Сomparative characteristics of lipid peroxide oxidation activity and antioxidant protection in rat’s liver on the background of experimental ischemic-reperfusion limb syndrome

Authors

DOI:

https://doi.org/10.26641/2307-0404.2020.4.221227

Keywords:

ischemia-reperfusion syndrome, liver, trauma, blood loss, haemostatic tourniquet, lipid peroxidation

Abstract

The organism’s reaction to pathogenic impacts such as blood loss and ischemia can appear either on systemic or on ultrastructural level. The tourniquet inflation pressure on the extremity triggers local lipid peroxidation. However, the systemic influence of tourniquet is not completely studied. This experiment was dedicated to study of the changes that occur in the liver on the background of ischemic-reperfusion syndrome of the limb. In our experiment the animals were divided into 5 groups, in which the effect of ischemia-limb reperfusion, blood loss, mechanical injury of the thigh bone and their combination on the concentration of malonic dialdehyde and glutatoin peroxidase activity was studied. Biochemical investigation of the liver has shown that each of these experimental interventions had caused activation of lipid peroxidation in the liver and proper increase of the activity of the antioxidant protection enzyme in the blood. The peculiarities of the reaction were an increase of the first index, which was the highest among all experimental groups – on the background of blood loss combined with the use of a tourniquet; at the same time the critical suppression of antioxidation was noted as well. Besides 2 periods of exhaustion of the wounded organism were established – on the 3rd and 14th day. At the same time, the content of malonic dialdehyde was higher in the group where the imposition of the tourniquet was combined with mechanical trauma, comparatively to isolated mechanical trauma of the thigh, this indicated the role of the tourniquet as a factor complicating the course of posttraumatic period due to ischemic reperfusion syndrome. Understanding the pathogenesis of traumatic disease will allow to find a new way of valuing of the effects of limb decompression, to cope with oxidative stress and more successfully treat multiple organ failure.

References

Antomonov M. [Mathematical processing and analysis of medical and biological data]. Kyiv; 2017. p. 19. Russian.

Volotovska NV, Hudyma A. [The role of hemic hypoxia in the pathogenesis of disorders of biliary and biliary function of the liver on the background of skeletal trauma in the early period]. Achievements of clinical and experimental medicine. 2011;2(15):31-38. Ukrainian.

Volotovska NV, Zarichna OI, Kuzmak IP. [Cata­lase and superoxide dismutase activity on the background of experimental ischemia-reperfusion of the limb]. Shpy­talna hirurhiia, Journal of them. Lya Kovalchuk. 2019;2:53-59. Ukrainian. doi: https://doi.org/10.11603/2414-4533.2019.2.10418

Ehalov VV, Stus VP, Moiseenko NP. [Boywaters Syndrome. Acute kidney injury]. Urology. 2020;24(1):68-93. Russian.

doi: https://doi.org/10.26641/2307-5279.24.1.2020.199505

[Law of the Ministry of Health of Ukraine No. 690]. Ukrainian. Available from: https://zakon.rada.gov.ua/laws/show/z1010-09#Text

Iskra RIa. [The activity of the antioxidant system in the rabbit under the action of chromium compounds]. Biological studies. 2012;6(1):77-86. Ukrainian. DOI: https://doi.org/10.30970/sbi.0601.201

Kolesova OE, Markin AA, Fedorova TN. [Lipid peroxidation and methods for determining lipid pero­xidation products in biological media Kolesova]. La­boratory work. 1984;9:540-6. Russian.

KruglIkova GO, Shtutman UM. [Methods for determining the activity of glutathione peroxidase and glutathione reductase]. Ukrainian Biochemical Journal. 1976;68(2):223-8. Ukrainian.

Kuzminskyi IV. [Influence of ischemic-reperfu­sion syndrome of limbs on deviations of indicator of cytolytic syndrome]. Achievements of clinical and experimental medicine. 2018;3:176-81. doi: https://doi.org/10.11603/1811-2471.2018.v0.i3.9351

Liubovych OYe, Klishch IM. [Features of chan­ges in the antioxidant system in the dynamics of mobi­lization stress on the background of hypothyroidism]. Bulletin of Scientific Research. 2018;4:175-9. Ukraininan.

Nebyilitsin YuS, Lazuko SS, Kutko EA. [Lower limb ischemia-reperfusion syndrome]. Vitebsk State Medical University Bulletin. 2018;17(6):18-31. Russian. doi: https://doi.org/10.22263/2312-4156.2018.6.18

Semenenko AI, Kondratskyi BO. [Changes in oxidative-antioxidant balance on the background of infusion therapy in cerebral ischemia-reperfusion]. Emer­gency medicine. 2015;2(65):116-9. Ukrainian.

Bordakov ВN, Alekseev SA, Chumanevich OA, Patsay DI, Bordakov PV. [Prolonged compression synd­rome]. Military medicine. 2013;1:6-32. Russian. Available from: http://rep.bsmu.by/handle/BSMU/2213

Tsymbaliuk HYu. [The state of daily renal diuresis in the conditions of ischemic-reperfusion synd­rome of the extremities, trauma of the abdominal organs complicated by hypovolemic shock, and their combi­nation in the early period of traumatic illness]. Achie­vements of clinical and experimental medicine. 2018;3:163-9. Ukrainian.

Kosa K, Yurttas Y, Yildiz C, Cayci T, Uysal B, Korkmaz A. Effect of hyperbaric oxygen and ozone pre­con­ditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopedica et Traumatologica Turcica. 2010;44(6):476-83. doi: https://doi.org/10.3944/AOTT.2010.2327

Fukuda I, Chiyoya M, Taniguchi S, Fukuda W. Acute limb ischemia: contemporary approach. General Thoracic and Cardiovascular Surgery. 2015;63(10):540-8. doi: https://doi.org/10.1007/s11748-015-0574-3

Kauvar DS, Dubick MA, Walters TJ, Kragh JJ. Systematic review of prehospital tourniquet use in civilian limb trauma. J Trauma Acute Care Surg. 2018;84(5):819-25. doi: https://doi.org/10.1097/TA.0000000000001826

Vaishya R, Agarval AK, Vijay V, Tiwari MK. Short term outcomes of long duration versus short du­ration tourniquet in primary total knee arthroplasty: a randomized controlled trial. Journal of Clinical Orthopaedics and Trauma. 2018;9(1):46-50. doi: https://doi.org/10.1016/j.jcot.2017.11.016

Volotovska NV, Godovana AYu, Appiah D. Nkansah. Morphological and metabolic changes caused by syndrome of ischemia-reperfusion, and features of its therapeutic influence. Zdobutki klinіchno і experimental medicine. 2018;4:26-41. doi: https://doi.org/10.11603/1811-2471.2018.v0.i4.9733

Published

2020-12-28

How to Cite

1.
Volotovska N. Сomparative characteristics of lipid peroxide oxidation activity and antioxidant protection in rat’s liver on the background of experimental ischemic-reperfusion limb syndrome. Med. perspekt. [Internet]. 2020Dec.28 [cited 2024Nov.14];25(4):39-47. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/221227

Issue

Section

THEORETICAL MEDICINE