Сomparative characteristics of lipid peroxide oxidation activity and antioxidant protection in rat’s liver on the background of experimental ischemic-reperfusion limb syndrome
DOI:
https://doi.org/10.26641/2307-0404.2020.4.221227Keywords:
ischemia-reperfusion syndrome, liver, trauma, blood loss, haemostatic tourniquet, lipid peroxidationAbstract
The organism’s reaction to pathogenic impacts such as blood loss and ischemia can appear either on systemic or on ultrastructural level. The tourniquet inflation pressure on the extremity triggers local lipid peroxidation. However, the systemic influence of tourniquet is not completely studied. This experiment was dedicated to study of the changes that occur in the liver on the background of ischemic-reperfusion syndrome of the limb. In our experiment the animals were divided into 5 groups, in which the effect of ischemia-limb reperfusion, blood loss, mechanical injury of the thigh bone and their combination on the concentration of malonic dialdehyde and glutatoin peroxidase activity was studied. Biochemical investigation of the liver has shown that each of these experimental interventions had caused activation of lipid peroxidation in the liver and proper increase of the activity of the antioxidant protection enzyme in the blood. The peculiarities of the reaction were an increase of the first index, which was the highest among all experimental groups – on the background of blood loss combined with the use of a tourniquet; at the same time the critical suppression of antioxidation was noted as well. Besides 2 periods of exhaustion of the wounded organism were established – on the 3rd and 14th day. At the same time, the content of malonic dialdehyde was higher in the group where the imposition of the tourniquet was combined with mechanical trauma, comparatively to isolated mechanical trauma of the thigh, this indicated the role of the tourniquet as a factor complicating the course of posttraumatic period due to ischemic reperfusion syndrome. Understanding the pathogenesis of traumatic disease will allow to find a new way of valuing of the effects of limb decompression, to cope with oxidative stress and more successfully treat multiple organ failure.References
Antomonov M. [Mathematical processing and analysis of medical and biological data]. Kyiv; 2017. p. 19. Russian.
Volotovska NV, Hudyma A. [The role of hemic hypoxia in the pathogenesis of disorders of biliary and biliary function of the liver on the background of skeletal trauma in the early period]. Achievements of clinical and experimental medicine. 2011;2(15):31-38. Ukrainian.
Volotovska NV, Zarichna OI, Kuzmak IP. [Catalase and superoxide dismutase activity on the background of experimental ischemia-reperfusion of the limb]. Shpytalna hirurhiia, Journal of them. Lya Kovalchuk. 2019;2:53-59. Ukrainian. doi: https://doi.org/10.11603/2414-4533.2019.2.10418
Ehalov VV, Stus VP, Moiseenko NP. [Boywaters Syndrome. Acute kidney injury]. Urology. 2020;24(1):68-93. Russian.
doi: https://doi.org/10.26641/2307-5279.24.1.2020.199505
[Law of the Ministry of Health of Ukraine No. 690]. Ukrainian. Available from: https://zakon.rada.gov.ua/laws/show/z1010-09#Text
Iskra RIa. [The activity of the antioxidant system in the rabbit under the action of chromium compounds]. Biological studies. 2012;6(1):77-86. Ukrainian. DOI: https://doi.org/10.30970/sbi.0601.201
Kolesova OE, Markin AA, Fedorova TN. [Lipid peroxidation and methods for determining lipid peroxidation products in biological media Kolesova]. Laboratory work. 1984;9:540-6. Russian.
KruglIkova GO, Shtutman UM. [Methods for determining the activity of glutathione peroxidase and glutathione reductase]. Ukrainian Biochemical Journal. 1976;68(2):223-8. Ukrainian.
Kuzminskyi IV. [Influence of ischemic-reperfusion syndrome of limbs on deviations of indicator of cytolytic syndrome]. Achievements of clinical and experimental medicine. 2018;3:176-81. doi: https://doi.org/10.11603/1811-2471.2018.v0.i3.9351
Liubovych OYe, Klishch IM. [Features of changes in the antioxidant system in the dynamics of mobilization stress on the background of hypothyroidism]. Bulletin of Scientific Research. 2018;4:175-9. Ukraininan.
Nebyilitsin YuS, Lazuko SS, Kutko EA. [Lower limb ischemia-reperfusion syndrome]. Vitebsk State Medical University Bulletin. 2018;17(6):18-31. Russian. doi: https://doi.org/10.22263/2312-4156.2018.6.18
Semenenko AI, Kondratskyi BO. [Changes in oxidative-antioxidant balance on the background of infusion therapy in cerebral ischemia-reperfusion]. Emergency medicine. 2015;2(65):116-9. Ukrainian.
Bordakov ВN, Alekseev SA, Chumanevich OA, Patsay DI, Bordakov PV. [Prolonged compression syndrome]. Military medicine. 2013;1:6-32. Russian. Available from: http://rep.bsmu.by/handle/BSMU/2213
Tsymbaliuk HYu. [The state of daily renal diuresis in the conditions of ischemic-reperfusion syndrome of the extremities, trauma of the abdominal organs complicated by hypovolemic shock, and their combination in the early period of traumatic illness]. Achievements of clinical and experimental medicine. 2018;3:163-9. Ukrainian.
Kosa K, Yurttas Y, Yildiz C, Cayci T, Uysal B, Korkmaz A. Effect of hyperbaric oxygen and ozone preconditioning on oxidative/nitrosative stress induced by tourniquet ischemia/reperfusion in rat skeletal muscle. Acta Orthopedica et Traumatologica Turcica. 2010;44(6):476-83. doi: https://doi.org/10.3944/AOTT.2010.2327
Fukuda I, Chiyoya M, Taniguchi S, Fukuda W. Acute limb ischemia: contemporary approach. General Thoracic and Cardiovascular Surgery. 2015;63(10):540-8. doi: https://doi.org/10.1007/s11748-015-0574-3
Kauvar DS, Dubick MA, Walters TJ, Kragh JJ. Systematic review of prehospital tourniquet use in civilian limb trauma. J Trauma Acute Care Surg. 2018;84(5):819-25. doi: https://doi.org/10.1097/TA.0000000000001826
Vaishya R, Agarval AK, Vijay V, Tiwari MK. Short term outcomes of long duration versus short duration tourniquet in primary total knee arthroplasty: a randomized controlled trial. Journal of Clinical Orthopaedics and Trauma. 2018;9(1):46-50. doi: https://doi.org/10.1016/j.jcot.2017.11.016
Volotovska NV, Godovana AYu, Appiah D. Nkansah. Morphological and metabolic changes caused by syndrome of ischemia-reperfusion, and features of its therapeutic influence. Zdobutki klinіchno і experimental medicine. 2018;4:26-41. doi: https://doi.org/10.11603/1811-2471.2018.v0.i4.9733
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