi:

Deshpande K, Jain A, Sharma R, et al. Diabetes and periodontitis. J. Indian. Soc. Periodontol. 2010;14(4):207-12. doi:

Dumitrescu AL, Abd-El-Aleem S, Morales-Aza B, Donaldson LF. A model of periodontitis in the rat: effect of lipopolysaccharide on bone resorption, osteoclast activity, and local peptidergic innervation. J Clin Periodontol. 2004;31:596-603.

Dan Liang Ke‑Jing Wang Zhi‑Qun Tang Run‑He Liu Fulei Zeng Miao‑Ying Cheng Qi‑Si Lian. Effects of nicotine on the metabolism and gene expression profile of Sprague‑Dawley rat primary osteoblasts. Molecular Medicine Reports. 2018 April 13;17(6):8269-81. doi:

Gulati M, Anand V, Jain N, et al. Essentials of Periodontal Medicine in Preventive Medicine. Int. J Prev. Med. 2013;4(9):988-94.

Haffajee AD, Teles RP, Patel MR, et al. Fac­tors affecting supragingival biofilm composition. I. Plaque mass. J Periodontal Res. 2009;44(4):511-9. doi:

Kato R, Ishihara Y, Kawanabe N, Sumiyo-shi K, Yoshikawa Y, Nakamura M. Gap-junction-mediated com­munication in human periodontal ligament cells. J. Dent. Res. 2013 Jul;92(7):635-40. doi:

Guvva S, Patil MB, Mehta DS Rat as labora-tory animal model in periodontology. Int J of Oral Health Sci­ence. 2017 Mar;7(2):68-75. doi:

Sculean A, Donos N, Brecx M, Karring T, Reich E. Healing of fenestration-type defects following treat­ment with guided tissue regeneration or enamel matrix proteins. an experimental study in monkeys Clin Oral Investig. 2000;4(1):50-6. doi:

Helieh S, David A. Animal Models for Perio­dontal Disease. Journal of Biomedicine and Biotechno­logy. 2011;8:23-31. doi:

Julita Maria F, Coelho Samilly S, Miranda Simo­ne S. da Cruz Soraya C. Trindade Johelle de S. Passos-Soares Eneida de M. M. Cerqueira Maria da Conceição N. Costa Ana Claúdia M. G. Figueiredo Alexandre Mar­celo Hintz Maurício L. Barreto Gregory J. Seymour Frank Scannapieco Isaac Suzart Gomes-Filho. Is there as­sociation between stress and periodontitis. Clinical Oral Investigations. 2019. 25 Oct.;1-10. doi:

Kostopoulos L, Karring T. Susceptibility of GTR-regenerated periodontal attachment to ligature-induced periodontitis J Clin Periodontol. 2004;31(5):336-40. doi:

Locker D, Slade G, Murray H. Epidemiology of periodontal diseases among older adults a review. Periodontology. 2000;16:16-33. doi:

Madden TE, Caton JG. Animal models for perio­dontal disease Methods Enzymol. 1994;235:106-9. doi:

Nakatsu S, Yoshinaga Y, Kuramoto A, Nagano F, Ichimura I, Oshino K, Yoshimura A, Yano Y, Hara Y. Oc­clusal trauma accelerates attachment loss at the onset of experimental periodontitis in rats. Journal of Periodontal Research. 2014 Jun;49(3):314-22. doi:

Manjunath BC, Praveen K, Chandrashekar BR, et al. Periodontal infections: a risk factor for various systemic diseases. Natl. Med. J India. 2011;24(4):214-9.

Tobita M, Uysal AC, Ogawa R, Hyakusoku H, Mizuno H. Periodontal tissue regeneration with adipose-derived stem cells Tissue Eng Part A. 2008;14(6):945-53. doi:

Xin-yi Zheng, Chuan-yuan Mao, Han Qiao, Xi Zhang, Li Yu, Ting-yu Wang & Er-yi Lu. Plumbagin suppresses chronic periodontitis in rats via down-regu­lation of TNF-α, IL-1β and IL-6 expression. Acta pharma­cologica sinica 2017. 29 May;38. doi:

Lallam-Laroye C, Escartin Q, Zlowodzki AS, Bar­ritault D, Caruelle JP, Baroukh B, et al. Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic. J Biomed Mater Res A. 2006;79:675-83. doi:

Selvig KA. Discussion: animal models in recon­structive therapy J Periodontol. 1994;65(12):1169-72. doi:

Struillou X, Boutigny H, Soueidan A, Layrolle P. Experimental animal models in periodontology: a review. The Open Dentistry Journal, 29 Apr 2010, 4:37-47. doi:

Hirashima S, Ohta K, Kanazawa T, Okayama S, Togo A, Uchimura N et al. Three-dimensional ultrastruc­tural analysis of cells in the periodontal ligament using focused ion beam/scanning electron microscope tomography. Sci Rep. 2016 Dec 20;6:39435. doi:

Sculean A, Donos N, Brecx M, Reich E, Karring T. Treatment of intrabony defects with guided tissue regeneration and enamel-matrix-proteins. an experimental study in monkeys. J Clin Periodontol 2000;27(7):466-72. doi:

Weinberg MA, Bral M. Laboratory animal mo­dels in periodontology. J Clin Periodontol. 1999;26:335‑40. doi:

Donos N, Sculean A, Glavind L, Reich E, Karring T. Wound healing of degree III furcation involvements following guided tissue regeneration and/or Emdogain. a histologic study J Clin Periodontol. 2003;30(12):1061-8. doi:

Yamasaki A, Nikai H, Niitani K, Ijuhin N. Ultra­structure of the junctional epithelium of germfree rat gingiva J Periodontol. 1979;50(12):641-8. doi:




How to Cite

Kopchak OV, Marchenko NS, Yanishevska YV. Modeling of chronic generalized periodontitis in laboratory animals (literature review). Med. perspekt. [Internet]. 2020Oct.5 [cited 2024Jul.23];25(3):22-9. Available from: