Microbiological profile of sputum in stable adult patients with bronchiectasis in the Dnipro region of Ukraine

Authors

DOI:

https://doi.org/10.26641/2307-0404.2020.3.214823

Keywords:

bronchiectasis, sputum, drug resistance, Pseudomonas aeruginosa

Abstract

Chronic respiratory tract infection and relapsing exacerbations worsen the quality and reduce the life expectancy of patients with bronchiectasis. This work aimed to identify the spectrum of pathogens and to determine their profile of antibiotic resistance in the sputum of patients with bronchiectasis in the Dnipro region. Sputum of 60 patients in a stable phase with confirmed bronchiectasis was a subject to microbiological examination and determination of antibiotic sensitivity according to generally accepted CLSI recommendations. According to the results of the study, it was found that 70% of patients have sputum colonization by pathogens in the stable phase of the disease, and the most common pathogens are Pseudomonas aeruginosa and Haemophilus influenzae, which is in line with the global trend. Haemophilus influenzae was sensitive to ampicillin, amoxicillin, amoxicillin/clavulanate, piperacillin/tazobactam, cefuroxime, ceftriaxone, cefotaxime, cefepime, cipro­floxacin, levofloxacin and moxifloxacin in a hundred percent of cases. However, more than half of the strains of Pseudomonas aeruginosa were resistant to one or more drugs with anti-Pseudomonas activity. In particular, the highest level of resistance was identified to such drugs as imipenem, aztreonam, ceftazidime. The problem of antibiotic resistance is alarming and once again indicates the need for the regular microbiological examination of the sputum of patients with bronchiectasis even in a stable phase for subsequent rational administration of antibacterial therapy.

Author Biographies

K. Yu. Gashynova

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Department of occupational diseases and clinical immunology
Blyzhnia str., 31, Dnipro, 49102, Ukraine

K. S. Suska

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Department of occupational diseases and clinical immunology
Blyzhnia str., 31, Dnipro, 49102, Ukraine

V. V. Dmytrychenko

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Department of occupational diseases and clinical immunology
Blyzhnia str., 31, Dnipro, 49102, Ukraine

References

Antomonov MYu. [Mathematical processing and analysis of biomedical data]. 2-e izd. Kyiv: Medinform; 2018. p. 579. Russian. Available from: https://www.olx.ua/.../antomonov-m-yu-monografya

Pertseva TO, Gashynova KYu, Dmytrychen­ko VV, et al. Bronchoectatic disease: the state of art and the clinical case. Medicni perspektivi. 2018;23(3):153-61. Ukrainian. doi: https://doi.org/10.26641/2307-0404.2018.3(part1).142360

Feshchenko YI, Gavrisyuk VK, Dziublyk IV, Dziublyk OYa. [Infectious exacerbation of chronic obstructive pulmonary disease: place and role of respiratory viral pathogens.]. Medicni perspektivi. 2019;24(4):30-35. doi: https://doi.org/10.26641/2307-0404.2019.4.189191

Vidal OS, Perea L, Cantó E, et al. Antimicrobial peptides and airway bacterial colonization in bron­chiectasis. Eur Respir J. 2018;53(Suppl62):OA4948. doi: https://doi.org/10.1183/13993003.congress-2018.OA4948

Hill AT, Sullivan AL, Chalmers JD, et al. British Thoracic Society guideline for bronchiectasis in adults. Thorax. 2019;74(Suppl. 1):1-69. doi: https://doi.org/10.1136/thoraxjnl-2018-212463

Diaz AA, Young TP, Maselli DJ, et al. Broncho­arterial ratio in never-smokers adults: implications for bronchial dilation definition. Respirology. 2017;22(1):108-13. doi: https://doi.org/10.1111/resp.12875

Clinical and Laboratory Standards Institute. CLSI M100 S27:2017. Performance standard for antimicrobial susceptibility testing. 27th edn. Wayne, PA: Clinical and Laboratory Standards Institute; 2017.

Radovanovic D, Santus P, Blasi F, et al. A com­prehensive approach to lung function in bronchiectasis. Respir Med 2018;145:120-9. doi: https://doi.org/10.1016/j.rmed.2018.10.031

Cornaglia G, Courcol R and Herrmann J-L. Euro­pean Manual of Clinical Microbiology; 2012.

Lonni S, Chalmers JD, Goeminne PC, et al. Etio­logy of non-cystic fibrosis bronchiectasis in adults and its correlation to disease severity. Ann Am Thorac Soc 2015;12:1764-70. doi: https://doi.org/10.1513/AnnalsATS.201507-472OC

Polverino E, Goeminne PC, McDonnell MJ, et al. European Respiratory Society guidelines for the mana­ge­ment of adult bronchiectasis. Eur Respir J. 2017;50:1700629. doi: https://doi.org/10.1183/13993003.00629-2017

Chandrasekaran R, Mac Aogain M, Chalmers JD, Elborn SJ, Chotirmall SH. Geographic variation in the aetiology, epidemiology and microbiology of bron­chiectasis. Bmc Pulmonary Medicine. 2018;18:14. doi: https://doi.org/10.1186/s12890-018-0638-0

Matuschek E, Brown DFJ and Kahlmeter G. Development of the EUCAST disk diffusion anti­microbial susceptibility testing method and its implementation in routine microbiology labo­ratories. Clin Microbiol Infect. 2014;20:O255-66. doi: https://doi.org/10.1111/1469-0691.12373

Gashynova K, Suska K, Dmytrychenko V. Micro­biome landscape and disease duration role in allergy in adult patients with bronchiectasis. Eur Respir J. 2019;54(Suppl63):PA2763. doi: https://doi.org/10.1183/13993003.congress-2019.PA2763

Polverino E, Rosales-Mayor E, Benegas M. Pneu­monic and non-pneumonic exacerbations in bron­chiectasis: clinical and microbiological differences. J Infect. 2018;77:99-106. doi: https://doi.org/10.1016/j.jinf.2018.04.006

Chalmers JD, Goeminne P, Aliberti S, et al. The bronchiectasis severity index. An international derivation and validation study. Am J Respir Crit Care Med 2014;189:576-85. doi: https://doi.org/10.1164/rccm.201309-1575OC

Published

2020-10-05

How to Cite

1.
Gashynova KY, Suska KS, Dmytrychenko VV. Microbiological profile of sputum in stable adult patients with bronchiectasis in the Dnipro region of Ukraine. Med. perspekt. [Internet]. 2020Oct.5 [cited 2023Dec.1];25(3):104-10. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/214823

Issue

Section

CLINICAL MEDICINE